Results 121 to 130 of about 129,578 (294)

Animal research in the UK: Regulation, implementation, welfare and development of new approach methodologies

Animal Models and Experimental Medicine, EarlyView.
Scientific research with animals in the UK is regulated by the Animals (Scientific Procedures) Act 1986 with the National Centre for the Replacement, Refinement and Reduction of Animals in Research providing support for best practice and facilitating development of new approach methodologies.
Ewan St. John Smith, James Bussell, Maggie Gentry, Elliot Lilley, Cathy Merry, Judy MacArthur Clark, William Reynolds   +6 more
wiley   +1 more source

The Efficiency of Bacterial Vaccines on Mortality during the 'Spanish' Influenza Pandemic of 1918-19. [PDF]

Soc Hist Med, 2023
Roth DT.
europepmc   +1 more source

Live bacterial vaccines – a review and identification of potential hazards

, 2006
The use of live bacteria to induce an immune response to itself or to a carried vaccine component is an attractive vaccine strategy. Advantages of live bacterial vaccines include their mimicry of a natural infection, intrinsic adjuvant properties and ...
Ann Detmer, Jacob Glenting, Glenting Jacob, Detmer Ann   +3 more
core   +1 more source

A METHOD FOR STANDARDIZING BACTERIAL VACCINES [PDF]

JAMA: The Journal of the American Medical Association, 1913
The following method is presented as a convenient means for determining approximately the amount of bacterial substance in suspensions used as vaccines. It consists in measuring the volume of the moist bacteria after centrifugalization in a tube with a graduated capillary tip.
openaire   +1 more source

Dose‐dependent pathogenicity and inflammatory imbalance in severely immunodeficient mice infected with mpox virus

Animal Models and Experimental Medicine, EarlyView.
This study establishes an NPG (NOD.Cg‐Prkdcscid Il2rgtm1/Vst) mouse model for severe monkeypox virus infection in immunocompromised hosts, demonstrating dose‐dependent disease severity and revealing that unbalanced pro‐inflammatory cytokine release drives pathogenesis, providing insights for therapeutic interventions. Abstract The global spread of mpox
Na Li, Ziqing Jia, Zhe Cong, Jianrong Ma, Jiahan Lu, Yongzhi Hou, Fei Wang, Jiasen Yang, Ting Chen, Jingjing Zhang, Dong Zhang, Jing Xue   +11 more
wiley   +1 more source

Identification and genotyping of Chikungunya virus using reverse transcription polymerase chain reaction and restriction fragment length polymorphism methods

Биопрепараты: Профилактика, диагностика, лечение
INTRODUCTION. Chikungunya virus (CHIKV) genotyping involves sequencing fractions of genes encoding E1, E2, and nsP1 proteins or the entire genome of the virus.
N. A. Netesova, M. A. Abdurashitov, T. G. Samartseva, O. V. Klimovich, A. S. Oksanich, Е. V. Otrashevskaia, G. M. Ignatyev   +6 more
doaj   +1 more source

Screening of bacterial molecules with antagonistic or adjuvant activity

, 2010
The Lipopolysaccharides (LPSs) are the major constituents of the outer membrane of Gram-negative bacteria and the interest in their chemical characterization is motivated by the close correlation existing between the structure and the biological activity
Fregolino, Eleonora
core  

Nonviral Gold Nanoparticle‐Mediated Delivery of CRISPR‐Cas9 Ribonucleoprotein and Long DNA Transgenes Into Primary Blood Cells

Advanced NanoBiomed Research, EarlyView.
CRISPR/Cas9 has revolutionized the field of gene therapy, but delivery remains an outstanding issue. We propose a nonviral gold‐nanoparticle platform for co‐delivery of CRISPR/Cas9 ribonucleoprotein and long 2.1 kilobase dsDNA transgene constructs. This CRISPR‐AuNP is inexpensive to produce and mediate gene editing and DNA delivery in T cells and CD34+
Rachel A. Cunningham, Karthikeya S. V. Gottimukkala, Daniel D. Lane, Katrina Poljakov, Patricia Lipson, Mark R. Enstrom, Alessandro Rizzi, Aude G. Chapuis, Jennifer E. Adair   +8 more
wiley   +1 more source

Comparison of Receptor‐Mediated Endocytosis and Its Application to Enhance DNA Transfection by TFAMoplex

Advanced NanoBiomed Research, EarlyView.
We developed an assay to distinguish cellular binding from internalization. Compatible with microscopy and high‐throughput screening, the method identifies ligand‐mediated uptake. Applying top candidates to a protein‐based DNA carrier enhanced transfection efficiency, providing a rational strategy to improve non‐viral gene delivery systems.
David Scherer, Steffen Honrath, Jonas Kurmann, Alexander Klipp, Finn Brigger, Jean‐Christophe Leroux   +5 more
wiley   +1 more source

Molecular genetic methods for quality control of inactivated vaccines using a Chikungunya virus model: vaccine strain identification and completeness of virus inactivation

Биопрепараты: Профилактика, диагностика, лечение
INTRODUCTION. The completeness of virus inactivation and the identity of the vaccine strain are essential parameters for the safety and quality of inactivated virus vaccines, which should be controlled during vaccine development and production. Currently,
A. S. Oksanich, T. G. Samartseva, K. V. Kaa, E. V. Otrashevskaia, A. G. Krasko, A. G. Laputina, N. A. Netesova, G. M. Ignatyev   +7 more
doaj   +1 more source