Results 171 to 180 of about 259,182 (233)

Modifying Bacteriophage $\lambda$ with Recombineering

Methods in molecular biology, 2009
Recombineering is a recently developed method of in vivo genetic engineering used in Escherichia coli and other Gram-negative bacteria. Recombineering can be used to create single-base changes, small and large deletions, and small insertions in phage lambda as well as in bacterial chromosomes, plasmids, and bacterial artificial chromosomes (BACS). This
Lynn C, Thomason, Amos B, Oppenheim, Donald L, Court   +2 more
openaire   +3 more sources

Architecture of the bacteriophage lambda tail.

Structure, 2023
Bacteriophage lambda has a double-stranded DNA genome and a long, flexible, non-contractile tail encoded by a contiguous block of 11 genes downstream of the head genes. The tail allows host recognition and delivery of viral DNA from the head shell to the
Chang Wang, Jinsong Duan, Zhiwei Gu, Xiaofei Ge, J. Zeng, Jiawei Wang   +5 more
semanticscholar   +1 more source

Protein degradation in E. coli: The ion mutation and bacteriophage lambda N and cll protein stability

Cell, 1981
S. Gottesman, M. Gottesman, J. Shaw, M. Pearson   +3 more
semanticscholar   +3 more sources

Bacteriophage lambda preconnectors

Journal of Molecular Biology, 1984
Jarema Kochan, Jose L. Carrascosa, Helios Murialdo   +2 more
openaire   +2 more sources

Bacteriophage Lambda (_)

2001
A. D. Hershey
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Two repressors in bacteriophage lambda

Molecular and General Genetics MGG, 1973
The functions of anti-immunity and immunity are very similar: both repress the early functions acting on the same two operators, oL and oR. They differ only in their effect on the expression of the cI gene.
M, Hampacherová, E, Koutecká, Z, Neubauer   +2 more
openaire   +2 more sources

Regulatory Circuits of Bacteriophage Lambda

1981
Publisher Summary This chapter summarizes the regulatory systems of λ to point out that both of its developmental pathways follow finely tuned temporal control mechanisms far more sophisticated than those conceived in the past decades. The chapter also emphasizes the salient features of the complex processes, such as: how a multivalent operator makes
S L, Adhya, S, Garges, D F, Ward
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Novel bacteriophage lambda mutation affecting lambda head assembly

Journal of Virology, 1979
A novel phage lambda mutation, called dc10, which interferes with proper lambda head assembly has been isolated and characterized. Phage lambda carrying this mutation is (i) unable to form plaques at 30 or 37 degrees C but does so at 42 degrees C and (ii) unable to form plaques at 42 degrees C on pN-constitutive hosts.
C P, Georgopoulos, R, Bisig, M, Magazin, H, Eisen, D, Court   +4 more
openaire   +2 more sources

Bacteriophage Lambda-Based Expression Vectors

Molecular Biotechnology, 2001
Bacteriophage lambda has been in use as a cloning vector for over 25 years, and has been used extensively as an expression vector. The efficiency of packaging and infection, and the simplicity of plaque screening are advantages of lambda as a cloning vector. A number of ingenious modifications help overcome the disadvantages associated with its mode of
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Densities of transducing lambda bacteriophages

Journal of Molecular Biology, 1961
Phages of a lysate of an induced culture of bacteria lysogenic for λ when centrifuged in a CsCl density gradient form a narrow band having a maximum at a definite density. The rare galactose-transducing phages contained in such a lysate are shown here to form a much wider band (especially towards the lower densities) having no definite maximum.
openaire   +2 more sources