Results 61 to 70 of about 1,273,913 (331)

Structural snapshots of Xer recombination reveal activation by synaptic complex remodeling and DNA bending [PDF]

, 2016
Bacterial Xer site-specific recombinases play an essential genome maintenance role by unlinking chromosome multimers, but their mechanism of action has remained structurally uncharacterized. Here, we present two high-resolution structures of Helicobacter
Adams, Afonine, Aihara, Arnold, Aussel, Barre, Barre, Biswas, Blakely, Blakely, Blakely, Britton, Carnoy, Chen, Cortez, Das, Debowski, Debowski, Diagne, Dong, Emsley, Ennifar, Fischer, Gopaul, Gopaul, Grainge, Grindley, Guo, Guo, Hendricks, Holliday, Jayaram, Jo, Kabsch, Karaca, Keller, Kelley, Kim, Krissinel, Kuempel, Le Bourgeois, Lee, Leroux, Li, Luscombe, May, McCoy, Midonet, Nagy, Nolivos, Nunes-Düby, Page, Pargellis, Pettersen, Pérals, Rother, Serre, Steiner, Subramanya, Terwilliger, Turan, Val, Van Duyne, Van Duyne, van Zundert, Vonrhein, Yang, Yang, Yates, Yates, Zawadzki, Zheng   +71 more
core   +4 more sources

The epithelial barrier theory proposes a comprehensive explanation for the origins of allergic and other chronic noncommunicable diseases

FEBS Letters, EarlyView.
Exposure to common noxious agents (1), including allergens, pollutants, and micro‐nanoplastics, can cause epithelial barrier damage (2) in our body's protective linings. This may trigger an immune response to our microbiome (3). The epithelial barrier theory explains how this process can lead to chronic noncommunicable diseases (4) affecting organs ...
Can Zeyneloglu, Huseyn Babayev, Ismail Ogulur, Sena Ardicli, Yagiz Pat, Duygu Yazici, Bingjie Zhao, Lihong Chang, Xiaoqing Liu, Paolo D'Avino, Manru Li, Ceren Biçer, Fatma Hacer Kurtoğlu Babayev, Raja Dhir, Kari C. Nadeau, Marie‐Charlotte Brüggen, Mubeccel Akdis, Cezmi A. Akdis   +17 more
wiley   +1 more source

Indirect DNA extraction method suitable for acidic soil with high clay content

MethodsX, 2018
DNA extraction is an essential procedure when investigating microbial communities in environmental samples by sequencing technologies. High clay soils can be problematic as DNA adsorbs to the clay particles and can thereby be preserved from lysed, non ...
Eva Högfors-Rönnholm, Stephan Christel, Sten Engblom, Mark Dopson   +3 more
doaj   +1 more source

Maturation of Pseudo-Nucleus Compartment in P. aeruginosa, Infected with Giant phiKZ Phage

Viruses, 2020
The giant phiKZ phage infection induces the appearance of a pseudo-nucleus inside the bacterial cytoplasm. Here, we used RT-PCR, fluorescent in situ hybridization (FISH), electron tomography, and analytical electron microscopy to study the morphology of ...
Yana A. Danilova, Viktoriia V. Belousova, Andrey V. Moiseenko, Innokentii E. Vishnyakov, Maria V. Yakunina, Olga S. Sokolova   +5 more
doaj   +1 more source

From omics to AI—mapping the pathogenic pathways in type 2 diabetes

FEBS Letters, EarlyView.
Integrating multi‐omics data with AI‐based modelling (unsupervised and supervised machine learning) identify optimal patient clusters, informing AI‐driven accurate risk stratification. Digital twins simulate individual trajectories in real time, guiding precision medicine by matching patients to targeted therapies.
Siobhán O'Sullivan, Lu Qi, Pierre Zalloua   +2 more
wiley   +1 more source

A Toll for lupus [PDF]

, 2005
Toll-like receptor (TLR)-9 recognizes CpG motifs in microbial DNA. TLR9 signalling stimulates innate antimicrobial immunity and modulates adaptive immune responses including autoimmunity against chromatin, e.g., in systemic lupus erythematosus (SLE ...
Anders HJ, H-J Anders, Stacey KJ
core   +1 more source

Thermostable neutral metalloprotease from Geobacillus sp. EA1 does not share thermolysin's preference for substrates with leucine at the P1′ position

FEBS Letters, EarlyView.
Knowing how proteases recognise preferred substrates facilitates matching proteases to applications. The S1′ pocket of protease EA1 directs cleavage to the N‐terminal side of hydrophobic residues, particularly leucine. The S1′ pocket of thermolysin differs from EA's at only one position (leucine in place of phenylalanine), which decreases cleavage ...
Grant R. Broomfield, Torsten Kleffmann, David J. Saul, Sigurd M. Wilbanks   +3 more
wiley   +1 more source

Control of bacterial DNA supercoiling [PDF]

Molecular Microbiology, 1992
SummaryTwo DNA topoisomerases control the level of negative supercoiling in bacterial cells. DNA gyrase introduces supercoils, and DNA topoisomerase I prevents super‐coiling from reaching unacceptably high levels. Perturbations of supercoiling are corrected by the substrate preferences of these topoisomerases with respect to DNA topology and by changes
openaire   +3 more sources

Incorporation characteristics of exogenous 15N-labeled thymidine, deoxyadenosine, deoxyguanosine and deoxycytidine into bacterial DNA.

PLoS ONE, 2020
Bacterial production has been often estimated from DNA synthesis rates by using tritium-labeled thymidine. Some bacteria species cannot incorporate extracellular thymidine into their DNA, suggesting their biomass production might be overlooked when using
Kenji Tsuchiya, Tomoharu Sano, Noriko Tomioka, Ayato Kohzu, Kazuhiro Komatsu, Ryuichiro Shinohara, Shinji Shimode, Tatsuki Toda, Akio Imai   +8 more
doaj   +1 more source

Is there a placental microbiota? A critical review and re-analysis of published placental microbiota datasets

BMC Microbiology, 2023
The existence of a placental microbiota is debated. The human placenta has historically been considered sterile and microbial colonization was associated with adverse pregnancy outcomes.
Jonathan J. Panzer, Roberto Romero, Jonathan M. Greenberg, Andrew D. Winters, Jose Galaz, Nardhy Gomez-Lopez, Kevin R. Theis   +6 more
doaj   +1 more source