Results 31 to 40 of about 117,982 (263)

Atomic force microscopy: A nanobiotechnology for cellular research

Nano TransMed, 2022
: Nanobiotechnology such as atomic force microscopy (AFM) has a great application in various regimes of cell biology, offering an excellent avenue to study cellular nanotopography, nanomechanics, and nanointeraction. AFM nanotopography can provide a high
Guangzhao Guan, Yan He, Li Mei
doaj   +1 more source

E2A selectively regulates TGF‐β–induced apoptosis in KRAS‐mutant non‐small cell lung cancer

Molecular Oncology, EarlyView.
Ability to induce apoptosis by TGF‐β is frequently lost in advanced lung adenocarcinoma despite intact TGF‐β signaling. We identify E2A as a mutant KRAS–dependent mediator of resistance to TGF‐β–induced apoptosis. TGF‐β induces E2A via SMAD3 in mutant KRAS cells, and E2A silencing restores apoptosis and enhances radiation response in cell lines ...
Sergei Chuikov, Shiva Krishna Katkam, Zhefan Wang, Venkateshwar G. Keshamouni   +3 more
wiley   +1 more source

Magnetic-Dielectric Cantilevers for Atomic Force Microscopy

Nanomaterials
Atomic force microscopy (AFM) is a technique that relies on detecting forces at the nanonewton scale. It involves using a cantilever with a tiny tip at one end. This tip interacts with the short- and long-range forces of material surfaces.
Gala Sanchez-Seguame, Hugo Avalos-Sanchez, Jesus Eduardo Lugo, Eduardo Antonio Murillo-Bracamontes, Martha Alicia Palomino-Ovando, Orlando Hernández-Cristobal, José Juan Gervacio-Arciniega, Miller Toledo-Solano   +7 more
doaj   +1 more source

Energy dissipation in multifrequency atomic force microscopy

Beilstein Journal of Nanotechnology, 2014
The instantaneous displacement, velocity and acceleration of a cantilever tip impacting onto a graphite surface are reconstructed. The total dissipated energy and the dissipated energy per cycle of each excited flexural mode during the tip interaction is
Valentina Pukhova, Francesco Banfi, Gabriele Ferrini   +2 more
doaj   +1 more source

Interpreting the effects of DNA polymerase variants at the structural level

Molecular Oncology, EarlyView.
Using MAVISp and molecular dynamics simulations, we analyzed over 60 000 missense variants in POLE and POLD1 from ClinVar, COSMIC, cBioPortal, and saturation mutagenesis. Identified mechanistic indicators, including stability, binding, and long‐range, enable structural interpretation, providing ACMG‐like evidence for possible reclassification of VUS ...
Matteo Arnaudi, Karolina Krzesińska, Ludovica Beltrame, Pablo Sánchez‐Izquierdo Besora, Matteo Tiberti, Mef Nilbert, Anna Rohlin, Elena Papaleo   +7 more
wiley   +1 more source

Repulsive bimodal atomic force microscopy on polymers

Beilstein Journal of Nanotechnology, 2012
Bimodal atomic force microscopy can provide high-resolution images of polymers. In the bimodal operation mode, two eigenmodes of the cantilever are driven simultaneously.
Alexander M. Gigler, Christian Dietz, Maximilian Baumann, Nicolás F. Martinez, Ricardo García, Robert W. Stark   +5 more
doaj   +1 more source

MITF maintains genome stability in nonmelanocyte lineages

Molecular Oncology, EarlyView.
MITF is essential for melanocyte survival and acts as an oncogene in 10%–20% of melanomas. We show that MITF depletion causes genome instability in nonmelanocytic cells, leading to LATS2‐mediated P53 activation, cell cycle arrest, and apoptosis. This study highlights the role of MITF as a genome maintenance factor beyond the melanocyte lineage. Created
Drifa H. Gudmundsdottir, Adrián López García de Lomana, Thejus B. Venkatesh, Kritika Kirty, Snaevar Sigurdsson, Linda Vidarsdottir, Ramile Dilshat, Erla Sveinbjornsdottir, Snaedis Ragnarsdottir, Daniel H Magnusson, Maria R. Bustos, Eirikur Steingrimsson, Thorkell Gudjonsson, Stefan Sigurdsson   +13 more
wiley   +1 more source

Progress in the Correlative Atomic Force Microscopy and Optical Microscopy

Sensors, 2017
Atomic force microscopy (AFM) has evolved from the originally morphological imaging technique to a powerful and multifunctional technique for manipulating and detecting the interactions between molecules at nanometer resolution.
Lulu Zhou, Mingjun Cai, Ti Tong, Hongda Wang   +3 more
doaj   +1 more source

Practical applications of atomic force microscopy in biomedicine

STEMedicine, 2020
The last thirty years of progress of atomic force microscopy (AFM) applied to living matter is reviewed with a focus on potential uses in drug discovery or screening of patient samples.
Nicola Galvanetto
doaj   +1 more source

Patient therapy outcome modeling in cancer organoids is improved by cancer‐associated fibroblasts and organoid assembly convolution

Molecular Oncology, EarlyView.
Patient‐derived organoids (PDOs) from pancreatic, colorectal, and gastric cancers were used to evaluate standard and experimental therapies. Incorporating cancer‐associated fibroblasts (CAFs) into organoid cultures improved patient therapy outcome prediction.
Marcin Grochowski, Liudmyla Dolinchuk, Michał Jerzak, Albert Gandurski, Tomasz Grochowski, Weronika Wojtyś, Maciej Zadrożny, Wojciech Kaźmierczak, Małgorzata Lenarcik, Marta Matejak‐Górska, Radosław Samsel, Tomasz Olesiński, Dawid Walerych   +12 more
wiley   +1 more source