Results 11 to 20 of about 1,235,601 (229)

Bacterial DNA ligases [PDF]

open access: yesMolecular Microbiology, 2001
DNA ligases join breaks in the phosphodiester backbone of DNA molecules and are used in many essential reactions within the cell. All DNA ligases follow the same reaction mechanism, but they may use either ATP or NAD+ as a cofactor. All Bacteria (eubacteria) contain NAD+‐dependent DNA ligases, and the uniqueness of these enzymes to Bacteria makes them ...
Wilkinson, A, Day, J, Bowater, R
openaire   +4 more sources

Ribonucleotides in bacterial DNA [PDF]

open access: yesCritical Reviews in Biochemistry and Molecular Biology, 2014
In all living cells, DNA is the storage medium for genetic information. Being quite stable, DNA is well-suited for its role in storage and propagation of information, but RNA is also covalently included in DNA through various mechanisms. Recent studies also demonstrate useful aspects of including ribonucleotides in the genome during repair.
Justin R. Randall   +3 more
openaire   +3 more sources

Genomic and transcriptomic analysis of Ligilactobacillus salivarius IBB3154—in search of new promoters for vaccine construction

open access: yesMicrobiology Spectrum, 2023
Transcriptomic analysis of the genome sequenced Ligilactobacillus salivarius strain IBB3154 grown at two different temperatures (37°C vs 42°C) identified differentially expressed genes involved in metabolic pathways, osmoregulation, and surface protein ...
Patrycja Kobierecka   +6 more
doaj   +1 more source

Structural and mutagenic analysis of the RM controller protein C.Esp1396I [PDF]

open access: yes, 2014
Bacterial restriction-modification (RM) systems are comprised of two complementary enzymatic activities that prevent the establishment of foreign DNA in a bacterial cell: DNA methylation and DNA restriction.
Kneale, Geoff   +2 more
core   +15 more sources

Bacterial DNA in the diagnosis of spontaneous bacterial peritonitis [PDF]

open access: yesAlimentary Pharmacology & Therapeutics, 2010
Despite inoculation into blood culture bottles, ascitic fluid culture is negative in 50% of cases of spontaneous bacterial peritonitis (SBP).To determine whether 16S rDNA gene detection by real-time polymerase chain reaction (PCR) and sequencing increases the efficacy of culture in microbiological diagnosis of spontaneous bacterial peritonitis.We ...
Michel Montemayor   +15 more
openaire   +4 more sources

Organization of DNA in a bacterial nucleoid [PDF]

open access: yesBMC Microbiology, 2016
It is unclear how DNA is packaged in a bacterial cell in the absence of nucleosomes. To investigate the initial level of DNA condensation in bacterial nucleoid we used in vivo DNA digestion coupled with high-throughput sequencing of the digestion-resistant fragments. To this end, we transformed E.
Tolstorukov, Michael Y.   +3 more
openaire   +4 more sources

Circulating bacterial-derived DNA fragment level is a strong predictor of cardiovascular disease in peritoneal dialysis patients. [PDF]

open access: yesPLoS ONE, 2015
Circulating bacterial DNA fragment is related to systemic inflammatory state in peritoneal dialysis (PD) patients. We hypothesize that plasma bacterial DNA level predicts cardiovascular events in new PD patients.We measured plasma bacterial DNA level in ...
Cheuk-Chun Szeto   +11 more
doaj   +1 more source

Screening for Highly Transduced Genes in Staphylococcus aureus Revealed Both Lateral and Specialized Transduction

open access: yesMicrobiology Spectrum, 2022
Bacteriophage-mediated transduction of bacterial DNA is a major route of horizontal gene transfer in the human pathogen, Staphylococcus aureus. Transduction involves the packaging of bacterial DNA by viruses and enables the transmission of virulence and ...
Janine Zara Bowring   +5 more
doaj   +1 more source

The Influence of DNA Extraction and Lipid Removal on Human Milk Bacterial Profiles

open access: yesMethods and Protocols, 2020
Culture-independent molecular techniques have advanced the characterization of environmental and human samples including the human milk (HM) bacteriome. However, extraction of high-quality genomic DNA that is representative of the bacterial population in
Anna Ojo-Okunola   +6 more
doaj   +1 more source

Bacterial DNA is present in the fetal intestine and overlaps with that in the placenta in mice. [PDF]

open access: yesPLoS ONE, 2018
Bacterial DNA has been reported in the placenta and amniotic fluid by several independent groups of investigators. However, it's taxonomic overlap with fetal and maternal bacterial DNA in different sites has been poorly characterized. Here, we determined
Keith A Martinez   +13 more
doaj   +1 more source

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