Results 41 to 50 of about 190,483 (202)

Genetic analysis of bacteriophage T4 transducing bacteriophages [PDF]

Journal of Virology, 1982
Mutations in the genes for nuclear disruption (ndd), endonuclease IV (denB), and the D1 region of the T4 genome are essential for converting bacteriophage T4 into a generalized transducing phage. These mutations gave rise to a very low frequency of transduction, about 10(-8) per infected bacterium.
K K, Young, G J, Edlin, G G, Wilson
openaire   +2 more sources

Mismatch repair in recombination of bacteriophage T4

Biomolecular Concepts, 2012
The review focuses on the mechanism of mismatch repair in bacteriophage T4. It was first observed in T4 as an extra recombination mechanism, which contributed to the general recombination only when particular rII mutations were used as genetic markers ...
Shcherbakov Victor P.
doaj   +1 more source

Facile enzymatic synthesis of base J-containing oligodeoxyribonucleotides and an analysis of the impact of base J on DNA replication in cells. [PDF]

PLoS ONE, 2014
We reported here the use of T4 bacteriophage β-glucosyltransferase (T4 β-GT) for the facile synthesis of base J-containing oligodeoxyribonucleotides (ODNs). We found that the enzyme could catalyze the glucosylation of 5-hydroxymethyl-2-deoxyuridine (5hmU)
Debin Ji, Yinsheng Wang
doaj   +1 more source

The ecology of bacteriophage T4.

, 1990
In this dissertation I explore the ecology of bacteriophage T4, a virus of Escherichia coli. In particular, I argue that the life history of bacteriophage T4 can be divided into the growth and survival of T4 phages in three distinct environments. I argue
Abedon, Stephen Tobias.
core   +5 more sources

Covalent Modification of Bacteriophage T4 DNA Inhibits CRISPR-Cas9

mBio, 2015
The genomic DNAs of tailed bacteriophages are commonly modified by the attachment of chemical groups. Some forms of DNA modification are known to protect phage DNA from cleavage by restriction enzymes, but others are of unknown function.
Alexandra L. Bryson, Young Hwang, Scott Sherrill-Mix, Gary D. Wu, James D. Lewis, Lindsay Black, Tyson A. Clark, Frederic D. Bushman   +7 more
doaj   +1 more source

The Molecular Basis for Escherichia coli O157:H7 Phage FAHEc1 Endolysin Function and Protein Engineering to Increase Thermal Stability

Viruses, 2021
Bacteriophage-encoded endolysins have been identified as antibacterial candidates. However, the development of endolysins as mainstream antibacterial agents first requires a comprehensive biochemical understanding. This study defines the atomic structure
Michael J. Love, David Coombes, Sarah H. Manners, Gayan S. Abeysekera, Craig Billington, Renwick C. J. Dobson   +5 more
doaj   +1 more source

Experimental test of connector rotation during DNA packaging into bacteriophage phi29 capsids [PDF]

, 2007
The bacteriophage phi29 generates large forces to compact its double-stranded DNA genome into a protein capsid by means of a portal motor complex. Several mechanical models for the generation of these high forces by the motor complex predict coupling of ...
Hetherington, Craig L., Craig L Hetherington, Falk, Wayne, Wayne Falk, Thorsten Hugel, Jardine, Paul J., Falk Wayne, Hugel Thorsten, Bustamante, Carlos, Grimes Shelley, Michaelis, Jens, Walter Jessica M, Bustamante Carlos, Michaelis Jens, Anderson, Dwight L., Hugel, Thorsten, Grimes, Shelley, Paul J Jardine, Jessica M Walter, Walter, Jessica M., Jardine Paul J, Dwight L Anderson, Jens Michaelis, Anderson Dwight L, Carlos Bustamante, Hetherington Craig L, Shelley Grimes   +26 more
core   +1 more source

CRISPR/Cas9-mediated phage resistance is not impeded by the DNA modifications of phage T4.

PLoS ONE, 2014
Bacteria rely on two known DNA-level defenses against their bacteriophage predators: restriction-modification and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR-associated (Cas) systems.
Stephanie J Yaung, Kevin M Esvelt, George M Church   +2 more
doaj   +1 more source

Lab‐in‐a‐Tube System Integrating Hierarchically Wrinkled Electrodes and DNAzyme‐Functionalized Beads for Diagnosis of Clostridioides difficile Infection

Advanced Materials, EarlyView.
The point‐of‐care diagnosis of Clostridioides difficile infection remains challenging due to reliance on slow culture and amplification‐based methods. A lab‐in‐a‐tube electrochemical biosensor integrating DNAzymes, antifouling magnetic beads, and a hierarchically structured flow cell enables amplification‐ and culture‐free detection directly in stool ...
Survanshu Saxena, Soyeon Lee, Youngeun Choi, Jonathan L'Heureux‐Hache, Jiaying Liu, Sadman Sakib, Sara M. Imani, Marek Smieja, Yingfu Li, Todd Hoare, Leyla Soleymani   +10 more
wiley   +1 more source

THYMINELESS MUTAGENESIS IN BACTERIOPHAGE T4 [PDF]

Genetics, 1973
ABSTRACT Thymine deprivation can be achieved in bacteriophage T4 either by the use of the thymidylate synthetase inhibitor FUdR, or by an appropriate combination of genetic blocks; both methods produce marked mutagenesis. Extensive tests of the specificity of thymineless mutagenesis reveal that only A:T base pairs are affected, and that ...
M D, Smith, R R, Green, L S, Ripley, J W, Drake   +3 more
openaire   +2 more sources