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Azure B-eosin staining of blood cells: the effects of variation in stain formulation and staining technique on stain performance.

Analytical and quantitative cytology, 1980
A quantitative study of azure B-eosin-stained blood cells is reported. The effects of variation in stain formulation and staining technique on the binding of azure B and eosin by acidophilic, basophilic and neutrophilic substrates were measured by scanning microdensitometry.
S A, Bentley   +3 more
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Staining of DNA-phosphate groups with a mixture of azure A and acridine yellow.

Microscopica acta, 1980
This paper deals with staining of DNA-phosphate groups with a mixture of an equal parts of aqueous solution of azure A and acridine yellow in a 1:1 proportion and also embodies a study of the absorption properties of the stained nuclei. It also embodies results of sequential staining of nuclei stained first with azure A followed by staining with ...
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Endoscopic observation of the gastric mucus in vivo stained with azure A.

The American journal of gastroenterology, 1976
Gastric mucus was stained with Azure A, a cationic dye, which had the highest affinity with macromolecular constituents of the mucus, under such conditions as 0.2% Azure A-0.5% NaHCO3 solution (pH 8.1) in dye concentration, staining for ten minutes, 37 degrees C in reaction temperature and the salt concentration and ionic strength below 6.0 x 10(-2 ...
T, Kamada   +4 more
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Microspectrophotometric Studies of Romanowsky Stained Blood Cells. III. The Action of Methylene Blue and Azure B

Stain Technology, 1984
The performances of two standardized Romanowsky stains (azure B/eosin and azure B/methylene blue/eosin) have been compared with each other and with a methylene blue/eosin stain. Visible-light absorbance spectra of various hematological substrates have been measured.
P N, Marshall, W, Galbraith
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A Simple Methylene Blue-Azure Ii-Basic Fuchsin Stain for Epoxy-Embedded Tissue Sections

Stain Technology, 1974
One micron-thick sections of tissues fixed in glutaraldehyde, or in glutaraldehyde followed by osmium tetroxide, and embedded in a variety of plastic resins were stained in a methylene blue-azure II solution at 65 C, then counterstained in 0.05% basic fuchsin in 2.5% ethanol at room temperature (24 C). Considerable variation was found in methylene blue-
C D, Humphrey, F E, Pittman
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Sulfation-Hematoxylin-Azure B as an Alternative to Periodic Acid-Schiff Staining

Laboratory Medicine, 1989
Gentle sulfation of formalin-fixed tissue sections followed by staining in a hematoxylin-azure B sequence results in bright blue staining of tissue elements that stain with the periodic acid-Schiff reaction—especially basement membranes. The stain is relatively rapid, simple, and permanent, is performed with inexpensive, readily available laboratory ...
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[Can azur-B eosin replace the May-Grünwald-Giemsa stain?].

Blut, 1976
A new method is described for staining blood and bone marrow smears. It is characterized by the presence of only two dyes, purified azure B and eosin in methanol, as stock solutions. Staining results are equivalent to those obtained by using the traditional dye mixtures according to May and Grunwald, Giemsa, Leishman or Wright.
D, Wittekind, V, Kretschmer, W, Löhr
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Azure B staining of DNA-aldehyde molecules of acid-hydrolysed tissue sections.

Microscopica acta, 1981
This communication presents a method for the preparation of azure B-SO2 with trichloracetic acid (TCA) and potassium metabisulphite for in situ demonstration of DNA-aldehyde molecules following acid hydrolysis of tissue sections. The shelf-life of such a dye-reagent is slightly more than that of the control, prepared with N HCl and potassium ...
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Comparative colorimetry in cytophotometric measurements of azure B-eosin Y-stained and Giemsa-stained blood cell smears.

Analytical and quantitative cytology, 1982
The advantages of the Commission Internationale de l'Eclairage (CIE) color measurements and the use of the standardizable purified azure B-eosin Y stain, as compared to the commonly used commercial Giemsa stain, were studied. Color measurement and analysis according to the CIE standards allows the comparison of color measurements from different slides ...
A, Rüter   +4 more
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