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Freeze-fracture-autoradiography

Histochemistry, 1976
A new method for the electron microscope autoradiography of soluble substances in frozen tissue is described. The basic features of the method are freeze fracturing, the application of a suitable monolayer followed by exposure at low temperature and finally the separation of tissue and the replica-monolayer-sandwich after photographic processing.
Roland Taugner, Eckhard Rix, A. Schiller
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Specification of cerebral cortical areas.

Science, 1988
How the immense population of neurons that constitute the human cerebral neocortex is generated from progenitors lining the cerebral ventricle and then distributed to appropriate layers of distinctive cytoarchitectonic areas can be explained by the ...
P. Rakic
semanticscholar   +1 more source

Methods for brain autoradiography☆

Physiology & Behavior, 1973
Abstract Techniques describing the preparation of neural tissue for autoradiographic analyses are reviewed under the categories of emulsion coating, brain removal, microtome-cryostat procedure, exposure and development. Unfixed brain tissue sliced at 2–6 μ is mounted on emulsion coated slides under safelight conditions.
Cornelius C. Meyer, Olita N. Meyer
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Multiplexing Autoradiography

2023
Autoradiography, the direct imaging of radioactive distribution in tissue sections, is a powerful technique that has several key advantages for the validation of PET radiotracers. Using autoradiography, we can localize radiotracer uptake to neighbours of cells, and when multiplexed with additional radiotracers, fluorescent probes, or in situ tissue ...
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Double label autoradiography

Analytical Biochemistry, 1974
Abstract A method is described for double label autoradiography of thin-layer chromatograms. The method relies upon the ability to increase the sensitivity of 3H detection following treatment of the chromatography plate with a scintillation fluor without simultaneously increasing the sensitivity to 14C.
Eric Gruenstein   +3 more
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Autoradiography with Polaroid Film

Beiträge zur Pathologie, 1973
Summary Informative autoradiograms can be obtained by applying Polaroid photographic films to tissue slices from patients given radionuclides for diagnosis or treatment. However, conventional X-ray films are more sensitive for this purpose, and are preferred if facilities are available.
Brenda Pritchard, Bill Nelson
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Measurement of the distribution volume of gadopentetate dimeglumine at echo-planar MR imaging to quantify myocardial infarction: comparison with 99mTc-DTPA autoradiography in rats.

Radiology, 1999
PURPOSE To measure the fractional distribution volume of gadopentetate dimeglumine in normal and reperfused infarcted myocardium at magnetic resonance (MR) imaging by using the fractional distribution volume of technetium 99m ...
H. Arheden   +7 more
semanticscholar   +1 more source

AUTORADIOGRAPHY

1999
Publisher Summary Electron microscope autoradiography requires the consideration of several technical factors, including not only the choice of isotope but also the choice of tissue preparation procedure, choice of photographic emulsion and its application onto the ultrathin sections, type of development, and evaluation of qualitative or quantitative ...
Arvid B. Maunsbach, Björn A. Afzelius
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Autoradiography with Tritium

1963
In autoradiography, a photographic emulsion is placed in contact with a specimen containing radioactivity and stored in the dark. The radiation, passing through the emulsion, sensitizes silver bromide granules so that they can be reduced to metallic silver by a photographic developer.
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Database management in autoradiography

Computerized Medical Imaging and Graphics, 1989
Experiments in autoradiography involve the use of radiotracers to achieve a "functional mapping" between structures of the central nervous system and observed behavior in animals. A typical experiment produces 100-300 sections per animal. Computer systems such as DUMAS (Drexel's Unix based iMage Analysis System) are used to analyze these sections. Each
Kumar Shivaramakrishnan, Oleh J. Tretiak
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