Results 11 to 20 of about 1,940 (215)
Mechanisms of Transforming DNA Uptake to the Periplasm of Bacillus subtilis
mBio, 2021 Transformation is a widely distributed mechanism of bacterial horizontal gene transfer that plays a role in the spread of antibiotic resistance and virulence genes and more generally in evolution.
Jeanette Hahn +2 more
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A novel method for DNA delivery into bacteria using cationic copolymers [PDF]
Brazilian Journal of Medical and Biological Research, 2021 Amphiphilic copolymers have a wide variety of medical and biotechnological applications, including DNA transfection in eukaryotic cells. Still, no polymer-primed transfection of prokaryotic cells has been described.
V.V. de Souza +4 more
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Altering under-represented DNA sequences elevates bacterial transformation efficiency
mBio, 2023 A cornerstone of bacterial molecular biology is the ability to genetically manipulate the microbe under study. Many bacteria are difficult to manipulate genetically, a phenotype due in part to robust removal of newly acquired DNA, for example, by ...
Shuai Hu +5 more
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Bacterial Inactivation of Transforming Deoxyribonucleate [PDF]
Journal of Bacteriology, 1971 Competent and noncompetent bacteria are able to bind deoxyribonucleate (DNA). The consequence of this binding is different for the two types of bacteria. Competent bacteria are able to utilize the DNA for transformation. DNA exposed to noncompetent bacteria loses its biological activity with a coincident reduction in the double-stranded molecular ...
F P, Haseltine, M S, Fox
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Isolation, Cloning, and Heterologous Maintenance of a Class III Peroxidase Gene from Solanum tuberosum and Its Implications for Plant Defense Mechanisms [PDF]
BIO Web of ConferencesPlant growth, development, and defense reactions are greatly dependent on the massive multigene family of heme- containing glycoproteins called class III plant peroxidases (EC 1.11.1.7).
Monika +4 more
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Ecological Indicators, 2022 Anthropogenic source of heavy metals in the estuary-coastal sediment could cause serious environmental concerns. Thus, much needs to be concerned about the linkages between bacterial community, microbial nitrogen processes and heavy metals accumulation ...
Caixia Wang +5 more
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Bacterial Transformation v1 [PDF]
, 2019 This protocol explains how to transform a plasmid DNA template into competent bacterial cells which are then cultivated. The DNA is then purified from the cells using a miniprep kit. The competent cells used in this protocol come from the NEB®10-beta Competent E. coli kit. This protocol was adapted from the one provided with the cells.
Dana Mozaffari, Laura Kvedarauskaite
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Frontiers in Microbiology, 2020 Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis is a rapid and reliable method for bacterial identification.
Jin Ling +13 more
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Frontiers in Microbiology, 2023 Natural transformation, or the uptake of naked DNA from the external milieu by bacteria, holds a unique place in the history of biology. This is both the beginning of the realization of the correct chemical nature of genes and the first technical step to
Vui Yin Seow +6 more
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The bacterial transformation of abietic acid [PDF]
Biochemical Journal, 1968 An Alcaligenes species, which was isolated from soil, can utilize abietic acid as its sole carbon source. During growth, the bacterium transforms abietic acid into 5α-hydroxyabietic acid (I, R=OH), a product considered to be 7β-hydroxy-13-isopropyl-8ξ-podocarp-13-en-15-oic acid (II, R=H) and a compound, C20H28O3, which is believed to be an epoxy-γ ...
B E, Cross, P L, Myers
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