Results 11 to 20 of about 1,273,913 (331)

Optimizing bacterial DNA extraction in urine.

PLoS ONE, 2019
Urine is an acceptable, non-invasive sample for investigating the human urogenital microbiota and for the diagnosis of sexually transmitted infections.
Matthew M Munch, Laura C Chambers, Lisa E Manhart, Dan Domogala, Anthony Lopez, David N Fredricks, Sujatha Srinivasan   +6 more
doaj   +5 more sources

Is human DNA enough? - Potential for bacterial DNA [PDF]

Frontiers in Genetics, 2013
Human identification has played an important role in forensic science for the past two decades and it will continue to do so. However, there are certain types of traces, for example, low quality and low quantity of DNA, often associated with violent crimes, which cannot always be satisfactorily exploited by current techniques. So what is next?
Sarah Louise Leake
doaj   +4 more sources

Bacterial DNA ligases [PDF]

Molecular Microbiology, 2001
DNA ligases join breaks in the phosphodiester backbone of DNA molecules and are used in many essential reactions within the cell. All DNA ligases follow the same reaction mechanism, but they may use either ATP or NAD+ as a cofactor. All Bacteria (eubacteria) contain NAD+‐dependent DNA ligases, and the uniqueness of these enzymes to Bacteria makes them ...
Wilkinson, A, Day, J, Bowater, R
openaire   +4 more sources

Ribonucleotides in bacterial DNA [PDF]

Critical Reviews in Biochemistry and Molecular Biology, 2014
In all living cells, DNA is the storage medium for genetic information. Being quite stable, DNA is well-suited for its role in storage and propagation of information, but RNA is also covalently included in DNA through various mechanisms. Recent studies also demonstrate useful aspects of including ribonucleotides in the genome during repair.
Justin R. Randall, Jeremy W. Schroeder, Lyle A. Simmons, Lindsay A. Matthews   +3 more
openaire   +3 more sources

Bacterial DNA in the diagnosis of spontaneous bacterial peritonitis [PDF]

Alimentary Pharmacology & Therapeutics, 2010
Despite inoculation into blood culture bottles, ascitic fluid culture is negative in 50% of cases of spontaneous bacterial peritonitis (SBP).To determine whether 16S rDNA gene detection by real-time polymerase chain reaction (PCR) and sequencing increases the efficacy of culture in microbiological diagnosis of spontaneous bacterial peritonitis.We ...
Michel Montemayor, R. Pericas, Ferran Navarro, Germán Soriano, Germán Soriano, Xavier Torras, Xavier Torras, Eva Román, Eva Román, Carlos Guarner-Argente, Óscar Esparcia, Carlos Guarner, Carlos Guarner, Pere Coll, Pere Coll, N. Calvo   +15 more
openaire   +4 more sources

Organization of DNA in a bacterial nucleoid [PDF]

BMC Microbiology, 2016
It is unclear how DNA is packaged in a bacterial cell in the absence of nucleosomes. To investigate the initial level of DNA condensation in bacterial nucleoid we used in vivo DNA digestion coupled with high-throughput sequencing of the digestion-resistant fragments. To this end, we transformed E.
Tolstorukov, Michael Y., Virnik, Konstantin, Zhurkin, Victor B., Adhya, Sankar   +3 more
openaire   +4 more sources

The Influence of DNA Extraction and Lipid Removal on Human Milk Bacterial Profiles

Methods and Protocols, 2020
Culture-independent molecular techniques have advanced the characterization of environmental and human samples including the human milk (HM) bacteriome. However, extraction of high-quality genomic DNA that is representative of the bacterial population in
Anna Ojo-Okunola, Shantelle Claassen-Weitz, Kilaza S. Mwaikono, Sugnet Gardner-Lubbe, Heather J. Zar, Mark P. Nicol, Elloise du Toit   +6 more
doaj   +1 more source

Presence of multiple bacterial markers in clinical samples might be useful for presumptive diagnosis of infection in cirrhotic patients with culture-negative reports [PDF]

, 2016
Bacterial infections in cirrhotic patients with ascites are associated with a severe prognosis and an increased risk of death. The microbiological standard tests for the diagnosis of suspected infection, based on culture test of blood and ascitic fluid ...
Boaretti, M, Castellani, F, Lleo, M. M., Lucidi, Cristina, Merli, Manuela   +4 more
core   +1 more source

Narrative Review of Bacterial DNA Extraction: Key Points and Challenges [PDF]

SINAI International Scientific Journal
Extraction of bacterial DNA is a critical step in obtaining high-quality DNA. The overall quality, yield, and integrity of the genomic DNA isolated affects the success of any downstream applications.
Samar Solyman, Manar El Samak
doaj   +1 more source

Circulating bacterial DNA in cardiovascular disease. [PDF]

Front Cardiovasc Med
Cardiovascular disease (CVD) remains a global health burden despite advances in prevention and treatment. Conventional biomarkers, while effective for a number of patient groups, fail to provide personalized diagnosis and prognosis, necessitating the exploration of novel markers. Advancements in sequencing technology have unveiled the role of cell-free
Appleby S, Purcell R.
europepmc   +4 more sources