Results 131 to 140 of about 13,887 (173)
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Replication of bacteriophage M13
Journal of Molecular Biology, 1977The origin for bacteriophage M13 single-strand synthesis has been localized by two different experimental approaches: (1) localization of the discontinuity in late life-cycle RFII ‡ , one of the initial product molecules during single-strand synthesis, and (2) determination of a gradient of radioactive label in RFI pulselabeled during single-strand ...
Lorraine Lica, Dan S. Ray
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Cold Spring Harbor Protocols, 2006
A plaque of bacteriophage M13 derives from infection of a single bacterium by a single virus particle. The progeny particles infect neighboring bacteria, which, in turn, release another generation of daughter virus particles. If the bacteria are growing in semisolid medium (e.g., containing agar or agarose), then the diffusion of the progeny particles ...
Joseph, Sambrook, David W, Russell
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A plaque of bacteriophage M13 derives from infection of a single bacterium by a single virus particle. The progeny particles infect neighboring bacteria, which, in turn, release another generation of daughter virus particles. If the bacteria are growing in semisolid medium (e.g., containing agar or agarose), then the diffusion of the progeny particles ...
Joseph, Sambrook, David W, Russell
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Cold Spring Harbor Protocols, 2017
A plaque of bacteriophage M13 derives from infection of a single bacterium by a single virus particle. The progeny particles infect neighboring bacteria, which, in turn, release another generation of daughter virus particles. If the bacteria are growing in semisolid medium (e.g., containing agar or agarose), then the diffusion of the progeny particles ...
Michael R, Green, Joseph, Sambrook
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A plaque of bacteriophage M13 derives from infection of a single bacterium by a single virus particle. The progeny particles infect neighboring bacteria, which, in turn, release another generation of daughter virus particles. If the bacteria are growing in semisolid medium (e.g., containing agar or agarose), then the diffusion of the progeny particles ...
Michael R, Green, Joseph, Sambrook
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Growing Bacteriophage M13 in Liquid Culture
Cold Spring Harbor Protocols, 2017Stocks of bacteriophage M13 are usually grown in liquid culture. The infected bacteria do not lyse but, instead, grow at a slower than normal rate to form a dilute suspension. The inoculum of bacteriophage is almost always a freshly picked plaque or a suspension of bacteriophage particles obtained from a single plaque, as described here. Infected cells
Michael R, Green, Joseph, Sambrook
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M13 Bacteriophages as Bioreceptors in Biosensor Device
2019New recognition probes sensible, specific and robust is one of the major problems of biosensor assay. Detection biosensors has utilized antibodies or enzymes as bioreceptors; however, these have numerous disadvantages of limited binding sites and physico-chemical instabilities, can negatively affect capture and detection of target in diagnostic device.
De Plano, Laura M. +6 more
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Growing Bacteriophage M13 in Liquid Culture
Cold Spring Harbor Protocols, 2006Stocks of bacteriophage M13 are usually grown in liquid culture. The infected bacteria do not lyse but, instead, grow at a slower than normal rate to form a dilute suspension. The inoculum of bacteriophage is almost always a freshly picked plaque or a suspension of bacteriophage particles obtained from a single plaque, as described here. Infected cells
Joseph, Sambrook, David W, Russell
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Genetic control of bacteriophage M13 DNA synthesis
Journal of Molecular Biology, 1968Abstract In the filamentous single-stranded DNA phage M13, the DNA replicates via the three steps shown in Figure 1: first, conversion of the infecting single-stranded molecule to a double-stranded replicative form; then, production of a pool of RF † molecules; finally, synthesis of progeny single strands.
D, Pratt, W S, Erdahl
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M13 bacteriophage displaying disulfide-constrained microproteins
Gene, 1993A display-phage library (TN2), displaying an 18-residue peptide fused to coat protein III, represents a collection of up to 8.55 x 10(6) peptides encoded by only 1.68 x 10(7) DNA sequences. Each displayed peptide has two fixed cysteine residues (allowing disulfide formation) and six variegated residues, four between the cysteines and one either side of
M A, McLafferty +3 more
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Structural characterization of bacteriophage M13 solubilization by amphiphiles
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 2002The structural properties of bacteriophage M13 during disassembly were studied in different membrane model systems, composed of a homologue series of the detergents sodium octyl sulfate, sodium decyl sulfate, and sodium dodecyl sulfate. The structural changes during phage disruption were monitored by spin-labeled electron spin resonance (ESR) and ...
Stopar, D. +3 more
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A cascade mechanism of transcription in bacteriophage M13 DNA
Virology, 1978Abstract Transcription studies were performed on M13 DNA restriction fragments which encompass a single ϱ-independent termination site and one or more promoter sites. From the results of these studies it has been concluded that a cascade-like mechanism of transcription operates during the expression of the phage M13 genome.
L, Edens +2 more
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