Results 151 to 160 of about 14,201 (176)
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Cold Spring Harbor Protocols, 2017
A plaque of bacteriophage M13 derives from infection of a single bacterium by a single virus particle. The progeny particles infect neighboring bacteria, which, in turn, release another generation of daughter virus particles. If the bacteria are growing in semisolid medium (e.g., containing agar or agarose), then the diffusion of the progeny particles ...
Michael R, Green, Joseph, Sambrook
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A plaque of bacteriophage M13 derives from infection of a single bacterium by a single virus particle. The progeny particles infect neighboring bacteria, which, in turn, release another generation of daughter virus particles. If the bacteria are growing in semisolid medium (e.g., containing agar or agarose), then the diffusion of the progeny particles ...
Michael R, Green, Joseph, Sambrook
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Cold Spring Harbor Protocols, 2006
A plaque of bacteriophage M13 derives from infection of a single bacterium by a single virus particle. The progeny particles infect neighboring bacteria, which, in turn, release another generation of daughter virus particles. If the bacteria are growing in semisolid medium (e.g., containing agar or agarose), then the diffusion of the progeny particles ...
Joseph, Sambrook, David W, Russell
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A plaque of bacteriophage M13 derives from infection of a single bacterium by a single virus particle. The progeny particles infect neighboring bacteria, which, in turn, release another generation of daughter virus particles. If the bacteria are growing in semisolid medium (e.g., containing agar or agarose), then the diffusion of the progeny particles ...
Joseph, Sambrook, David W, Russell
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Growing Bacteriophage M13 in Liquid Culture
Cold Spring Harbor Protocols, 2017Stocks of bacteriophage M13 are usually grown in liquid culture. The infected bacteria do not lyse but, instead, grow at a slower than normal rate to form a dilute suspension. The inoculum of bacteriophage is almost always a freshly picked plaque or a suspension of bacteriophage particles obtained from a single plaque, as described here. Infected cells
Michael R, Green, Joseph, Sambrook
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Growing Bacteriophage M13 in Liquid Culture
Cold Spring Harbor Protocols, 2006Stocks of bacteriophage M13 are usually grown in liquid culture. The infected bacteria do not lyse but, instead, grow at a slower than normal rate to form a dilute suspension. The inoculum of bacteriophage is almost always a freshly picked plaque or a suspension of bacteriophage particles obtained from a single plaque, as described here. Infected cells
Joseph, Sambrook, David W, Russell
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M13 bacteriophage displaying disulfide-constrained microproteins
Gene, 1993A display-phage library (TN2), displaying an 18-residue peptide fused to coat protein III, represents a collection of up to 8.55 x 10(6) peptides encoded by only 1.68 x 10(7) DNA sequences. Each displayed peptide has two fixed cysteine residues (allowing disulfide formation) and six variegated residues, four between the cysteines and one either side of
M A, McLafferty +3 more
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Genetic control of bacteriophage M13 DNA synthesis
Journal of Molecular Biology, 1968Abstract In the filamentous single-stranded DNA phage M13, the DNA replicates via the three steps shown in Figure 1: first, conversion of the infecting single-stranded molecule to a double-stranded replicative form; then, production of a pool of RF † molecules; finally, synthesis of progeny single strands.
D, Pratt, W S, Erdahl
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M13 bacteriophage production for large-scale applications
Bioprocess and Biosystems Engineering, 2014Bacteriophage materials have the potential to revolutionize medicine, energy production and storage, agriculture, solar cells, optics and many other fields. To fulfill these needs, this study examined critical process parameters during phage propagation to increase phage production capability. A representative scale-down system was created in tube spin
Christopher M, Warner +3 more
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Structural characterization of bacteriophage M13 solubilization by amphiphiles
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 2002The structural properties of bacteriophage M13 during disassembly were studied in different membrane model systems, composed of a homologue series of the detergents sodium octyl sulfate, sodium decyl sulfate, and sodium dodecyl sulfate. The structural changes during phage disruption were monitored by spin-labeled electron spin resonance (ESR) and ...
Stopar, D. +3 more
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M13 Bacteriophages as Bioreceptors in Biosensor Device
2019New recognition probes sensible, specific and robust is one of the major problems of biosensor assay. Detection biosensors has utilized antibodies or enzymes as bioreceptors; however, these have numerous disadvantages of limited binding sites and physico-chemical instabilities, can negatively affect capture and detection of target in diagnostic device.
De Plano, Laura M. +6 more
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Cloning SV40 HindIII restriction fragments into M13 bacteriophage
Gene, 1982Abstract The six Hind III restriction fragments of the simian virus 40 (SV40) genome were cloned into the bacteriophage M13mp5 vector to generate strand-specific hybridization probes.
K, Dixon +3 more
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