Results 91 to 100 of about 9,873 (221)
Delipidation of bacteriorhodopsin and reconstitution with exogenous phospholipid. [PDF]
, 1980 Solubilizations of the purple membrane from Halobacterium halobium with the detergent Tritain X-100 followed by gel filtration in deoxycholate solution gave bacteriorhodopsin that was more than 99% free from endogenous lipid.
Khorana, HG, Huang, KS, Bayley, H
core +1 more source
Photoanisotropic incoherent to coherent converter using a bacteriorhodopsin thin film [PDF]
, 1995 We have fabricated a novel optically addressed spatial light modulator by using bacteriorhodopsin immobilized in a polymer thin film. This incoherent-to-coherent converter is based on photoinduced anisotropy, as distinguished from previously reported ...
Ramanujam, P.S., Lindvold, L., Imam, H.
core +1 more source
PhotochemTime-resolved (TR) resonance Raman (RR) spectroscopy with continuous-wave excitation is a fundamental technique that has contributed substantially to the understanding of the structure and dynamics of retinal proteins.
Anna Lena Schäfer +5 more
doaj +1 more source
Acta Crystallographica Section D, Volume 82, Issue 5, Page 504-515, May 2026.Radiation‐induced changes during X‐ray crystallographic data collection can compromise concurrent spectroscopic studies. Suitable protocols to detect and identify spectral artefacts from crystallization buffers and cryoprotectants are essential for the reliable analysis of chromophoric ligands in macromolecules.Online UV–Vis absorption spectroscopy is ...
Jack Stubbs +8 more
wiley +1 more source
Spatial Organization of Bacteriorhodopsin in Model Membranes [PDF]
, 2002 Bacteriorhodopsin is a proton-transporting membrane protein in Halophilic archaea, and it is considered a prototype of membrane transporters and a model for G-protein-coupled receptors.
Poolman, Bert, +7 more
core +1 more source
Light Color‐Controlled pH‐Adjustment of Aqueous Solutions Using Engineered Proteoliposomes
Advanced ScienceControlling the pH at the microliter scale can be useful for applications in research, medicine, and industry, and therefore represents a valuable application for synthetic biology and microfluidics.
Daniel Harder +4 more
doaj +1 more source
ChemPhysChem, Volume 27, Issue 7, 14 April 2026.The microbial retinal protein KR2 (Krokinobacter eikastus) is a light‐driven outward Na+ pump, which is coupled to the photocycle of the chromophore. Using time‐resolved continuous‐wave pump‐probe resonance Raman spectroscopy, the operational switches for ion transport are identified, which are the transitions to a specific Schiff base sub‐state and ...
Anna Lena Schäfer +5 more
wiley +1 more source
Bacteriorhodopsin Folds through a Poorly Organized Transition State [PDF]
, 2015 The folding mechanisms of helical membrane proteins remain largely uncharted. Here we characterize the kinetics of bacteriorhodopsin folding and employ φ-value analysis to explore the folding transition state.
Chiwook Park (652907) +3 more
core +1 more source
Lipidic cubic phase serial millisecond crystallography using synchrotron radiation
IUCrJ, 2015 Lipidic cubic phases (LCPs) have emerged as successful matrixes for the crystallization of membrane proteins. Moreover, the viscous LCP also provides a highly effective delivery medium for serial femtosecond crystallography (SFX) at X-ray free-electron ...
Przemyslaw Nogly +26 more
doaj +1 more source
Sensors, 2018 Bacteriorhodopsin-embedded purple membranes (PM) have been demonstrated to be a sensitive photoelectric transducer for microbial detection. To efficiently prepare versatile BR-based immunosensors with protein A as antibody captures, a large, high ...
Hsueh-Hsia Wu +7 more
doaj +1 more source