Results 41 to 50 of about 493,727 (307)

Requirement of Zn to demonstrate HCO3-stimulated ATPase activity of rat small intestinal brush border. [PDF]

, 1979
The existence of a membrane-bound HCO3-stimulated ATPase in intestinal mucosa is controversial. A crude brush border fraction of rat small intestinal homogenates contained HCO3-ATPase activity which was inhibited by preincubation with 3 mM EDTA. Alkaline
Chou, LY, Humphreys, MH, Kaysen, GA
core  

The dynamic action of SecA during the initiation of protein translocation [PDF]

, 2013
Biotechnology and Biological Sciences Research Council (BBSRC) [a doctoral training grant Ph.D. studentship to S.W. and project grant number BB/I008675/1] and the Wellcome Trust [project grant number 084452]
Alice Robson, Bauer, Blobel, Breyton, Brundage, Cannon, Deville, Ding, Erlandson, Gelis, Gold, Gold, Gouridis, Hizlan, Hunt, Ian Collinson, Kimura, Or, Osborne, Osborne, Park, Plath, Robson, Robson, Sarah Whitehouse, van den Berg, Vicki A. M. Gold, Whitehouse, Woodbury, Zimmer, Zimmer   +30 more
core   +2 more sources

AAA+ protein unfoldases—the Moirai of the proteome

FEBS Letters, EarlyView.
AAA+ unfoldases are essential molecular motors that power protein degradation and disaggregation. This review integrates recent cryo‐electron microscopy (cryo‐EM) structures and single‐molecule biophysical data to reconcile competing models of substrate translocation.
Stavros Azinas, Marta Carroni
wiley   +1 more source

Plasma membrane calcium ATPase activity is regulated by actin oligomers through direct interaction [PDF]

, 2013
As recently described by our group, plasma membrane calcium ATPase (PMCA) activity can be regulated by the actin cytoskeleton. In this study, we characterize the interaction of purified G-actin with isolated PMCA and examine the effect of G-actin during ...
Dalghi, Marianela Gisela, Fernández, Marisa Mariel, Ferreira Gomes, Mariela Soledad, Malchiodi, Emilio Luis, Mangialavori, Irene Cecilia, Rossi, Juan Pablo Francisco, Strehler, Emanuel E.   +6 more
core   +1 more source

pH‐mediated activation of the lysosomal arginine sensor SLC38A9

FEBS Letters, EarlyView.
Cells monitor nutrient levels via the lysosomal transporter SLC38A9 to activate the mechanistic target of rapamycin complex 1 (mTORC1). This study reveals that SLC38A9 function is regulated by pH. We identified histidine 544 as a critical pH sensor that undergoes conformational changes to control amino acid efflux from lysosomes; therefore, it ...
Xuelang Mu, Ampon Sae Her, Tamir Gonen
wiley   +1 more source

Structure-function relationship of KaiC around dawn

Biophysics and Physicobiology
KaiC is a multifunctional enzyme functioning as the core of the circadian clock system in cyanobacteria: its N-terminal domain has adenosine triphosphatase (ATPase) activity, and its C-terminal domain has autokinase and autophosphatase activities ...
Yoshihiko Furuike, Eiki Yamashita, Shuji Akiyama   +2 more
doaj   +1 more source

Regulation of gastric acid secretion in situ by an endogenous activator protein: Studies with activator-specific antibody [PDF]

, 2013
Monospecific polyclonal antibody was raised against a homogenous preparation of endogenous activator protein (HAF) for the gastric H+,K+-ATPase system.
Tushar K. Ray, Jyotirmoy Nandi, Larry F. Lemanski and Swapan K. Ghosh
core   +1 more source

Dammarenediol II enhances etoposide‐induced apoptosis by targeting O‐GlcNAc transferase and Akt/GSK3β/mTOR signaling in liver cancer

Molecular Oncology, EarlyView.
Etoposide induces DNA damage, activating p53‐dependent apoptosis via caspase‐3/7, which cleaves PARP1. Dammarenediol II enhances this apoptotic pathway by suppressing O‐GlcNAc transferase activity, further decreasing O‐GlcNAcylation. The reduction in O‐GlcNAc levels boosts p53‐driven apoptosis and influences the Akt/GSK3β/mTOR signaling pathway ...
Jaehoon Lee, Byung‐Cheol Han, Gi‐Bang Koo, Jihye Park, Mijin Kwon, Young Bin Park, Jae‐Mun Choi, Seung‐Ho Lee, Sangho Roh   +8 more
wiley   +1 more source

ATPase cycle and DNA unwinding kinetics of RecG helicase [PDF]

, 2012
The superfamily 2 bacterial helicase, RecG, is a monomeric enzyme with a role in DNA repair by reversing stalled replication forks. The helicase must act specifically and rapidly to prevent replication fork collapse. We have shown that RecG binds tightly
Powell, Ben, Toseland, Christopher P., Webb, Martin R   +2 more
core   +4 more sources

Subtype‐specific enhancer RNAs define transcriptional regulators and prognosis in breast cancers

Molecular Oncology, EarlyView.
This study employed machine learning methodologies to perform the subtype‐specific classification of RNA‐seq data sets, which are mapped on enhancers from TCGA‐derived breast cancer patients. Their integration with gene expression (referred to as ProxCReAM eRNAs) and chromatin accessibility profiles has the potential to identify lineage‐specific and ...
Aamena Y. Patel, Peyman Zarrineh, Nathnael T. Tuffa, Jigar H. Sheth, Sumitra Mohan, Mudassar Iqbal, Sankari Nagarajan   +6 more
wiley   +1 more source