Results 51 to 60 of about 353,589 (332)
A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins
FEBS Letters, EarlyView.We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue +6 more
wiley +1 more source
Results in Chemistry, 2020 The tandem repeat stretches of cytosine residues fold to construct intercalated motif (i-motif) structures over an acidic pH range. Several advancements have been made to understand the biological role of i-motif DNA.
Swati Chaudhary +3 more
doaj +1 more source
A Statistical Analysis of RNA Folding Algorithms Through Thermodynamic Parameter Perturbation [PDF]
, 2004 Computational RNA secondary structure prediction is rather well established. However, such prediction algorithms always depend on a large number of experimentally measured parameters.
Bundschuh, R., Layton, D. M.
core +3 more sources
FEBS Letters, EarlyView.Bifidobacterium bifidum establishes symbiosis with infants by metabolizing lacto‐N‐biose I (LNB) from human milk oligosaccharides (HMOs). The extracellular multidomain enzyme LnbB drives this process, releasing LNB via its catalytic glycoside hydrolase family 20 (GH20) lacto‐N‐biosidase domain.
Xinzhe Zhang +5 more
wiley +1 more source
Comment on “Flexibility of short DNA helices with finite-length effect: From base pairs to tens of base pairs” [J. Chem. Phys. 142, 125103 (2015)] [PDF]
, 2021 Midas Segers +5 more
openalex +1 more source
FEBS Letters, EarlyView.This perspective highlights emerging insights into how the circadian transcription factor CLOCK:BMAL1 regulates chromatin architecture, cooperates with other transcription factors, and coordinates enhancer dynamics. We propose an updated framework for how circadian transcription factors operate within dynamic and multifactorial chromatin landscapes ...
Xinyu Y. Nie, Jerome S. Menet
wiley +1 more source
Selecting against accidental RNA interactions
eLife, 2016 Random base-pairing interactions between messenger RNAs and noncoding RNAs can reduce translation efficiency.
Meredith Corley, Alain Laederach
doaj +1 more source
Cis- and Trans-Encoded Small Regulatory RNAs in Bacillus subtilis
Microorganisms, 2021 Small regulatory RNAs (sRNAs) that act by base-pairing are the most abundant posttranscriptional regulators in all three kingdoms of life. Over the past 20 years, a variety of approaches have been employed to discover chromosome-encoded sRNAs in a ...
Sabine Brantl, Peter Müller
doaj +1 more source
Base Pairs to Populations [PDF]
Clinical Cancer Research, 2017 “There is much work to be done and many challenges exist in our mission to end the suffering that breast cancer brings.” So conclude Guest Editors Ann Partridge and Lisa Carey in their overview for this CCR Focus on breast cancer.
openaire +2 more sources
Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer
FEBS Letters, EarlyView.Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani +4 more
wiley +1 more source