Bacterial Conjugation Under Anaerobic Conditions [PDF]
Journal of Bacteriology, 1972 Chromosome transfer in Escherichia coli has been shown to occur at high frequencies in an anaerobic environment.
Donald R. Stallions, Roy Curtiss
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Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri. [PDF]
J Vis Exp, 2017 Transposon mutagenesis is a method that allows gene disruption via the random genomic insertion of a piece of DNA called a transposon. The protocol below outlines a method for high efficiency transfer between bacterial strains of a plasmid harboring a ...
Freed NE.
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Designer diatom episomes delivered by bacterial conjugation. [PDF]
Nat Commun, 2015 Eukaryotic microalgae hold great promise for the bioproduction of fuels and higher value chemicals. However, compared with model genetic organisms such as Escherichia coli and Saccharomyces cerevisiae, characterization of the complex biology and ...
Karas BJ +19 more
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Plasmids pick a bacterial partner before committing to conjugation [PDF]
Nucleic Acids Research, 2023 Bacterial conjugation was first described by Lederberg and Tatum in the 1940s following the discovery of the F plasmid. During conjugation a plasmid is transferred unidirectionally from one bacterium (the donor) to another (the recipient), in a contact ...
Gad Frankel +5 more
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Role of Pili in Bacterial Conjugation [PDF]
Journal of Bacteriology, 1970 We describe techniques for isolating individual pairs of mating Escherichia coli and observing them under the light microscope. Some pairs achieved close cell-to-cell contact, whereas others remained loosely connected by invisible connections which may be F pili.
Jonathan T. Ou, Thomas F. Anderson
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Conjugation of Carboxylated Graphene Quantum Dots with Cecropin P1 for Bacterial Biosensing Applications [PDF]
ACS Omega, 2020 Biosensors that can accurately and rapidly detect bacterial concentrations in solution are important for potential applications such as assessing drinking water safety.
Jonathan Bruce, Jude Clapper
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Bacterial conjugation: Running rings around DNA [PDF]
Current Biology, 2001 Helicases are active in many aspects of DNA replication, recombination, repair and transcription. An integral membrane bacterial protein assembly involved in the transfer of DNA between cells has been shown to resemble a ring helicase, suggesting that it hydrolyzes ATP to pump DNA through a central channel.
Edward H. Egelman
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Bacterial Conjugation in Rhodobacter capsulatus
Bio-Protocol, 2013 Bacterial conjugation of plasmids is the common method of introducing foreign DNA into Rhodobacter capsulatus because transformational systems have not been shown as efficient methods of introducing DNA to R. capsulatus. For R.
Molly Leung, John Beatty
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Expression of a novel class of bacterial Ig-like proteins is required for IncHI plasmid conjugation
PLoS Genetics, 2019 Antimicrobial resistance (AMR) is currently one of the most important challenges to the treatment of bacterial infections. A critical issue to combat AMR is to restrict its spread.
Mário Hüttener +9 more
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Specific gene repression by CRISPRi system transferred through bacterial conjugation. [PDF]
ACS Synth Biol, 2014 In microbial communities, bacterial populations are commonly controlled using indiscriminate, broad range antibiotics. There are few ways to target specific strains effectively without disrupting the entire microbiome and local environment.
Ji W +17 more
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