Results 141 to 150 of about 39,940 (191)
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Baculovirus structural polypeptides
Virology, 1978Abstract The structural polypeptides of eight insect baculoviruses were studied using vertical slab polyacrylamide gel electrophoresis. All viruses revealed a complex but unique composition of 15 to 25 bands with molecular weights ranging from 15,000 to 160,000. Since certain baculoviruses have more than one nucleocapsid per viral envelope (multiples)
M D, Summers, G E, Smith
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Transfection with baculovirus DNA
Virology, 1980Purified DNA from the nuclear polyhedrosis viruses of Autographa californica (AcM NPV) and Rachiplusia ou (RoMNPV) were found to be infectious in TN-368 cells employing the calcium phosphate precipitation technique (F. L. Graham and A. J. van der Eb, Virology, 52 ,456-467, 1973).
J P, Burand, M D, Summers, G E, Smith
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Bioprocessing of Baculovirus Vectors: A Review
Current Gene Therapy, 2010The baculovirus/insect cell expression system is best known, and used, as a research tool for the production of recombinant proteins often requiring post-translational modifications. Although studies on the larger scale use of the system have been reported on for three decades, the recognition of this system as an industrially relevant platform for the
Aucoin, Marc G. +2 more
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Manipulation of Baculovirus Vectors
2003The development of expression vectors from baculoviruses has provided molecular biologists with one of the most efficient and effective ways of synthesizing foreign gene products in a heterologous system. In contrast to the other recent excellent reviews on the subject (1,2), this contribution aims to explore the various practical aspects of the system,
M J, Bailey, R D, Possee
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Replication of the Baculovirus Genome
Molecular Biology, 2003The review describes the current state of studying the baculovirus DNA replication. The structural organization of replication initiation sites and replication intermediates are considered. Attention is focused on virus replication factors, including DNA polymerase, helicase, IE-1, LEF-1, LEF-2, and LEF-3.
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Introduction to Baculovirus Molecular Biology
2007The development of baculovirus expression vector systems has accompanied a rapid expansion of our knowledge about the genes, their function, and regulation in insect cells. Classification of these viruses has also been refined as we learn more about differences in gene content between isolates, how this affects virus structure, and their replication in
Kelly, Barbara J. +2 more
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Construction of baculovirus recombinants
Cytotechnology, 1996There are three main reasons for wishing to modify baculovirus genomes. The most common arises from the use of baculoviruses to express foreign genes. Baculovirus expression systems frequently give high level expression of proteins with full biological activity.
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Application of Baculovirus Expression Vector System (BEVS) in Vaccine Development
Vaccines, 2023Yang Wang, Wei Xiawei, Wei Yuquan
exaly

