Results 51 to 60 of about 47,591 (156)

A New Label-Free Technique for Analysing Evaporation Induced Self-Assembly of Viral Nanoparticles Based on Enhanced Dark-Field Optical Imaging

Nanomaterials, 2017
Nanoparticle self-assembly is a complex phenomenon, the control of which is complicated by the lack of appropriate tools and techniques for monitoring the phenomenon with adequate resolution in real-time.
Ima Ghaeli, Zeinab Hosseinidoust, Hooshiar Zolfagharnasab, Fernando Jorge Monteiro   +3 more
doaj   +1 more source

A viral genome packaging ring-ATPase is a flexibly coordinated pentamer

Nature Communications, 2021
In viruses, multi-subunit ring-ATPases are involved in genome packaging. Here, using single-molecule techniques, the authors determine that the active bacteriophage T4 DNA packaging motor is a pentamer and show that the motor can tolerate inactive ...
Li Dai, Digvijay Singh, Suoang Lu, Vishal I. Kottadiel, Reza Vafabakhsh, Marthandan Mahalingam, Yann R. Chemla, Taekjip Ha, Venigalla B. Rao   +8 more
doaj   +1 more source

Evolutionary insights into bilin biosynthesis: Functional characterization of pre‐PcyA enzymes

Protein Science, Volume 35, Issue 9, September 2026.
Abstract Biosynthesis of the linear tetrapyrrole phycocyanobilin (PCB) by the ferredoxin‐dependent bilin reductase (FDBR) PcyA is essential for light‐harvesting and regulatory processes in diverse photosynthetic organisms, yet its evolutionary origins are not fully understood. PcyA evolved from pre‐PcyA proteins found in diverse bacteria.
Federica Frascogna, Nathan C. Rockwell, Gunhild Layer, Nicole Frankenberg‐Dinkel   +3 more
wiley   +1 more source

T4 phage and its head surface proteins do not stimulate inflammatory mediator production. [PDF]

PLoS ONE, 2013
Viruses are potent activators of the signal pathways leading to increased cytokine or ROS production. The effects exerted on the immune system are usually mediated by viral proteins. Complementary to the progress in phage therapy practice, advancement of
Paulina Miernikiewicz, Krystyna Dąbrowska, Agnieszka Piotrowicz, Barbara Owczarek, Justyna Wojas-Turek, Jagoda Kicielińska, Joanna Rossowska, Elżbieta Pajtasz-Piasecka, Katarzyna Hodyra, Katarzyna Macegoniuk, Kamila Rzewucka, Agnieszka Kopciuch, Tomasz Majka, Andrey Letarov, Eugene Kulikov, Henryk Maciejewski, Andrzej Górski   +16 more
doaj   +1 more source

Advances in large DNA fragment assembly for microbial cell factory engineering

Quantitative Biology, Volume 14, Issue 3, September 2026.
Abstract The efficient, rapid, and reliable assembly of DNA fragments is essential for advancing metabolic engineering and synthetic biology. With the rapid advancement of DNA synthesis and assembly technologies, the scale of DNA assembly has expanded from single genes to metabolic pathways and even genomes.
Yu Zhang, Wenqian Liu, Dongyuan Cheng, Jiazhang Lian, Xian Fu, Yue Shen   +5 more
wiley   +1 more source

Writing Big in Plant Genomes: Advances, Challenges and Strategies for Targeted Large‐Fragment DNA Insertion

Plant, Cell &Environment, Volume 49, Issue 9, Page 6506-6520, September 2026.
ABSTRACT Precise genome editing has transformed plant biology and crop improvement by enabling targeted modification of endogenous loci. Beyond gene knockout and base editing, the site‐specific insertion of exogenous DNA, particularly large DNA fragments, has become a central goal for engineering complex traits, reconstructing metabolic pathways and ...
Fengfeng Zhang, Daqi Yan, Jiayi Hou, Dan Yang, Yan Xiong, Mingzhang Wen, Xiaoyue Zhu   +6 more
wiley   +1 more source

The effect of bacteriophages T4 and HAP1 on in vitro melanoma migration

BMC Microbiology, 2009
Background The antibacterial activity of bacteriophages has been described rather well. However, knowledge about the direct interactions of bacteriophages with mammalian organisms and their other, i.e.
Boratyński Janusz, Świtała-Jeleń Kinga, Żaczek Maciej, Owczarek Barbara, Wietrzyk Joanna, Kurzępa Aneta, Jończyk Paulina, Skaradziński Grzegorz, Dąbrowska Krystyna, Poźniak Gryzelda, Maciejewska Magdalena, Górski Andrzej   +11 more
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, Volume 16, Issue 8, Page 1459-1476, August 2026.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Transcription of the T4 late genes

Virology Journal, 2010
This article reviews the current state of understanding of the regulated transcription of the bacteriophage T4 late genes, with a focus on the underlying biochemical mechanisms, which turn out to be unique to the T4-related family of phages or ...
Kassavetis George A, Geiduschek E Peter
doaj   +1 more source

Structural basis of the T4 bacteriophage primosome assembly and primer synthesis

Nature Communications, 2023
The T4 bacteriophage gp41 helicase and gp61 primase assemble into a primosome to couple DNA unwinding with RNA primer synthesis for DNA replication. How the primosome is assembled and how the primer length is defined are unclear.
Xiang Feng, Michelle M. Spiering, Ruda de Luna Almeida Santos, Stephen J. Benkovic, Huilin Li   +4 more
doaj   +1 more source