Results 51 to 60 of about 829,070 (291)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source

Broadband terahertz surface plasmon-polaritons beam splitter

, 2021
In this paper, we propose a novel design of broadband terahertz (THz) surface plasmon-polaritons (SPPs) beam splitter, which obtains 50%-50% energy splitting on input and output SPPs waveguides.
Mai Liu, Wei Huang, Yu Cheng, Shan Yin, Weifang Yang, Wentao Zhang   +5 more
core   +1 more source

Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells

FEBS Open Bio, EarlyView.
Microscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Mark A. Hink
wiley   +1 more source

Characterization of Defect Distribution in an Additively Manufactured AlSi10Mg as a Function of Processing Parameters and Correlations with Extreme Value Statistics

Advanced Engineering Materials, EarlyView.
Predicting extreme defects in additive manufacturing remains a key challenge limiting its structural reliability. This study proposes a statistical framework that integrates Extreme Value Theory with advanced process indicators to explore defect–process relationships and improve the estimation of critical defect sizes. The approach provides a basis for
Muhammad Muteeb Butt, Varma Kvvssn, Hossein Laieghi, Zia Uddin, Peyman Ansari, E. Kıvanç Sadak, Can Barış Toprak, Hüseyin Kızıl, Enrico Salvati   +8 more
wiley   +1 more source

Flexural Behavior of 3D‐Printed Porous Bouligand‐Structured Polymers: Influence of Pitch Angle and Porosity

Advanced Engineering Materials, EarlyView.
This study examines how helicoidal architectures with different porosities respond to bending. Adjusting layer angle and spacing in 3D‐printed polymers reveals clear tradeoffs between stiffness, strength, and energy absorption. Experiments and simulations highlight designs that distribute stress effectively, offering pathways for optimizing lightweight
Praveenkumar Subhash Patil, Edward D. McCarthy, Parvez Alam   +2 more
wiley   +1 more source

Design of Photonic Molecule-Based Multiway Beam Splitter/Coupler with Variable Division Ratio

Photonics
An optical beam splitter is used for dividing an input optical beam into several separate beams with a specific power ratio. Usually, conventional optical beam splitters have bulky dimensions (many optical wavelengths) and a fixed dividing ratio, which ...
Yury E. Geints
doaj   +1 more source

Invited Article: Quantum memristors in quantum photonics

APL Photonics, 2018
We propose a method to build quantum memristors in quantum photonic platforms. We first design an effective beam splitter, which is tunable in real time, by means of a Mach-Zehnder-type array with two equal 50:50 beam splitters and a tunable retarder ...
M. Sanz, L. Lamata, E. Solano
doaj   +1 more source

Numerical analysis of temperature-controlled terahertz power splitter

Journal of the European Optical Society-Rapid Publications, 2017
Background As a significant terahertz functional device, terahertz beam splitters with high performance are highly required to meet the need for terahertz communication, terahertz image, and terahertz sensor systems.
Li Yang, Li Jiu-Sheng
doaj   +1 more source

A Novel Method for Quadrature Signal Construction in a Semiconductor Self-Mixing Interferometry System Using a Liquid Crystal Phase Shifter

Photonics, 2023
We have established a novel method for quadrature signal construction in a semiconductor laser diode self-mixing interferometry system using two photodiodes and a beam splitter with a liquid crystal phase shifter (LCPS).
Yancheng Li, Zenghui Peng, Xiao Shen, Junfeng Wu   +3 more
doaj   +1 more source