Results 11 to 20 of about 854,281 (316)

The Influence of DNA Extraction and Lipid Removal on Human Milk Bacterial Profiles

Methods and Protocols, 2020
Culture-independent molecular techniques have advanced the characterization of environmental and human samples including the human milk (HM) bacteriome. However, extraction of high-quality genomic DNA that is representative of the bacterial population in
Anna Ojo-Okunola, Shantelle Claassen-Weitz, Kilaza S. Mwaikono, Sugnet Gardner-Lubbe, Heather J. Zar, Mark P. Nicol, Elloise du Toit   +6 more
doaj   +1 more source

Screening for Highly Transduced Genes in Staphylococcus aureus Revealed Both Lateral and Specialized Transduction

Microbiology Spectrum, 2022
Bacteriophage-mediated transduction of bacterial DNA is a major route of horizontal gene transfer in the human pathogen, Staphylococcus aureus. Transduction involves the packaging of bacterial DNA by viruses and enables the transmission of virulence and ...
Janine Zara Bowring, Yue Su, Ahlam Alsaadi, Sine L. Svenningsen, Julian Parkhill, Hanne Ingmer   +5 more
doaj   +1 more source

Adenylylation of Gyrase and Topo IV by FicT Toxins Disrupts Bacterial DNA Topology [PDF]

, 2015
Toxin-antitoxin (TA) modules are ubiquitous molecular switches controlling bacterial growth via the release of toxins that inhibit cell proliferation.
Gerdes, K., Glatter, Timo, Dehio, Christoph, Alexander Harms, Goepfert, A., Scheu, P., Kenn Gerdes, Glatter, T. ; https://orcid.org/, Schirmer, T., Harms, Alexander, Christoph Dehio, Harms, A., Timo Glatter, Stanger, Frédéric Valentin, Gerdes, Kenn, Dehio, C., Schirmer, Tilman, Harms, Alexander; id_orcid, de Jong, I., de Jong, Imke Greet, Stanger, F., Tilman Schirmer, Patrick Daniel Scheu, Arnaud Goepfert, Scheu, Patrick Daniel, Frédéric Valentin Stanger, Imke Greet de Jong, Goepfert, Arnaud   +27 more
core   +1 more source

Adjuvant activity of Pasteurella multocida A strain, Pasteurella multocida B strain and Salmonella typhimurium bacterial DNA on cellular and humoral immunity responses against Pasteurella multocida specific strain infections in Balb/c mice

Asian Pacific Journal of Tropical Medicine, 2018
Objective: To evaluate the effects of Pasteurella multocida (P. multocida) vaccines on the expression and release of antibodies, interleukin (IL)-6 and IL-12 by serum.
Maryam Homayoon, Yahya Tahamtan, Mohammad Kargar, Seyed Mohammad Hossein Hosseini, Abbas Akhavan Sepahy   +4 more
doaj   +1 more source

Segmenting bacterial and viral DNA sequence alignments with a trans-dimensional phylogenetic factorial hidden Markov model [PDF]

, 2009
The traditional approach to phylogenetic inference assumes that a single phylogenetic tree can represent the relationships and divergence between the taxa. However, taxa sequences exhibit varying levels of conservation, e.g.
Husmeier, D., Dirk Husmeier, Wolfgang P. Lehrach, Lehrach, W.P.   +3 more
core   +1 more source

Narrative Review of Bacterial DNA Extraction: Key Points and Challenges [PDF]

SINAI International Scientific Journal
Extraction of bacterial DNA is a critical step in obtaining high-quality DNA. The overall quality, yield, and integrity of the genomic DNA isolated affects the success of any downstream applications.
Samar Solyman, Manar El Samak
doaj   +1 more source

Identification of a Contact-Dependent Growth Inhibition (CDI) System That Reduces Biofilm Formation and Host Cell Adhesion of Acinetobacter baumannii DSM30011 Strain

Frontiers in Microbiology, 2019
Acinetobacter baumannii is a multidrug-resistant nosocomial opportunistic pathogen that is becoming a major health threat worldwide. In this study, we have focused on the A.
Morgane Roussin, Sedera Rabarioelina, Laurence Cluzeau, Julien Cayron, Christian Lesterlin, Suzana P. Salcedo, Sarah Bigot, Sarah Bigot   +7 more
doaj   +1 more source

Bacterial DNA is present in the fetal intestine and overlaps with that in the placenta in mice. [PDF]

PLoS ONE, 2018
Bacterial DNA has been reported in the placenta and amniotic fluid by several independent groups of investigators. However, it's taxonomic overlap with fetal and maternal bacterial DNA in different sites has been poorly characterized. Here, we determined
Keith A Martinez, Joann Romano-Keeler, Joseph P Zackular, Daniel J Moore, Robert M Brucker, Christopher Hooper, Shufang Meng, Naoko Brown, Simon Mallal, Jeff Reese, David M Aronoff, Hakdong Shin, Maria Gloria Dominguez-Bello, Jörn-Hendrik Weitkamp   +13 more
doaj   +1 more source

Plasmids of Psychrotolerant Polaromonas spp. Isolated From Arctic and Antarctic Glaciers – Diversity and Role in Adaptation to Polar Environments

Frontiers in Microbiology, 2018
Cold-active bacteria of the genus Polaromonas (class Betaproteobacteria) are important components of glacial microbiomes. In this study, extrachromosomal replicons of 26 psychrotolerant Polaromonas strains, isolated from Arctic and Antarctic glaciers ...
Anna Ciok, Karol Budzik, Marek K. Zdanowski, Jan Gawor, Jakub Grzesiak, Przemyslaw Decewicz, Robert Gromadka, Dariusz Bartosik, Lukasz Dziewit   +8 more
doaj   +1 more source

STRUCTURE AND IMMUNE ADJUVANT PROPERTIES OF CPG-D

Медицинская иммунология, 2014
Bacterial DNA, along with other bacterial pathogen-associated molecular structures, may activate both innate and adaptive immune systems. This activity is associated with by nonmethylated CpG dinucleotides, which are present in the bDNA molecule, and it ...
V. S. Polovinkina, E. Yu. Markov
doaj   +1 more source