Results 271 to 280 of about 2,954,117 (341)

CarpeDeam: a de novo metagenome assembler for heavily damaged ancient datasets. [PDF]

Genome Biol
Kraft L, Söding J, Steinegger M, Jochheim A, Wad Sackett P, Fernandez-Guerra A, Renaud G.   +6 more
europepmc   +1 more source

Overexpression of CDT1 inhibits cell cycle progression at S phase by interacting with the mini‐chromosome maintenance complex and causes DNA damage

FEBS Open Bio, EarlyView.
CDT1 is an essential protein for DNA replication licensing that loads the MCM complex, the eukaryotic replicative DNA helicase, onto replication origins. Overexpression of CDT1 induces cell cycle arrest at the S phase. Here we showed CDT1 inhibits the progression of replication forks by interacting with the MCM complex, leading to the stalling and ...
Takashi Tsuyama, Nonoka Takayama, Rina Tanaka, Yuuki Arai, Yohko Yamaguchi, Yuko Nawata, Yutaro Azuma, Shusuke Tada   +7 more
wiley   +1 more source

A general model for analysis of linear and hyperbolic enzyme inhibition mechanisms

FEBS Open Bio, EarlyView.
We developed a general enzyme kinetic model that integrates these six basic inhibition mechanism onto a single one. From this model, we deduced a general enzyme kinetic equation that through modulation of simple parameters, γ (the relative inhibitor affinity for two binding sites) and β (the reactivity of the enzyme–substrate–inhibitor complex), is ...
Rafael S. Chagas, Sandro R. Marana
wiley   +1 more source

Effective in vivo RNA base editing via engineered cytidine deaminase APOBECs fused with PUF proteins. [PDF]

Nat Commun
Han W, Yuan B, Fan X, Li W, Yuan Y, Zhang Y, Wang S, Shan S, Hafner M, Wang Z, Qiu Z.   +10 more
europepmc   +1 more source

HSP70 governs permeability and mechanotransduction in primary human endothelial cells

FEBS Open Bio, EarlyView.
HSP70 chemical inhibition reduces endothelial cell proliferation and increases permeability, the latter supported by normal interendothelial junctional protein distribution. HSP70 also plays a role in shear stress response, a hemodynamic force naturally present in blood vessels and correlated with vessel protection.
Andrea Pinto‐Martinez, Everton G. Melo, Isadora C. B. Pavan, Percíllia V. S. Oliveira, Luiza B. C. T. Coimbra, Thaís L. S. Araujo   +5 more
wiley   +1 more source

Synthesis and Incorporation of a pH-Responsive Nucleoside Into DNA Sequences. [PDF]

Chembiochem
Ogel E, Becker S.
europepmc   +1 more source

Phylogenetic Study of Several Parasitic Plant Species Based on The atp-1 Gene Sequence

Pratama Ardo Cahya, Nadaa Karuniasari, Agnia Fadillah Rahmadini, A. G. Ezra, Victor Alvianoes Guterez Hose, Turhadi Turhadi, Eko Suyanto, Fatchiyah Fatchiyah   +7 more
openalex   +1 more source

Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein

FEBS Open Bio, EarlyView.
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley   +1 more source

Training Recurrent Neural Networks for BrdU Detection with Oxford Nanopore Sequencing: Guidance and Lessons Learned. [PDF]

Genes (Basel)
Liu H, Flavahan W, Zhu LJ.
europepmc   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source