Engineering of High‐Yield Recombinant Adeno‐Associated Virus Producer Plasmids
Biotechnology Journal, Volume 21, Issue 1, January 2026.Graphical Abstract and Lay Summary Recombinant adeno‐associated viruses (AAV) are important in gene therapy but their production may be impaired by non‐optimized gene design. In this study, the authors demonstrate that production was best when the AAV replicase gene was in a wild‐type configuration while the complexity of other genetic elements could ...
Marco T. Radukic +10 more
wiley +1 more source
Ultra Deep Sequencing of a Baculovirus Population Reveals Widespread Genomic Variations
Viruses, 2015 Viruses rely on widespread genetic variation and large population size for adaptation. Large DNA virus populations are thought to harbor little variation though natural populations may be polymorphic.
A. Chateigner +5 more
semanticscholar +1 more source
Engineering a serum-resistant and thermostable vesicular stomatitis virus G glycoprotein for pseudotyping retroviral and lentiviral vectors. [PDF]
, 2013 Vesicular stomatitis virus G glycoprotein (VSV-G) is the most widely used envelope protein for retroviral and lentiviral vector pseudotyping; however, serum inactivation of VSV-G pseudotyped vectors is a significant challenge for in vivo gene delivery ...
Hwang, B-Y, Schaffer, DV
core
Profiling time course expression of virus genes---an illustration of Bayesian inference under shape restrictions [PDF]
, 2010 There have been several studies of the genome-wide temporal transcriptional program of viruses, based on microarray experiments, which are generally useful in the construction of gene regulation network.
Chang, I-Shou +6 more
core +2 more sources
Pyroptosis in Peripheral Neuropathy: From Molecular Mechanisms to Therapeutic Targeting
CNS Neuroscience &Therapeutics, Volume 32, Issue 1, January 2026.Pyroptosis is a key inflammatory cell death pathway in peripheral neuropathy, with opposing roles: it perpetuates neuropathic pain but can be induced to kill neuroblastoma cells. This review consolidates mechanistic understanding and therapeutic strategies, arguing for a shift from disease‐centric to target‐centric treatment approaches to improve ...
Jinhuan Wei +11 more
wiley +1 more source
Recombinant monovalent llama-derived antibody fragments (VHH) to rotavirus VP6 protect neonatal gnotobiotic piglets against human rotavirus-induced diarrhea [PDF]
, 2013 Group A Rotavirus (RVA) is the leading cause of severe diarrhea in children. The aims of the present study were to determine the neutralizing activity of VP6-specific llama-derived single domain nanoantibodies (VHH nanoAbs) against different RVA strains ...
Alvarado, Carmen +14 more
core +3 more sources
Modulators of Diacylglycerol Kinase Activity: A Review of Advances and Challenges
Medicinal Research Reviews, Volume 46, Issue 1, Page 149-175, January 2026.ABSTRACT Catalyzing the conversion of diacylglycerol (DAG) in phosphatidic acid (PA), diacylglycerol kinases (DGKs) play a pivotal role in all the physiological processes modulated by these two bioactive lipids, such as lipid metabolism and immune regulation.
Luisa Racca +2 more
wiley +1 more source
Directional and direct cloning strategy for high-throughput generation of recombinant baculoviruses
BioTechniques, 2006 The baculovirus expression vector system (BEVS) has become one of the most widely used systems for routine protein expression. We have developed an improved strategy to clone foreign genes directionally and directly into the baculovirus genome vector via
Qi Ma, Li Zhou, Lixin Ma, Keke Huo
doaj +1 more source
BMC Biotechnology, 2018 Background The Baculovirus expression vector system (BEVS) is a transient expression platform for recombinant protein production in insect cells. Baculovirus infection of insect cells will shutoff host translation and induce apoptosis and lead to the ...
Xiaoyue Zhang +4 more
doaj +1 more source