Type I-E CRISPR-cas systems discriminate target from non-target DNA through base pairing-independent PAM recognition. [PDF]
PLoS Genetics, 2013 Discriminating self and non-self is a universal requirement of immune systems. Adaptive immune systems in prokaryotes are centered around repetitive loci called CRISPRs (clustered regularly interspaced short palindromic repeat), into which invader DNA ...
Edze R Westra +6 more
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Unusual Base-Pairing Interactions in Monomer–Template Complexes
ACS Central Science, 2016 Many high-resolution crystal structures have contributed to our understanding of the reaction pathway for catalysis by DNA and RNA polymerases, but the structural basis of nonenzymatic template-directed RNA replication has not been studied in comparable ...
Wen Zhang +3 more
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Spontaneous formation and base pairing of plausible prebiotic nucleotides in water
Nature Communications, 2016 One of the questions for prebiotic chemistry is the formation of complementary base pairing systems. Here, the authors show that plausible two prebiotic heterocycles can form glycosidic bonds with ribose in water and that these spontaneously base-pair in
Brian J. Cafferty +4 more
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Hydrogen Bonding (Base Pairing) in Antiviral Activity. [PDF]
Viruses, 2023 Base pairing based on hydrogen bonding has, since its inception, been crucial in the antiviral activity of arabinosyladenine, 2′-deoxyuridines (i.e., IDU, TFT, BVDU), acyclic nucleoside analogues (i.e., acyclovir) and nucleoside reverse transcriptase ...
De Clercq E.
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Watson-Crick Base Pairing of N-Methoxy-1,3-Oxazinane (MOANA) Nucleoside Analogues within Double-Helical DNA. [PDF]
ChemistryOpen, 2023 Hairpin oligodeoxynucleotides incorporating a (2R,3S)‐4‐(methoxyamino)butane‐1,2,3‐triol residue in the middle of the double‐helical stem and opposite to either one of the canonical nucleobases or an abasic 2‐(hydroxymethyl)tetrahydrofuran‐3‐ol spacer ...
Afari MNK +3 more
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Constraint of Base Pairing on HDV Genome Evolution. [PDF]
Viruses, 2021 The hepatitis delta virus is a single-stranded circular RNA virus, which is characterized by high self-complementarity. About 70% of the genome sequences can form base-pairs with internal nucleotides.
Nagata S, Kiyohara R, Toh H.
europepmc +2 more sources
Sequence dependence of transient Hoogsteen base pairing in DNA. [PDF]
PLoS Comput Biol, 2022 Hoogsteen (HG) base pairing is characterized by a 180° rotation of the purine base with respect to the Watson-Crick-Franklin (WCF) motif. Recently, it has been found that both conformations coexist in a dynamical equilibrium and that several biological ...
Pérez de Alba Ortíz A +2 more
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Watson-Crick Base-Pairing Requirements for ssDNA Recognition and Processing in Replication-Initiating HUH Endonucleases. [PDF]
mBio, 2023 Replication-initiating HUH endonucleases (Reps) are sequence-specific nucleases that cleave and rejoin single-stranded DNA (ssDNA) during rolling-circle replication.
Smiley AT +7 more
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Synthesis of 6-Alkynylated Purine-Containing DNA via On-Column Sonogashira Coupling and Investigation of Their Base-Pairing Properties. [PDF]
Molecules, 2023 Synthetic unnatural base pairs have been proven to be attractive tools for the development of DNA-based biotechnology. Our group has very recently reported on alkynylated purine–pyridazine pairs, which exhibit selective and stable base-pairing via ...
Okamura H +4 more
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An adenosine analog shows high antiviral potency against coronavirus and arenavirus mainly through an unusual base pairing mode. [PDF]
Nat CommunBy targeting the essential viral RNA-dependent RNA polymerase (RdRP), nucleoside analogs (NAs) have exhibited great potential in antiviral therapy for RNA virus-related diseases.
Jia X +19 more
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