Results 31 to 40 of about 47,591 (156)
Nucleic Acids Research, 1990 Gene 27 of bacteriophage T4 D encodes a structural component of the central hub of the phage base plate (1). This gene maps between coordinates 116.0 and 117.2 on the T4 map. We present here the complete sequence of gene 27, derived from several T4 inserts subcloned in pUC and pBR322 vectors from the original clone 656 (from T.Young).
Bova R. +8 more
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THYMINELESS MUTAGENESIS IN BACTERIOPHAGE T4 [PDF]
Genetics, 1973 ABSTRACT Thymine deprivation can be achieved in bacteriophage T4 either by the use of the thymidylate synthetase inhibitor FUdR, or by an appropriate combination of genetic blocks; both methods produce marked mutagenesis. Extensive tests of the specificity of thymineless mutagenesis reveal that only A:T base pairs are affected, and that ...
M D, Smith +3 more
openaire +2 more sources
A promiscuous DNA packaging machine from bacteriophage T4.
PLoS Biology, 2011 Complex viruses are assembled from simple protein subunits by sequential and irreversible assembly. During genome packaging in bacteriophages, a powerful molecular motor assembles at the special portal vertex of an empty prohead to initiate packaging ...
Zhihong Zhang +6 more
doaj +1 more source
Facile enzymatic synthesis of base J-containing oligodeoxyribonucleotides and an analysis of the impact of base J on DNA replication in cells. [PDF]
PLoS ONE, 2014 We reported here the use of T4 bacteriophage β-glucosyltransferase (T4 β-GT) for the facile synthesis of base J-containing oligodeoxyribonucleotides (ODNs). We found that the enzyme could catalyze the glucosylation of 5-hydroxymethyl-2-deoxyuridine (5hmU)
Debin Ji, Yinsheng Wang
doaj +1 more source
Mismatch repair in recombination of bacteriophage T4
Biomolecular Concepts, 2012 The review focuses on the mechanism of mismatch repair in bacteriophage T4. It was first observed in T4 as an extra recombination mechanism, which contributed to the general recombination only when particular rII mutations were used as genetic markers ...
Shcherbakov Victor P.
doaj +1 more source
Covalent Modification of Bacteriophage T4 DNA Inhibits CRISPR-Cas9
mBio, 2015 The genomic DNAs of tailed bacteriophages are commonly modified by the attachment of chemical groups. Some forms of DNA modification are known to protect phage DNA from cleavage by restriction enzymes, but others are of unknown function.
Alexandra L. Bryson +7 more
doaj +1 more source
Viruses, 2021 Bacteriophage-encoded endolysins have been identified as antibacterial candidates. However, the development of endolysins as mainstream antibacterial agents first requires a comprehensive biochemical understanding. This study defines the atomic structure
Michael J. Love +5 more
doaj +1 more source
CRISPR/Cas9-mediated phage resistance is not impeded by the DNA modifications of phage T4.
PLoS ONE, 2014 Bacteria rely on two known DNA-level defenses against their bacteriophage predators: restriction-modification and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR-associated (Cas) systems.
Stephanie J Yaung +2 more
doaj +1 more source
Engineering Microbial Particles for Next‐Generation Biomedical Platforms
Advanced Science, EarlyView.Microbe‐derived particles (MDPs), which include extracellular vesicles, outer membrane vesicles, inclusion bodies, polysaccharide particles, and virus‐like particles, represent a rapidly expanding category of bioinspired nanomaterials. With their natural origin, intrinsic biocompatibility, and highly programmable functionality, MDPs serve as a ...
Yuting Li +7 more
wiley +1 more source
Structure and Function of Bacteriophage T4 [PDF]
Future Microbiology, 2014 Bacteriophage T4 is the most well-studied member of Myoviridae, the most complex family of tailed phages. T4 assembly is divided into three independent pathways: the head, the tail and the long tail fibers. The prolate head encapsidates a 172 kbp concatemeric dsDNA genome.
Moh Lan, Yap, Michael G, Rossmann
openaire +2 more sources