Results 21 to 30 of about 1,273,913 (331)

Optimization of DNA extraction from human urinary samples for mycobiome community profiling. [PDF]

, 2019
IntroductionRecent data suggest the urinary tract hosts a microbial community of varying composition, even in the absence of infection. Culture-independent methodologies, such as next-generation sequencing of conserved ribosomal DNA sequences, provide an
Ackerman, A Lenore, Ackerman, James E, Anger, Jennifer Tash, Freeman, Michael R, Khalique, Muhammad Umair, Kim, Jayoung, NIH Multidisciplinary Approach to the Study of Chronic Pelvic Pain (MAPP), Tang, Jie, Underhill, David M   +8 more
core   +3 more sources

Adjuvant activity of Pasteurella multocida A strain, Pasteurella multocida B strain and Salmonella typhimurium bacterial DNA on cellular and humoral immunity responses against Pasteurella multocida specific strain infections in Balb/c mice

Asian Pacific Journal of Tropical Medicine, 2018
Objective: To evaluate the effects of Pasteurella multocida (P. multocida) vaccines on the expression and release of antibodies, interleukin (IL)-6 and IL-12 by serum.
Maryam Homayoon, Yahya Tahamtan, Mohammad Kargar, Seyed Mohammad Hossein Hosseini, Abbas Akhavan Sepahy   +4 more
doaj   +1 more source

Bacterial DNA is present in the fetal intestine and overlaps with that in the placenta in mice. [PDF]

PLoS ONE, 2018
Bacterial DNA has been reported in the placenta and amniotic fluid by several independent groups of investigators. However, it's taxonomic overlap with fetal and maternal bacterial DNA in different sites has been poorly characterized. Here, we determined
Keith A Martinez, Joann Romano-Keeler, Joseph P Zackular, Daniel J Moore, Robert M Brucker, Christopher Hooper, Shufang Meng, Naoko Brown, Simon Mallal, Jeff Reese, David M Aronoff, Hakdong Shin, Maria Gloria Dominguez-Bello, Jörn-Hendrik Weitkamp   +13 more
doaj   +1 more source

Remarkable stability of an instability-prone lentiviral vector plasmid in Escherichia coli Stbl3 [PDF]

, 2012
Large-scale production of plasmid DNA to prepare therapeutic gene vectors or DNA-based vaccines requires a suitable bacterial host, which can stably maintain the plasmid DNA during industrial cultivation.
Al-Allaf, FA, Mehmet, H, Tchetchelnitski, V, Tolmachov, OE, Zambetti, LP   +4 more
core   +1 more source

Comparison of five assays for DNA extraction from bacterial cells in human faecal samples [PDF]

, 2020
Aim To determine the most effective DNA extraction method for bacteria in faecal samples. Materials and Results This study assessed five commercial methods, that is, NucliSens easyMag, QIAamp DNA Stool Mini kit, PureLink Microbiome DNA purification kit ...
Glorieux, Griet, Gryp, Tessa, Joossens, M., Vaneechoutte, Mario   +3 more
core   +1 more source

Identification of a Contact-Dependent Growth Inhibition (CDI) System That Reduces Biofilm Formation and Host Cell Adhesion of Acinetobacter baumannii DSM30011 Strain

Frontiers in Microbiology, 2019
Acinetobacter baumannii is a multidrug-resistant nosocomial opportunistic pathogen that is becoming a major health threat worldwide. In this study, we have focused on the A.
Morgane Roussin, Sedera Rabarioelina, Laurence Cluzeau, Julien Cayron, Christian Lesterlin, Suzana P. Salcedo, Sarah Bigot, Sarah Bigot   +7 more
doaj   +1 more source

Plasmids of Psychrotolerant Polaromonas spp. Isolated From Arctic and Antarctic Glaciers – Diversity and Role in Adaptation to Polar Environments

Frontiers in Microbiology, 2018
Cold-active bacteria of the genus Polaromonas (class Betaproteobacteria) are important components of glacial microbiomes. In this study, extrachromosomal replicons of 26 psychrotolerant Polaromonas strains, isolated from Arctic and Antarctic glaciers ...
Anna Ciok, Karol Budzik, Marek K. Zdanowski, Jan Gawor, Jakub Grzesiak, Przemyslaw Decewicz, Robert Gromadka, Dariusz Bartosik, Lukasz Dziewit   +8 more
doaj   +1 more source

Choice of DNA extraction method affects detection of bacterial taxa from retail chicken breast

BMC Microbiology, 2022
Background Sequence-based methods for the detection of bacteria such as 16S rRNA amplicon sequencing and metagenomics can provide a comprehensive view of the bacterial microbiome of food.
Annika Flint, Anna Laidlaw, Leo Li, Courtney Raitt, Mary Rao, Ashley Cooper, Kelly Weedmark, Catherine Carrillo, Sandeep Tamber   +8 more
doaj   +1 more source

How endo- is endo-?: surface sterilization of delicate samples: a Bryopsis (Bryopsidales, Chlorophyta) case study [PDF]

, 2010
In the search for endosymbiotic bacteria, elimination of ectosymbionts is a key point of attention. Commonly, the surface of the host itself or the symbiotic structures are sterilized with aggressive substances such as chlorine or mercury derivatives ...
De Clerck, Olivier, Hollants, Joke, Leliaert, Frédérik, Willems, Anne   +3 more
core   +2 more sources

The bacterial capsule is a gatekeeper for mobile DNA

PLOS Biology, 2021
The horizontal transfer of mobile DNA is one of the signature moves of bacterial evolution, but the specific rules that govern this transfer remain elusive. In this PLOS Biology issue, Haudiquet and colleagues revealed that the interactions between mobile genetic elements and the bacterial capsule shape the horizontal flow of DNA in an important ...
Alfonso Santos-López, Jerónimo Rodríguez-Beltrán, Álvaro San Millán   +2 more
openaire   +6 more sources