Results 81 to 90 of about 57,206 (302)
Measuring Autophagic Cargo Flux with Keima-Based Probes
, 2022 Autophagy and autophagy-associated genes are implicated in a growing list of cellular, physiological, and pathophysiological processes and conditions. Therefore, it is ever more important to be able to reliably monitor and quantify autophagic activity. Whereas autophagic markers, such as LC3 can provide general indications about autophagy, specific and
Engedal, Nikolai +8 more
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Snazarus and its human ortholog SNX25 modulate autophagic flux
Journal of Cell Science, 2021 ABSTRACT Macroautophagy, the degradation and recycling of cytosolic components in the lysosome, is an important cellular mechanism. It is a membrane-mediated process that is linked to vesicular trafficking events. The sorting nexin (SNX) protein family controls the sorting of a large array of cargoes, and various SNXs impact autophagy ...
Annie Lauzier +6 more
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Cell type-specific autophagy in human leukocytes: signatures of aging, sex, and nutrient restriction
Autophagy ReportsMacroautophagy (referred to here as autophagy) is thought to play a critical role in aging and age-related disease, making it a priority for development of targeted human therapies. We developed a flow cytometry-based method to measure autophagic flux in
Linh VP Dang, Timothy J Sargeant
doaj +1 more source
Frontiers in Cell and Developmental Biology, 2021 Background: Cardiac autophagic flux is impaired during myocardial ischemia/reperfusion (MI/R). Impaired autophagic flux may exacerbate MI/R injury. Charged multivesicular body protein 2B (CHMP2B) is a subunit of the endosomal sorting complex required for
Tian Li +9 more
doaj +1 more source
Fucosylation of LAMP-1 and LAMP-2 by FUT1 correlates with lysosomal positioning and autophagic flux of breast cancer cells. [PDF]
, 2016 Alpha1,2-fucosyltransferases, FUT1 and FUT2, which transfer fucoses onto the terminal galactose of N-acetyl-lactosamine via α1,2-linkage have been shown to be highly expressed in various types of cancers.
Cho, Huan-Chieh +5 more
core
The mitochondrial protease HtrA2 restricts the NLRP3 and AIM2 inflammasomes. [PDF]
, 2018 Activation of the inflammasome pathway is crucial for effective intracellular host defense. The mitochondrial network plays an important role in inflammasome regulation but the mechanisms linking mitochondrial homeostasis to attenuation of inflammasome ...
Alnemri, Emad S. +9 more
core +4 more sources
Advanced Healthcare Materials, EarlyView.Lysosome‐targeted acidic nanoparticles based on a biodegradable poly(ethylene tetrafluorosuccinate‐co‐succinate) copolymer are engineered to restore impaired lysosomal acidification through pH‐responsive intracellular degradation. Localized acid release enhances autophagic proteolysis, reduces α‐synuclein accumulation, and preserves dopaminergic neuron
Chih Hung Lo +6 more
wiley +1 more source
Quantitative Analysis of Autophagic Flux by Ratiometric pH-Imaging of Autophagic Intermediates [PDF]
Biophysical Journal, 2016 Although numerous techniques have been developed to monitor autophagy to probe its cellular functions, these methods can’t evaluate in sufficient detail the autophagy process, and suffer limitations from complex experimental setups and/or systematic errors.
Maulucci, Giuseppe +8 more
openaire +1 more source
International Journal of Nanomedicine, 2017 Jae-Kyo Jeong,1 You-Jin Lee,1 Seung Yol Jeong,2,3 Sooyeon Jeong,2 Geon-Woong Lee,2 Sang-Youel Park1 1Biosafety Research Institute, College of Veterinary Medicine, Chonbuk National University, Iksan, 2Nano Hybrid Technology Research Center, Korea ...
Jeong JK +5 more
doaj
Scientific Reports, 2021 ALK inhibitors effectively target EML4-ALK positive non-small cell lung cancer, but their effects are hampered by treatment resistance. In the present study, we asked whether ALK inhibition affects autophagy, and whether this may influence treatment ...
Anna M. Schläfli +6 more
doaj +1 more source