Results 131 to 140 of about 68,162 (268)

Evaluation of recombinant baculovirus clearance during rAAV production in Sf9 cells using a newly developed fluorescent-TCID50 assay

Frontiers in Medicine
IntroductionRecombinant adeno-associated virus (rAAV) vectors provide a safe and efficient means for in vivo gene delivery, although its large-scale production remains challenging. Featuring high manufacturing speed, flexible product design, and inherent
Ningguang Luo, Xiangqun Chen, Jinzhong Li, Derrick Huynh, Ying Li, Li Ou, Shengjiang Liu   +6 more
doaj   +1 more source

Fusion to green fluorescent protein improves expression levels of Theileria parva sporozoite surface antigen p67 in insect cells

, 2002
East Coast fever (ECF) is a fatal disease of cattle caused by the protozoan parasite Theileria parva. The development of a subunit vaccine, based on the sporozoite-specific surface antigen p67, has been hampered by difficulties in achieving high-level ...
Kaba, S.A., Nene, V., Musoke, A.J., Vlak, J.M., Oers, M.M., van   +4 more
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Protein expression in the baculovirus-insect cell expression system

, 2019
The baculovirus-insect cell expression system is a popular choice for recombinant protein production. Post-translational modifications, production of protein complexes, and reported high protein yields are some of the favorable features of this ...
Adetayo A. Adeniyi, Adeniyi, Adetayo A., Lua, Linda Hwee-Lin, Linda Hwee-Lin Lua   +3 more
core   +1 more source

Method of producing baculovirus

, 2005
The present invention is directed to a method for producing commercial quantities of Baculovirus using a combination of methods involving producing occlusion bodies with infectious baculovirus in caterpillar larvae and large numbers of viral particles ...
Lua, Linda, Reid, Steven
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Baculovirus dual promoter expression vector

, 1990
This invention relates to specifically designing and genetically engineering recombinant baculovirus for producing, in a compatible insect system, a desired protein, virus, protein hybrid, or virus hybrid. In particular aspects, this invention relates to
Oker-blom, Christian E. G., Summers, Max D.   +1 more
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Genomic support for speciation and specificity of baculoviruses

, 2010
Keywords: baculovirus, insects, speciation, genomics, phylogeny, host specificity The Baculoviridae comprise a large family of double-stranded DNA viruses infecting arthropods.
Jakubowska, A.K.
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Baculovirus expression systems

, 2010
Baculoviruses are insect-specific pathogens, producing occlusion bodies containing enveloped virus particles which spread infection between insects. These occlusions or polyhedra are composed mainly of a 30 KDa virus-encoded protein produced in the very ...
Hitchman, Richard B, Robert D. Possee, Richard B. Hitchman, King, Linda A, Possee, Robert D, Linda A. King   +5 more
core   +1 more source

Improved baculovirus expression systems

, 2013
The present invention relates to an optimized baculovirus construct useful for the production of virus ( like) particles or viral vectors in particular viral vectors for gene ...
Galibert, L., Riviere, C., van Oers, M.M., Oers, M.M., van, Merten, O.W.   +4 more
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LEF-6 phosphorylation regulates the binding and trafficking of baculovirus late gene transcripts

Journal of Virology
The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) LEF-6 has been shown to accelerate the infection cycle of AcMNPV, but its specific function is yet to be defined.
Xiaoyue Zhang, Zhihui Zhang, Ziqian Cao, Jianchao Li, Shengqiang Jiang, Hongying Chen   +5 more
doaj   +1 more source

Sequencing of Recombinant Baculovirus Vectors - p6.9 rBEV

2nd passage (P2) recombinant baculovirus vectors having a reporter green fluorescent protein (mAzami Green) under the control of the p6.9 promoter. The recombinant viruses were amplified in Spodoptera frugiperda (Sf9) cells at a low multiplicity of ...
Nielsen, Lisa, Nash, Delaney, Nissimov, Jozef I., Chakraborty, Madhuja, Charles, Trevor, Aucoin, Marc G.   +5 more
core   +1 more source