Results 91 to 100 of about 965,609 (309)

Diagnostic value of 3D-Cube T2 sequence combined with plain CT scan of the skull base for cerebrospinal fluid rhinorrhea

Di-san junyi daxue xuebao, 2019
Objective To evaluate the diagnostic value of magnetic resonance 3D-Cube T2 sequence, plain CT scan of the skull base and their combination for cerebrospinal fluid (CSF) rhinorrhea and assess the performance of these 2 modalities combined in improving ...
GAN Hui, ZHANG Song, JIANG Liling, GE Xiaodong, ZOU Liguang   +4 more
doaj   +1 more source

Improving the specificity of nucleic acid detection with endonuclease-actuated degradation

Communications Biology, 2022
A new method, SENTINEL, improves the specificity of nucleic acid target sequence identification via a combination of isothermal amplification and sequence-specific DNA endonucleases. The method can discriminate single base-pair differences and takes less
Roger S. Zou, Momcilo Gavrilov, Yang Liu, Dominique Rasoloson, Madison Conte, Justin Hardick, Leo Shen, Siqi Chen, Andrew Pekosz, Geraldine Seydoux, Yukari C. Manabe, Taekjip Ha   +11 more
doaj   +1 more source

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Contribution of nonconsensus base pairs within ArsR binding sequences toward ArsR-DNA binding and arsenic-mediated transcriptional induction

Journal of Biological Engineering, 2019
Background A transcriptional reporter is the key component in bacterial biosensors which are employed to monitor the induction or repression of a reporter gene corresponding to environmental change.
Xingjuan Chen, Xin Jiang, Cuijuan Tie, Jinnon Yoo, Yan Wang, Meiying Xu, Guoping Sun, Jun Guo, Xianqiang Li   +8 more
doaj   +1 more source

Fundamental sequences based on localization

Annals of Pure and Applied Logic
Extended version of the paper published under the same title in the LNCS proceedings of the 20th Conference on Computability in Europe (CiE) 2024, containing complete proofs and generalization to simultaneously defined collapsing functions First revision: Overall improvement of readability.
openaire   +2 more sources

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Application of massive parallel sequencing to whole genome SNP discovery in the porcine genome [PDF]

, 2009
Background Although the Illumina 1 G Genome Analyzer generates billions of base pairs of sequence data, challenges arise in sequence selection due to the varying sequence quality.
Kerstens Hindrik HD, Heuven, H.C.M., Johan T den Dunnen, Henri CM Heuven, Andreia J Amaral, den Dunnen Johan T, Dibbits Bert, Groenen Martien AM, Heuven Henri CM, Megens, H.J.W.C., Amaral Andreia J, Megens Hendrik-Jan, Bert Dibbits, Martien AM Groenen, Groenen, M.A.M., Hendrik-Jan Megens, Dibbits, B.W., Dungen, J., den, Kerstens, H.H.D., Hindrik HD Kerstens, Richard PMA Crooijmans, Crooijmans, R.P.M.A., den Dungen, J., Crooijmans Richard PMA, Amaral, A.J.   +24 more
core   +1 more source

Decoupling of Bypass Efficiency and Mutagenicity of the 2-Acetylaminofluorene C8-Guanine Adduct by DNA Sequence Context

Toxics
DNA sequence context plays a critical role in modulating the mutational effects of DNA damage. Here, we investigated how base identity influences the replication bypass and mutagenicity of a site-specific dG-AAF (2-acetylaminofluorene) bulky adduct in ...
Yi-Tzai Chen, Rui Qi, Jian Ma, Ang Cai, Bongsup P. Cho, Deyu Li   +5 more
doaj   +1 more source

The complete mitogenome and phylogenetic analysis of Japanese firefly ‘Genji Botaru’ Luciola cruciata (Coleoptera: Lampyridae)

Mitochondrial DNA. Part B. Resources, 2017
We performed mitogenome analysis of Japanese firefly Luciola cruciata (Coleoptera: Lampyridae), which is unique species in Japan. It is classified into six haplotypes based on the difference of COII sequence on mitochondrial DNA.
Juri Maeda, Dai-ichiro Kato, Kazunari Arima, Yuji Ito, Atsushi Toyoda, Hideki Noguchi   +5 more
doaj   +1 more source