Bacterial Transformation Buffers Environmental Fluctuations through the Reversible Integration of Mobile Genetic Elements [PDF]
mBio, 2020 Horizontal gene transfer (HGT) promotes the spread of genes within bacterial communities. Among the HGT mechanisms, natural transformation stands out as being encoded by the bacterial core genome.
Gabriel Carvalho +7 more
doaj +3 more sources
Bacterial Transformation of Aromatic Monomers in Softwood Black Liquor [PDF]
Frontiers in Microbiology, 2021 The valorization of lignin, a major component of plant-derived biomass, is essential to sustainable biorefining. We identified the major monoaromatic compounds present in black liquor, a lignin-rich stream generated in the kraft pulping process, and ...
Laura E. Navas +9 more
doaj +3 more sources
Synchronized Electromechanical Shock Wave-Induced Bacterial Transformation [PDF]
ACS Omega, 2019 We report a simple device that generates synchronized mechanical and electrical pressure waves for carrying out bacterial transformation. The mechanical pressure waves are produced by igniting a confined nanoenergetic composite material that provides ...
Rishi Kant +11 more
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Bacterial transformation of lignin: key enzymes and high-value products [PDF]
Biotechnology for Biofuels and BioproductsLignin, a natural organic polymer that is recyclable and inexpensive, serves as one of the most abundant green resources in nature. With the increasing consumption of fossil fuels and the deterioration of the environment, the development and utilization ...
Jinming Gu +8 more
doaj +3 more sources
Bacterial transformation v1 [PDF]
protocols.io, 2019 Transformation of competent Escherichia coli cultures with heterologous DNA. This protocol allows the introduction of external DNA into competent E. coli cells. Typically, the piece of DNA consists of a plasmid, either for its cloning or for heterologous protein expression.
Dana Mozaffari, Laura Kvedarauskaite
semanticscholar +7 more sources
Bacterial Transformation and Processing of Diatom-Derived Organic Matter: A Case Study for Skeletonema dohrnii. [PDF]
Front Microbiol, 2022 Bacterial transformation and processing of phytoplankton-derived organic matter are extremely important for the formation of ubiquitous organic matter (OM) in aquatic ecosystems.
Liu Y, Wang X, Sun J.
europepmc +2 more sources
Revisiting the Mechanisms Involved in Calcium Chloride Induced Bacterial Transformation [PDF]
Frontiers in Microbiology, 2017 Azka Asif +3 more
doaj +3 more sources
Non-antibiotic pharmaceuticals enhance the transmission of exogenous antibiotic resistance genes through bacterial transformation. [PDF]
ISME J, 2020 Antibiotic resistance is a serious global threat for public health. Considering the high abundance of cell-free DNA encoding antibiotic resistance genes (ARGs) in both clinical and environmental settings, natural transformation is an important horizontal
Wang Y +8 more
europepmc +2 more sources
Integrated FT-ICR MS and metabolome reveals diatom-derived organic matter by bacterial transformation under warming and acidification [PDF]
iScience, 2023 Summary: Bacterial transformation and processing of diatom-derived organic matter (OM) is extremely important for the cycling of production and energy in marine ecosystems; this process contributes to the production of microbial food webs. In this study,
Yang Liu, Chao Ma, Jun Sun
doaj +2 more sources
Altering under-represented DNA sequences elevates bacterial transformation efficiency [PDF]
mBio, 2023 A cornerstone of bacterial molecular biology is the ability to genetically manipulate the microbe under study. Many bacteria are difficult to manipulate genetically, a phenotype due in part to robust removal of newly acquired DNA, for example, by ...
Shuai Hu +5 more
doaj +2 more sources