Results 11 to 20 of about 91,670 (264)
Conjugation-based genetic manipulation of Fusobacterium animalis
mBioFusobacterium is an oncobacterium associated with colorectal cancer (CRC) development. Despite that F. animalis (Fa) dominates the CRC niche in humans, studies on the pathogenesis of Fusobacterium have been limited by the paucity of genetically tractable
Duhyun Ko, Wendy S. Garrett
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Conjugation-mediated genetic exchange in Legionella pneumophila [PDF]
Journal of Bacteriology, 1985 Genetic exchange mechanisms, to our knowledge, have not been reported for Legionella pneumophila, and consequently, studies on the genetic organization of L. pneumophila have not appeared in the literature. Here, we describe gene transfer mediated by broad host range conjugative plasmids in Legionella spp. Escherichia coli strains carrying plasmids RP1
L A, Dreyfus, B H, Iglewski
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Ordering the bestiary of genetic elements transmissible by conjugation [PDF]
Mobile Genetic Elements, 2013 Phylogenetic reconstruction of three highly conserved proteins involved in bacterial conjugation (relaxase, coupling protein and a type IV secretion system ATPase) allowed the classification of transmissible elements in relaxase MOB families and mating pair formation MPF groups.
Maria Pilar Garcillán-Barcia +1 more
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Genetic and physiological analysis of conjugation in Streptococcus faecalis [PDF]
Journal of Bacteriology, 1982 In an effort to elucidate the mechanisms of conjugal plasmid transfer in Streptococcus faecalis, a genetic analysis of the sex pheromone-dependent tetracycline resistance plasmid pCF-10 was initiated. Rare transconjugants obtained from short matings with wild-type donors not exposed to sex pheromones were screened for increased donor potential in a ...
G, Dunny, M, Yuhasz, E, Ehrenfeld
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Genetic organization of the bacterial conjugative transposon Tn916 [PDF]
Journal of Bacteriology, 1988 Tn916, which encodes resistance to tetracycline, is a 16.4-kilobase conjugative transposon originally identified on the chromosome of Streptococcus faecalis DS16. The transposon has been cloned in Escherichia coli on plasmid vectors, where it expresses tetracycline resistance; it can be reintroduced into S. faecalis via protoplast transformation.
E, Senghas +4 more
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Oligonucleotide-conjugated beads for transdominant genetic experiments [PDF]
Nucleic Acids Research, 2000 Transdominant genetics using expression libraries can identify proteins and peptides that affect cell division. In conjunction with these libraries, oligo-nucleotide-conjugated beads and flow cytometry were used to test a strategy that potentially expands the range of such genetic studies.
M J, Feldhaus +4 more
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Protein conjugation with genetically encoded unnatural amino acids [PDF]
Current Opinion in Chemical Biology, 2013 The site-specific incorporation of unnatural amino acids with orthogonal chemical reactivity into proteins enables the synthesis of structurally defined protein conjugates. Amino acids containing ketone, azide, alkyne, alkene, and tetrazine side chains can be genetically encoded in response to nonsense and frameshift codons.
Chan Hyuk, Kim +2 more
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Crosstalk Between Type VI Secretion System and Mobile Genetic Elements
Frontiers in Molecular Biosciences, 2019 Many bacterial processes require cell-cell contacts. Such are the cases of bacterial conjugation, one of the main horizontal gene transfer mechanisms that physically spreads DNA, and the type VI secretion systems (T6SSs), which deploy antibacterial ...
Arancha Peñil-Celis +1 more
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The Effect of Outer Space and Other Environmental Cues on Bacterial Conjugation
Microbiology Spectrum, 2023 Bacterial conjugation is one of the most abundant horizontal gene transfer (HGT) mechanisms, playing a fundamental role in prokaryote evolution. A better understanding of bacterial conjugation and its cross talk with the environment is needed for a more ...
Bar Piscon +9 more
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BMC Microbiology, 2020 Background Extended spectrum beta-lactamase (ESBL)-producing enterobacteria pose a major hazard to public health. Due to the possibility of genetic transfer, ESBL genes might spread to pathogenic enterobacterial strains.
Eva-Maria Saliu +2 more
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