Results 61 to 70 of about 9,873 (221)
Deep Mutational Scanning for the Study and Engineering of Protein Assemblies
This review explores how deep mutational scanning (DMS) can be used to improve understanding and accelerate engineering of protein assemblies, from natural fibers to engineered nanocages. We discuss various types of protein assemblies and their characterization, provide an introduction to the DMS technique, and then highlight examples in which DMS has ...
Jenna B. Wolfanger +2 more
wiley +1 more source
Photoactive bacteriorhodopsin variants [PDF]
Bacteriorhodopsin variants were generated by UV mutagenesis. The protein variants thus engineered were characterized for their UV-visible absorption, light induced proton release activity and photocycle kinetics (BR→M)
Singh, Anil K. +2 more
core +1 more source
In the purple membrane (PM) of Halobacterium salinarum is present a phospholipid dimer consisting of sulfo-triglycosyl-diether (S-TGD-1) esterified to the phosphate group of phosphatidic acid (PA), i.e., S-TGD-1-PA, called glycocardiolipin (GlyC ...
Simona Lobasso +3 more
doaj +1 more source
Decoding the Hot‐Mitochondrion Paradox
A distributed mechanism of mitochondrial heat generation is proposed in which all ion‐translocating proteins embedded within the inner mitochondrial membrane contribute to localized heat generation. All ion‐translocating proteins embedded within the inner mitochondrial membrane (IMM)—with ATP synthase shown here as an example—are proposed to function ...
Peyman Fahimi +2 more
wiley +1 more source
Large scale preparation of homogeneous bacteriorhodopsin [PDF]
Homogeneous bacteriorhodopsin was obtained preparatively (100 mg batches) from purple membrane of Halobacterium halobium cells. The homogeneity of the protein was considerably affected by variations in the growth conditions of the bacteria.
Bernard Lorber +3 more
core +1 more source
Scotty: lattice coincidences in the Protein Data Bank
Scotty supports crystallographic comparisons of lattice coincidences, the interpretation of oligomeric assemblies and paracrystallinity.The application Scotty, implemented within the Phasertng codebase, was used to perform a PDB‐wide analysis of lattice coincidences.
Airlie J. McCoy +3 more
wiley +1 more source
Zinc oxide (ZnO) and bacteriorhodopsin (bR) hybrid nanostructures were fabricated by immobilizing bR on ZnO thin films and ZnO nanorods. The morphological and spectroscopic analysis of the hybrid structures confirmed the ZnO thin film/nanorod growth and ...
Saurav Kumar +7 more
doaj +1 more source
Es werde Licht! Licht als Antrieb und Regulator in synthetischen Zellen
Zellen sind Systeme außerhalb des Gleichgewichts, die ihre Eigenschaften in Raum und Zeit regulieren. In biomimetischen synthetischen Zellen bietet Licht eine einzigartige Möglichkeit, Vorgänge räumlich und zeitlich anzutreiben und zu kontrollieren.
Matthew E. Allen +5 more
wiley +1 more source
Let There be Light! Light as an Engine and Regulator in Synthetic Cells
Cells are out‐of‐equilibrium systems that regulate their spatiotemporal features. In biomimetic synthetic cells, light offers a unique way for energizing and regulating events in space and time. This review explores the implementation of light‐responsive tools in synthetic cells to replicate biological functions and enable new bioinspired behaviors ...
Matthew E. Allen +5 more
wiley +1 more source
Role of internal water molecules in bacteriorhodopsin [PDF]
Internal water molecules are considered to play a crucial role in the functional processes of proton pump proteins. They may participate in hydrogen-bonding networks inside proteins that constitute proton pathways.
Hideki Kandori, Kandori, Hideki
core +1 more source

