Results 111 to 120 of about 129,985 (296)

One‐step generation of heritable mitochondrial DNA multiplex‐engineered rats using DddA‐derived cytosine base editor

Animal Models and Experimental Medicine, EarlyView.
We established that mixed DdCBE microinjection is an efficient, heritable, and precise strategy for generating multiplex mtDNA mutant rats. This advancement significantly expands the utility of DdCBEs for mitochondrial disease modeling, providing a robust platform for exploring the pathogenic mechanisms of complex mtDNA mutations and developing ...
Xu Zhang, Keru Li, Lei Tan, Wei Chen, Shan Gao, Chenyang Liu, Shuo Pan, Jiayue He, Ning Liu, Gefan Wan, Wei Dong, Weining Kong, Bin Shen, Xiaolong Qi, Yuanwu Ma   +14 more
wiley   +1 more source

Engraftment of sheep splenic lymphocytes into NBSGW mice and application in Brucella infection

Animal Models and Experimental Medicine, EarlyView.
A sheep splenocyte‐reconstituted mouse model (Sh‐Spl mice) was established via intravenous injection of sheep splenic lymphocytes into immunodeficient mice to evaluate Brucella infection. Abstract Background The development of immune reconstituted xenograft mouse models has addressed the limitations imposed by interspecies immunological differences in ...
Xiaowei Wang, Shicheng Wan, Yanmin Guo, Hongyu Han, Fei Luo, Dongmei Jiang, Xiaorong Yang, Quanhai Pang, Haisheng Yu, Jinlian Hua   +9 more
wiley   +1 more source

Phenotypic and Functional Characterization of Human Memory T Cell Responses to Burkholderia pseudomallei [PDF]

, 2009
Copyright: © 2009 Tippayawat et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and ...
Direk Limmathurotsakul, Suwannasaen, D, Sharon J Peacock, Ganjana Lertmemongkolchai, Titball, Richard W., Helen S Atkins, Tippayawat, Patcharaporn, Richard W Titball, Saenwongsa, Wipawee, Duangchan Suwannasaen, Chetchotisakd, Ploenchan, Chetchotisakd, P, Wipawee Saenwongsa, Jirawan Mahawantung, Philip L Felgner, Bancroft, Gregory J., Saenwongsa, W, Titball, RW, Atkins, Helen S, Mahawantung, J, Limmathurotsakul, Direk, Peacock, Sharon J., Limmathurotsakul, D, Mahawantung, Jirawan, Gregory J Bancroft, Suwannasaen, Duangchan, Peacock, SJ, Tippayawat, P, Atkins, HS, Titball, Richard W, Felgner, Philip L., Lertmemongkolchai, Ganjana, Felgner, Philip L, Ploenchan Chetchotisakd, Felgner, PL, Bancroft, GJ, Atkins, Helen S., Lertmemongkolchai, G, Bancroft, Gregory J, Peacock, Sharon J, Patcharaporn Tippayawat   +40 more
core   +1 more source

A NOTE ON THE PREPARATION OF BACTERIAL VACCINES [PDF]

Journal of the American Medical Association, 1914
The work of Vaughan, his associates and others interested in protein poisons has established the relationship of these substances to local and general anaphylactic reactions. Some of the reactions following the injection of bacterial vaccines are undoubtedly due to extraneous medium proteins which have not been removed from the bacterial suspensions in
openaire   +1 more source

Nonviral Gold Nanoparticle‐Mediated Delivery of CRISPR‐Cas9 Ribonucleoprotein and Long DNA Transgenes Into Primary Blood Cells

Advanced NanoBiomed Research, EarlyView.
CRISPR/Cas9 has revolutionized the field of gene therapy, but delivery remains an outstanding issue. We propose a nonviral gold‐nanoparticle platform for co‐delivery of CRISPR/Cas9 ribonucleoprotein and long 2.1 kilobase dsDNA transgene constructs. This CRISPR‐AuNP is inexpensive to produce and mediate gene editing and DNA delivery in T cells and CD34+
Rachel A. Cunningham, Karthikeya S. V. Gottimukkala, Daniel D. Lane, Katrina Poljakov, Patricia Lipson, Mark R. Enstrom, Alessandro Rizzi, Aude G. Chapuis, Jennifer E. Adair   +8 more
wiley   +1 more source

The Efficiency of Bacterial Vaccines on Mortality during the 'Spanish' Influenza Pandemic of 1918-19. [PDF]

Soc Hist Med, 2023
Roth DT.
europepmc   +1 more source

Rewriting Tumor Immunity With Bacterial Membrane Vesicles: A Versatile and Potent Therapeutic Strategy

Advanced NanoBiomed Research, EarlyView.
Bacterial membrane vesicles (BMVs) emerge as promising platforms for cancer immunotherapy owing to their intrinsic adjuvant properties and tunable cargo delivery capabilities. Their ability to modulate the tumor microenvironment, enhance antitumor immune responses, and support personalized therapeutic strategies highlights their growing potential as ...
Md Sifat Rahi, Md Mijanur Rahman, Asma Talukder, Urooj Yousaf Virk, Jennifer C. Wilson, Nigel McMillan, Ming Q. Wei   +6 more
wiley   +1 more source

A METHOD FOR STANDARDIZING BACTERIAL VACCINES [PDF]

JAMA: The Journal of the American Medical Association, 1913
The following method is presented as a convenient means for determining approximately the amount of bacterial substance in suspensions used as vaccines. It consists in measuring the volume of the moist bacteria after centrifugalization in a tube with a graduated capillary tip.
openaire   +1 more source

Identification and genotyping of Chikungunya virus using reverse transcription polymerase chain reaction and restriction fragment length polymorphism methods

Биопрепараты: Профилактика, диагностика, лечение
INTRODUCTION. Chikungunya virus (CHIKV) genotyping involves sequencing fractions of genes encoding E1, E2, and nsP1 proteins or the entire genome of the virus.
N. A. Netesova, M. A. Abdurashitov, T. G. Samartseva, O. V. Klimovich, A. S. Oksanich, Е. V. Otrashevskaia, G. M. Ignatyev   +6 more
doaj   +1 more source

Comparison of Receptor‐Mediated Endocytosis and Its Application to Enhance DNA Transfection by TFAMoplex

Advanced NanoBiomed Research, EarlyView.
We developed an assay to distinguish cellular binding from internalization. Compatible with microscopy and high‐throughput screening, the method identifies ligand‐mediated uptake. Applying top candidates to a protein‐based DNA carrier enhanced transfection efficiency, providing a rational strategy to improve non‐viral gene delivery systems.
David Scherer, Steffen Honrath, Jonas Kurmann, Alexander Klipp, Finn Brigger, Jean‐Christophe Leroux   +5 more
wiley   +1 more source