Results 121 to 130 of about 1,245,198 (329)
TTT and R2TP chaperone complexes are required for the assembly and activation of mTORC1. WAC directly interacts with components of TTT, R2TP, and mTORC1, and these interactions are affected by the availability of glucose and glutamine, correlating with changes in mTORC1 activity.
Sofía Cabezudo+11 more
wiley +1 more source
DNA extraction: High yield is not synonymous with high diversity
The quality, amount, and, in the case of polymicrobial samples, diversity of isolated DNA presupposes the precision of downstream application results. As part of a larger study on colorectal cancer, the aim of this work was to identify the DNA extraction
John Christopher Noone+2 more
doaj
Background Bacteria associated with insects can have a substantial impact on the biology and life cycle of their host. The checkerboard DNA-DNA hybridization technique is a semi-quantitative technique that has been previously employed in odontology to ...
Gaio Analiz de Oliveira+6 more
doaj +1 more source
Molecular mechanisms of autoimmunity triggered by microbial infection [PDF]
Autoimmunity can be triggered by microbial infection. In this context, the discovery of Toll-like receptors (TLRs) provides new insights and research perspectives.
Anders, Hans-Joachim+3 more
core +2 more sources
The regenerative potential of adipose tissue‐derived stem cells can be enhanced through chemical stimulation in vitro. A short stimulation protocol using forskolin, either alone or in combination with other growth factors, under xeno‐free conditions enhanced the pro‐angiogenic responses in human ASCs.
Maria Vittoria Giraudo+5 more
wiley +1 more source
Intravesicle Isothermal DNA Replication
Background Bacterial and viral DNA replication was previously reconstituted in vitro from component parts 1234. Significant advances in building minimal cell-like structures also have been made recently 567.
Ross Lindsey A+4 more
doaj +1 more source
16S rRNA gene-based profiling of the human infant gut microbiota is strongly influenced by sample processing and PCR primer choice [PDF]
Acknowledgements The authors acknowledge the assistance of Grietje Holtrop (RINH-BioSS) with the statistical analysis of the data and the Wellcome Trust Sanger Institute’s 454 pyrosequencing team for generating 16S rRNA gene data. AWW, PS and JP received
Flint, Harry J.+5 more
core +2 more sources
The glomerular filtration barrier (GFB) is the first step of blood filtration by the kidneys. The concerning increase of kidney diseases makes the development of new models essential. In this context, microphysiological glomerular filtration barriers focus on closely reproducing the physiological architecture of the in vivo GFB: podocytes, glomerular ...
Manon Miran+5 more
wiley +1 more source
Toll Meets Bacterial CpG-DNA [PDF]
While this minireview was in process, Hayashi et al. reported that bacterial flagellin signals via TLR5. Hayashi, F., Smith, K.D., Ozinsky, A., Hawn, T.R., Yi, E.C., Goodlett, D.R., Eng, J.K., Akira, S., Underhill, D.M., and Aderem, A. (2001). Nature 410, 1099–1103.
openaire +3 more sources
alphabeta sequence of F is IS31 [PDF]
Previous studies have shown that there is a deoxyribonucleic acid (DNA) segment, of length 1.3 kb and denoted as the alphabeta sequence, which occurs twice on the F plasmid at corrdinates 93.2 to 94.5/OF kb and 13.7 to 15.0F kb.
Cohen, Stanley N.+3 more
core