Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley +1 more source
Dynamic activation of basilar membrane macrophages in response to chronic sensory cell degeneration in aging mouse cochleae. [PDF]
Frye MD+4 more
europepmc +1 more source
Fabrication and Vibration Characterization of a Partially Etched-type Artificial Basilar Membrane
Hanmi Kang+5 more
openalex +2 more sources
Matrix: a complex amalgam of structures and functions in tumor microenvironment
The matrix is a dynamic, intricate three‐dimensional mesh of biomolecules with both structural and functional properties. This review deals with the complexity of this ‘molecular amalgam’ in the tumor microenvironment and highlights its importance in the maintenance and evolution of tumors by describing certain matrix biomolecules, such as ...
Spyros S. Skandalis+3 more
wiley +1 more source
Preparation and characterization of renal cell peptides from fetal rats for their antitumor activity
This study aimed to prepare renal cells (RCs) from fetal rats which were digested by enzymes. Candidate peptides RCPs were characterized by capillary HPLC and MS and their bioactivity was predicted using peptideranker. The predicted top 10 bioactive peptides were synthesized.
Zhe Zhang+6 more
wiley +1 more source
Basilar membrane and tectorial membrane stiffness in the CBA/CaJ mouse. [PDF]
Teudt IU, Richter CP.
europepmc +1 more source
The effect of endolymphatic potential on basilar membrane linearity [PDF]
David C. Mountain
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Phenotypic plasticity in a newly established set of EGFR inhibitor‐adapted NSCLC cell lines during adaptation and in established cell lines. Here, we introduce novel sublines of the EGFR‐mutant non‐small cell lung cancer (NSCLC) cell lines HCC827 and HCC4006 adapted to the EGFR kinase inhibitors gefitinib (HCC827rGEFI2μm, HCC4006rGEFI1μm), erlotinib ...
Tharsagini V. Nanthaprakash+6 more
wiley +1 more source
The plateaus in basilar membrane vibration data—explained [PDF]
Max A. Viergever
openalex +1 more source
Ears adapted for the detection of motion, or how echolocating bats have exploited the capacities of the mammalian auditory system [PDF]
Bruns, V.+2 more
core +1 more source