Results 41 to 50 of about 32,904 (275)

Metabolism‐Based Biomarkers for Rapid Phenotypic Antibiotic Susceptibility Testing

open access: yesAdvanced Science, EarlyView.
Metabolic differences emerging after antibiotic exposure provide rapid phenotypic signals of susceptibility and resistance. This review summarizes metabolic biomarkers for rapid phenotypic AST, associated with nutrient uptake, respiratory activity, metabolic reprogramming, and enzymatic function. ABSTRACT The accelerating global crisis of antimicrobial
Sha Yu   +7 more
wiley   +1 more source

Strain specific Agrobacterium-mediated genetic transformation of Bacopa monnieri

open access: yesJournal of Genetic Engineering and Biotechnology, 2014
Agrobacterium-mediated genetic transformation is the most preferred strategy utilized for plant genetic transformation. The present study was carried out to analyze the influence of three different strains of Agrobacterium tumefaciens on genetic ...
Sheetal Yadav   +4 more
doaj   +1 more source

Transient b-glucuronidase activity after infiltration of Arabidopsis thaliana by Agrobacterium tumefaciens

open access: yesBiologia plantarum, 1997
Transient expression of the β-glucuronidase (GUS) gene introduced into Arabidopsis thaliana intact plants by T-DNA after vacuum infiltration of Agrobacterium tumefaciens was followed. The first incidence of GUS activity was found 2 - 3 d after treatment and a peak of activity one week after treatment in both A. thaliana races, Columbia and C24.
S. Rakouský   +3 more
openaire   +1 more source

Use of the KlADH4 promoter for ethanol-dependent production of recombinant human serum albumine in Kluyveromyces lactis [PDF]

open access: yes, 1999
KlADH4 is a gene of Kluyveromyces lactis encoding a mitochondrial alcohol dehydrogenase activity which is specifically induced by ethanol. The promoter of this gene was used for the expression of heterologous proteins in K.
CRISA A.   +6 more
core  

Metabolic fate of drugs of abuse and new psychoactive substances: A pilot study on a novel workflow using a zebrafish embryo model combined with human microdosing

open access: yesBritish Journal of Clinical Pharmacology, EarlyView.
Aim The aim of this study was to develop a novel workflow to identify human urine biomarkers for drugs of abuse and new psychoactive substances. Metabolites of amphetamine, cocaine, LSD, MDMA, methamphetamine, THC, MDMB‐CHMICA, and MDPPP were first identified in a zebrafish embryo (ZE) metabolism study followed by comparison to most abundant human ...
Simon K. Wellenberg   +7 more
wiley   +1 more source

Selection of co-transformed Dendrobium Sonia 17 using hygromycin and green fluorescent protein

open access: yesBiologia Plantarum, 2011
Dendrobium Sonia 17 calluses were used for co-transformation study using particle bombardment. The bombarded transformed callus tissues were selected using half-strength MS medium containing 25 mg dm-3 hygromycin. Expression of green fluorescent protein (
C. S. Tee   +3 more
doaj   +1 more source

Development of a Sensitive and Reliable UHPLC-MS/MS Method for the Determination of Multiple Urinary Biomarkers of Mycotoxin Exposure

open access: yesToxins, 2020
A variety of mycotoxins from different sources frequently contaminate farm products, presenting a potential toxicological concern for animals and human. Mycotoxin exposure has been the focus of attention for governments around the world.
Zhezhe Liu   +6 more
doaj   +1 more source

Systematic mutational analysis of the cation-independent mannose 6-phosphate/insulin-like growth factor II receptor cytoplasmic domain - An acidic cluster containing a key aspartate is important for function in lysosomal enzyme sorting [PDF]

open access: yes, 1997
We have used systematic mutational analysis to identify signals in the 166-residue murine cation-independent mannose 6-phosphate/insulin-like growth factor II receptor cytoplasmic domain required for efficient sorting of lysosomal enzymes.
Chen, Hong Jung   +2 more
core  

Screening of Arabidopsis mutants for functional genomic studies [PDF]

open access: yes, 2008
Eight photosynthetic Arabidopsis mutants were screened for co-segregation of a photosynthetic phenotype with the T-DNA insertion. These mutants were selected from 80 photosynthetic mutants with genetic background of Columbia-0.
Ahmad, Rashid   +6 more
core   +1 more source

Endogenous melatonin partial metabolic clearance as a potential endogenous marker of CYP1A2 activity

open access: yesBritish Journal of Clinical Pharmacology, EarlyView.
Aims Cytochrome P450 1A2 (CYP1A2) exhibits substantial interindividual variability, necessitating appropriate phenotyping strategies for personalized pharmacotherapy. This study evaluated endogenous melatonin partial metabolic clearance (CLm(MEL)), calculated from urinary 6‐hydroxymelatonin (6‐O‐MEL) excretion and plasma melatonin (MEL) exposure, as a ...
Akitomo Yokokawa   +5 more
wiley   +1 more source

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