Results 71 to 80 of about 275,929 (264)

Versatile Detection of Cellular Protein via Fluorescence Anisotropy

Advanced Science, EarlyView.
Cell Lysate Fluorescence Anisotropy (CFAST) utilizes long lifetime dye‐labeled nanobodies for rapid protein quantification in minimally purified, complex cell lysate. When coupled with cellular thermal shift, CFAST allows high‐throughput detection of endogenous protein‐small molecule engagement, facilitating the discovery of novel binders for ...
Qing Tang, Yuan‐Ping Wei, Ricky Ruiqi Ma, Na Wei, Tammy Zihan Zhou, Hilary Kung‐Yu Ho, Zafar Iqbal Bhat, Iqra Ishrat, Xiaoyu Li, Judy Wai Ping Yam, Yung Hou Wong, Qiankun Wang, Justin L. Tan   +12 more
wiley   +1 more source

Control of calcium homeostasis in Schistosoma mansoni

Memorias do Instituto Oswaldo Cruz, 2001
Calcium signalling is fundamental for muscular contractility of Schistosoma mansoni. We have previously described the presence of transport ATPases (Na+,K+-ATPase and (Ca2+-Mg2+)-ATPase) and calcium channels (ryanodine receptors - RyR) involved in ...
F Noël, VMN Cunha, CLM Silva, DL Mendonça-Silva   +3 more
doaj   +1 more source

Targeting the HSPA8‐CMA‐ATP6V1A Axis Triggers Lysosomal Hyperacidification and Catastrophic Vacuolation in Prostate Cancer

Advanced Science, EarlyView.
Our experimental evidence supports a model in which ALO targets the HSPA8‐CMA‐ATP6V1A axis to induce lysosomal hyperacidification and initiate osmotic and lipidomic stress. These changes are associated with LMP and loss of lysosomal integrity in prostate cancer cells.
Bingzheng An, Ze Gao, Shuo Chen, Liwei Meng, Chen Zhang, Kefan Song, Haochen Cui, Lei Yan, Zhiqing Fang   +8 more
wiley   +1 more source

V-ATPase Dysfunction in the Brain: Genetic Insights and Therapeutic Opportunities

Cells
Vacuolar-type ATPase (v-ATPase) is a multimeric protein complex that regulates H+ transport across membranes and intra-cellular organelle acidification. Catabolic processes, such as endocytic degradation and autophagy, strictly rely on v-ATPase-dependent
Antonio Falace, Greta Volpedo, Marcello Scala, Federico Zara, Pasquale Striano, Anna Fassio   +5 more
doaj   +1 more source

CHCHD10 Mitigates Alzheimer's Disease‐Related Phenotypes in Association With Epigenetic Remodeling in Directly Reprogrammed Neurons

Advanced Science, EarlyView.
CHCHD10 loss in Alzheimer's disease is associated with mitochondrial dysfunction, epigenomic disruption, and tau pathology. Restoration of CHCHD10 shifts DNA methylation toward a non‐disease state and reduces tau and amyloid pathology, with KATNAL2 acting as a downstream effector.
Teresa M. Thomas, Ching‐Yao Yang, Kexin Zhang, Liam Wetzel, Dina Bugybayeva, Julia Ferguson, Md Mahmudul Hasan, William Samsa, Nandita Patil, Anshul Dash, Tarini Gowda, Xiongwei Zhu, Feixiong Cheng, Tian Liu   +13 more
wiley   +1 more source

The E3 Ubiquitin Ligase RLIM Safeguards Oligodendrocyte Development and Myelination by Targeting SLC7A11 for Polyubiquitination to Regulate Ferroptotic Resistance

Advanced Science, EarlyView.
RLIM‐mediated SLC7A11 polyubiquitination is required for SPTBN2‐mediated trafficking and membrane localization of SLC7A11, which enables oligodendrocyte precursor cells to be resistant to ferroptosis and safeguards oligodendrocyte lineage progression and thus myelination.
Yuwei Li, Haijun Zhang, Jieya Zhou, Shiyu Yang, Jingmei Cha, Gaofeng Yan, Shuhua Zhao, Bingyu Mao, Pengcheng Ma   +8 more
wiley   +1 more source

MAM‐Localized MANF Counteracts Microinflammatory Stress to Attenuate Mitochondrial Dysfunction and Cataractogenesis in High Myopia

Advanced Science, EarlyView.
Chronic microinflammation in high myopia suppresses MANF in lens epithelial cells. MANF normally resides within MAMs and promotes ubiquitin‐mediated degradation of the ER Ca2+ pump SERCA2. MANF loss causes pathological SERCA2 accumulation, MAM hyperassembly, and disrupted ER‐to‐mitochondria Ca2+ transfer, leading to mitochondrial failure, oxidative ...
Xin Liu, Hao Li, Ching Kang, Dongling Guo, Shuyu Liu, Jiaqi Meng, Zhiqian Huang, Yu Du, Xiangjia Zhu   +8 more
wiley   +1 more source

Investigation of the Proteasome 26S Subunit, ATPase Family Genes as Potential Prognostic Biomarkers and Therapeutic Targets for Hepatocellular Carcinoma

Cancer Management and Research
Lei Feng,1,* Ning Zhang,1,* Lan Luo,1,* Jie Liu,1,* Yong Yao,1,* Ming-Sheng Gao,1,* Jin Pan,1,* Cai He2 1The Division of Gastroenterology and Hepatology, Suining Central Hospital, Suining City, Sichuan Province, People’s ...
Feng L, Zhang N, Luo L, Liu J, Yao Y, Gao MS, Pan J, He C   +7 more
doaj  

Effect of Electroacupuncture of "Neiguan"(PC 6) on Na~+-K~+-ATPase Activity and Its Gene Expression in Cardiocyte Membrane in Rats with Myocardial Ischemia-reperfusion Injury

针刺研究, 2007
Objective: To compare the effects of electroacupuncture(EA) of "Neiguan" and other acupoints on Na+-K+-ATPase activity and its gene expression in ischemic myocrardial cell membrane in rats.Methods: A total of 50 Wistar rats were randomly divided into ...
YAN Jie, YANG Xiao-fang1, YI Shou-xiang, CHANG Xiao-rong, LIN Ya-ping, WANG Chao, DIAO Li-hong2   +6 more
doaj  

The impact of 3-sulfo-taurolithocholic acid on ATPase activity in patients’ colorectal cancer and normal colon tissues, and its hepatic effects in rodents

Frontiers in Veterinary Science
Colorectal cancer is influenced by genetic mutations, lifestyle factors, and diet, particularly high fat intake, which raises bile acid levels in the intestinal lumen.
Solomiia Bychkova, Mykola Bychkov, Dani Dordević, Simon K.-M. R. Rittmann, Monika Vítězová, Ivan Kushkevych   +5 more
doaj   +1 more source