Monitoring Bacterial Conjugation by Optical Microscopy [PDF]
Frontiers in Microbiology, 2021 Bacterial conjugation is the main mechanism for horizontal gene transfer, conferring plasticity to the genome repertoire. This process is also the major instrument for the dissemination of antibiotic resistance genes. Hence, gathering primary information
Gerardo Carranza +5 more
doaj +9 more sources
The Effect of Outer Space and Other Environmental Cues on Bacterial Conjugation [PDF]
Microbiology Spectrum, 2023 Bacterial conjugation is one of the most abundant horizontal gene transfer (HGT) mechanisms, playing a fundamental role in prokaryote evolution. A better understanding of bacterial conjugation and its cross talk with the environment is needed for a more ...
Bar Piscon +9 more
doaj +3 more sources
Targeted bacterial conjugation mediated by synthetic cell-to-cell adhesions. [PDF]
Nucleic Acids Res, 2022 Genetic interventions on microbiomes, for clinical or biotechnological purposes, remain challenging. Conjugation-based delivery of genetic cargo is still unspecific and limited by low conjugation rates.
Robledo M +6 more
europepmc +4 more sources
Protein Dynamics in F-like Bacterial Conjugation [PDF]
Biomedicines, 2020 Efficient in silico development of novel antibiotics requires high-resolution, dynamic models of drug targets. As conjugation is considered the prominent contributor to the spread of antibiotic resistance genes, targeted drug design to disrupt vital ...
Nicholas Bragagnolo +6 more
doaj +3 more sources
Transgene-free genome editing in marine algae by bacterial conjugation - comparison with biolistic CRISPR/Cas9 transformation. [PDF]
Sci Rep, 2018 The CRISPR/Cas9 technology has opened the possibility for targeted genome editing in various organisms including diatom model organisms. One standard method for delivery of vectors to diatom cells is by biolistic particle bombardment.
Sharma AK +4 more
europepmc +4 more sources
Bacterial Conjugation Protocol for Ruminant Mycoplasmas [PDF]
Bio-Protocol, 2021 In Mycoplasma agalactiae, two simultaneous processes of DNA transfer have been described that require direct cell-to-cell contact and are similar to conjugation.
Eveline Sagné +2 more
doaj +6 more sources
Conjugation inhibitors compete with palmitic acid for binding to the conjugative traffic ATPase TrwD, providing a mechanism to inhibit bacterial conjugation. [PDF]
J Biol Chem, 2018 Bacterial conjugation is a key mechanism by which bacteria acquire antibiotic resistance. Therefore, conjugation inhibitors (COINs) are promising compounds in the fight against the spread of antibiotic resistance genes among bacteria.
García-Cazorla Y +6 more
europepmc +4 more sources
Molecular Mechanisms Influencing Bacterial Conjugation in the Intestinal Microbiota [PDF]
Frontiers in Microbiology, 2021 Bacterial conjugation is a widespread and particularly efficient strategy to horizontally disseminate genes in microbial populations. With a rich and dense population of microorganisms, the intestinal microbiota is often considered a fertile environment ...
Kevin Neil +2 more
doaj +3 more sources
Tanzawaic Acids, a Chemically Novel Set of Bacterial Conjugation Inhibitors. [PDF]
PLoS ONE, 2016 Bacterial conjugation is the main mechanism for the dissemination of multiple antibiotic resistance in human pathogens. This dissemination could be controlled by molecules that interfere with the conjugation process.
María Getino +7 more
doaj +3 more sources
A High-Throughput Method for Screening for Genes Controlling Bacterial Conjugation of Antibiotic Resistance [PDF]
mSystems, 2020 The rapid horizontal transmission of antibiotic resistance genes on conjugative plasmids between bacterial host cells is a major cause of the accelerating antibiotic resistance crisis.
Hanna Alalam +14 more
doaj +3 more sources