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Recombinant avidin and avidin–fusion proteins
Biomolecular Engineering, 1999Both chicken egg-white avidin and its bacterial relative streptavidin are well known for their extraordinary high affinity with biotin (Kd approximately 10(-15) M). They are widely used as tools in a number of affinity-based separations, in diagnostic assays and in a variety of other applications. These methods have collectively become known as (strept)
K J, Airenne +2 more
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Avidin-HRP conjugates in biotin-avidin immunoenzyme cytochemistry
Histochemistry, 1986Avidin-HRP conjugates were prepared, analysed and tested for avidin-biotin immunocytochemistry. Suitable biotinylation of enzymes, antigens and antibody was obtained by reacting biotin at equimolar ratio to epsilon aminogroups in proteins. The avidin-biotin interaction was used for immunocytochemical detection of phenomena in the field of immunology, i.
D M, Boorsma +2 more
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Nature, 1958
AVIDIN has been shown to bind the vitamin biotin, but the mechanism of binding is still unknown. Modification of the avidin molecule by acetylation, esterifieation or by blocking the amide, guanidyl, imidazole and phenolic groups did not result in a loss of its ability to bind biotin1.
D V, SIVA SANKAR +3 more
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AVIDIN has been shown to bind the vitamin biotin, but the mechanism of binding is still unknown. Modification of the avidin molecule by acetylation, esterifieation or by blocking the amide, guanidyl, imidazole and phenolic groups did not result in a loss of its ability to bind biotin1.
D V, SIVA SANKAR +3 more
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Hormonal Properties of Avidin-Biotinylinsulin and Avidin-Biotinylcorticotropin Complexes
Hoppe-Seyler´s Zeitschrift für physiologische Chemie, 1981In connection with the development of affinity columns (based on the avidin-biotin interaction) for retrieval of peptide and protein hormone receptors, the hormonal properties of a number of avidin-biotinylinsulin and avidin-bioinylcorticotropin complexes were examined.
F M, Finn +3 more
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Avidin–Biotin Technology: Preparation of Avidin Conjugates
2003The second step required in the application of avidin-biotin technology is to prepare an appropriate avidin-associated probe or probes for the desired application (for general reviews, see refs. 1 and 2). For example, a fluorescent form of avidin can be used for fluorescence microscopy, fluorescence-activated cell sorting, and in some cases, for ...
E A, Bayer, M, Wilchek
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Avidin. II. Composition and mode of action of avidin A
Archives of Biochemistry and Biophysics, 1952Abstract Avidin A is a glycoprotein containing mannose and hexosamine. Data concerning its amino acid composition and its amino, acidic, and basic groups are given. Avidin XA, while similar in most analytical respects, contains galactose also. A variety of chemical modification reactions did not appreciably affect the biotin-binding activity of ...
H, FRAENKEL-CONRAT +2 more
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Dual‐affinity avidin molecules
Proteins: Structure, Function, and Bioinformatics, 2005AbstractA recently reported dual‐chain avidin was modified further to contain two distinct, independent types of ligand‐binding sites within a single polypeptide chain. Chicken avidin is normally a tetrameric glycoprotein that binds water‐soluble d‐biotin with extreme affinity (Kd ≈ 10−15 M). Avidin is utilized in various applications and techniques in
Vesa P, Hytönen +10 more
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Preparation of Avidin Conjugates
1996The high-affinity avidin-biotin system has found applications in different fields of biotechnology, including immunoassays, histochemistry, affinity chromatography, and drug delivery, to name a few. A brief description of avidin and avidin-like molecules, streptavidin, deglycosylated avidin, and NeutraLite avidin is presented in the Chapter 2.
R P, Haugland, M K, Bhalgat
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Avidin-Biotin Immobilisation Systems
1993Abstract Over the past two decades, the avid in–biotin system has evolved to be an extremely useful tool in the fields of biology, biochemistry, and biotechnology. The extraordinary affinity (Kd ∼ 10−15 M) of avid in (or its bacterial relative, streptavidin) for the vitamin biotin forms the basis for using this system.
Meir Wilchek, Edward A Bayer
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