Results 1 to 10 of about 91,670 (264)

Genetic basis of I-complex plasmid stability and conjugation

PLoS Genetics, 2023
Plasmids are major drivers of increasing antibiotic resistance, necessitating an urgent need to understand their biology. Here we describe a detailed dissection of the molecular components controlling the genetics of I-complex plasmids, a group of ...
Zheng Jie Lian, Minh-Duy Phan, Steven J. Hancock, Nguyen Thi Khanh Nhu, David L. Paterson, Mark A. Schembri   +5 more
doaj   +7 more sources

Mechanistic insights into plasmid transfer inhibition in Enterobacterales by nucleoside analogues [PDF]

npj Antimicrobials and Resistance
Antimicrobial resistance (AMR) poses a major global health threat, with carbapenem-resistant and extended-spectrum β-lactamase (ESBL)-producing Enterobacterales causing widespread infections and deaths. Much of this resistance spreads through conjugative
Ilyas Alav, Ayesha Ashraf, Parisa Pordelkhaki, Michelle M. C. Buckner   +3 more
doaj   +2 more sources

Enhancing conjugation from E. coli to Streptomyces coelicolor by incorporating traJ into mobilizable plasmids [PDF]

Applied Microbiology and Biotechnology
Streptomyces species are prolific producers of bioactive natural products, yet their genetic manipulation remains constrained by inefficient DNA delivery methods in many strains.
Paula Valdés-Chiara, Yago Concha, Sergio Alonso-Fernández, Angel Manteca, Gemma Fernández-García   +4 more
doaj   +2 more sources

A complex genetic switch involving overlapping divergent promoters and DNA looping regulates expression of conjugation genes of a gram-positive plasmid. [PDF]

PLoS Genetics, 2014
Plasmid conjugation plays a significant role in the dissemination of antibiotic resistance and pathogenicity determinants. Understanding how conjugation is regulated is important to gain insights into these features.
Gayetri Ramachandran, Praveen K Singh, Juan Roman Luque-Ortega, Luis Yuste, Carlos Alfonso, Fernando Rojo, Ling J Wu, Wilfried J J Meijer   +7 more
doaj   +1 more source

Multiple Layered Control of the Conjugation Process of the Bacillus subtilis Plasmid pLS20

Frontiers in Molecular Biosciences, 2021
Bacterial conjugation is the main horizontal gene transfer route responsible for the spread of antibiotic resistance, virulence and toxin genes.
Wilfried J. J. Meijer, D. Roeland Boer, Saúl Ares, Carlos Alfonso, Fernando Rojo, Juan R. Luque-Ortega, Ling Juan Wu   +6 more
doaj   +1 more source

GENETIC ASPECTS OF INDIRECT HYPERBILIRUBINAEMIA IN NEWBORNS

Slovenska pediatrija, 2022
Indirect hyperbilirubinaemia is a common finding in the neonatal period. There are numerous causes of neonatal indirect hyperbilirubinemia, from excessive bilirubin production, impaired hepatic bilirubin uptake or bilirubin conjugation and increased ...
Katja Jarc Georgiev, Manca Velkavrh
doaj   +1 more source

Systematic investigation of recipient cell genetic requirements reveals important surface receptors for conjugative transfer of IncI2 plasmids

Communications Biology, 2023
Bacterial conjugation is a major horizontal gene transfer mechanism. While the functions encoded by many conjugative plasmids have been intensively studied, the contribution of recipient chromosome-encoded genes remains largely unknown. Here, we analyzed
Nancy Allard, Arianne Collette, Josianne Paquette, Sébastien Rodrigue, Jean-Philippe Côté   +4 more
doaj   +1 more source

Genome-Wide Association Study Reveals Host Factors Affecting Conjugation in Escherichia coli

Microorganisms, 2022
The emergence and dissemination of antibiotic resistance threaten the treatment of common bacterial infections. Resistance genes are often encoded on conjugative elements, which can be horizontally transferred to diverse bacteria.
Laetitia Van Wonterghem, Matteo De Chiara, Gianni Liti, Jonas Warringer, Anne Farewell, Natalie Verstraeten, Jan Michiels   +6 more
doaj   +1 more source

A High-Throughput Method for Screening for Genes Controlling Bacterial Conjugation of Antibiotic Resistance

mSystems, 2020
The rapid horizontal transmission of antibiotic resistance genes on conjugative plasmids between bacterial host cells is a major cause of the accelerating antibiotic resistance crisis.
Hanna Alalam, Fabrice E. Graf, Martin Palm, Marie Abadikhah, Martin Zackrisson, Jonas Boström, Alfred Fransson, Chris Hadjineophytou, Linnéa Persson, Simon Stenberg, Matilda Mattsson, Payam Ghiaci, Per Sunnerhagen, Jonas Warringer, Anne Farewell   +14 more
doaj   +1 more source

Isolation and Analysis of Donor Chromosomal Genes Whose Deficiency Is Responsible for Accelerating Bacterial and Trans-Kingdom Conjugations by IncP1 T4SS Machinery

Frontiers in Microbiology, 2021
Conjugal transfer is a major driving force of genetic exchange in eubacteria, and the system in IncP1-type broad-host-range plasmids transfers DNA even to eukaryotes and archaea in a process known as trans-kingdom conjugation (TKC).
Fatin Iffah Rasyiqah Mohamad Zoolkefli, Kazuki Moriguchi, Kazuki Moriguchi, Yunjae Cho, Kazuya Kiyokawa, Shinji Yamamoto, Katsunori Suzuki, Katsunori Suzuki   +7 more
doaj   +1 more source