Results 1 to 10 of about 62,937 (332)

On the evolution of beta-galactosidase. [PDF]

Proceedings of the National Academy of Sciences, 1978
The amino acid sequence of beta-galactosidase (beta-D-galactoside galactohydrolase, EC 3.2.1.23) has been compared to itself and to other proteins. Two segments, each of about 380 amino acids, comprising the first three-fourths of the polypeptide chain, were found to be very similar to each other. It is concluded that they are homologous.
J.M. Hood, A.V. Fowler, Irving Zabin
openalex   +4 more sources

Genetic Fusion of an Anti-BclA Single-Domain Antibody with Beta Galactosidase [PDF]

Antibodies, 2018
The Bacillus collagen-like protein of anthracis (BclA), found in Bacillus anthracis spores, is an attractive target for immunoassays. Previously, using phage display we had selected llama-derived single-domain antibodies that bound to B.
George P. Anderson, Lisa C. Shriver-Lake, Scott A. Walper, Lauryn Ashford, Dan Zabetakis, Jinny L. Liu, Joyce C. Breger, P. Audrey Brozozog Lee, Ellen R. Goldman   +8 more
doaj   +2 more sources

Senescence‐associated β‐galactosidase is lysosomal β‐galactosidase [PDF]

Aging Cell, 2006
SummaryReplicative senescence limits the proliferation of somatic cells passaged in culture and may reflect cellular aging in vivo. The most widely used biomarker for senescent and aging cells is senescence‐associated β‐galactosidase (SA‐β‐gal), which is defined as β‐galactosidase activity detectable at pH 6.0 in senescent cells, but the origin of SA‐β‐
Bo Yun Lee, Wim J. Kleijer, Daniel DiMaio, Jun Sub Im, Amelia Morrone, Jung A. Han, Eun Seong Hwang, Eun Seong Hwang, Kimberly L. Johung, Edward C. Goodwin   +9 more
openaire   +8 more sources

General Properties of Beta-Galactosidase of Xanthomonas campestris [PDF]

Applied and Environmental Microbiology, 1979
Partially purified β-galactosidase of Xanthomonas campestris required 32 to 37 C and pH 5.5 to 5.8 for optimum activity. The enzyme had low affinity for lactose hydrolysis ( K m = 22 mM) and was inhibited by thiol group reagents ...
Joseph F. Frank, George A. Somkuti
openalex   +4 more sources

Molecular basis of beta-galactosidase alpha-complementation. [PDF]

Proceedings of the National Academy of Sciences, 1975
In previous studies, a cyanogen bromide peptide derived from amino-acid residues 3-92 of beta-galactosidase (EC 3.2.1.23; beta-D-galactoside galactohydrolase) was shown to have alpha-donor activity in intracistronic alpha-complementation. We have now isolated the defective beta-galactosidase alpha-acceptor protein from the deletion mutant strain M15 of
K E Langley, Merna Villarejo, A.V. Fowler, Patrice J. Zamenhof, Irving Zabin   +4 more
openalex   +4 more sources

Safety evaluation of the food enzyme beta‐galactosidase from Bacillus sp. (strain M3‐1) [PDF]

EFSA Journal, 2019
The food enzyme β‐d‐galactosidase galactohydrolase (EC 3.2.1.23) is produced with Bacillus sp. strain M3‐1 by GenoFocus Inc. The food enzyme β‐galactosidase is intended to be used in the manufacture of galactooligosaccharides (GOS).
EFSA Panel on Food Contact Materials, Enzymes and Processing Aids (CEP), Vittorio Silano, José Manuel Barat Baviera, Claudia Bolognesi, Pier Sandro Cocconcelli, Riccardo Crebelli, David Michael Gott, Konrad Grob, Evgenia Lampi, Alicja Mortensen, Gilles Rivière, Inger‐Lise Steffensen, Christina Tlustos, Henk Van Loveren, Laurence Vernis, Holger Zorn, Klaus‐Dieter Jany, Francesca Marcon, André Penninks, Jaime Aguilera, Magdalena Andryszkiewicz, Natália Kovalkovičová, Yi Liu, Andrew Chesson   +23 more
doaj   +2 more sources

Thermostable Beta-Galactosidase from Fungi

Journal of Dairy Science, 1982
Abstract Forty-five strains of molds were screened for beta-galactosidase activity. Strains exhibiting enzyme activity were evaluated for cell yield as well as enzyme units available per liter of the medium, per gram cell dry weight, and per milligram protein in their cell free extracts.
Sanjeev Agrawal, H. M. Sonawat, Santanu Dutta   +2 more
openalex   +3 more sources

beta-Galactosidase chimeras: primary structure of a lac repressor-beta-galactosidase protein. [PDF]

Proceedings of the National Academy of Sciences, 1978
A protein possessing both lac repressor and beta-galactosidase activities in a single polypeptide of about 155,000 daltons was purified from a deletion mutant of Escherichia coli in which the lacI and Z genes are fused. A 77-residue cyanogen bromide peptide containing the fusion joint was isolated.
Jürgen Kania, Irving Zabin, Anthony J. Brake, Benno Müller-Hill, Audree V. Fowler   +4 more
openaire   +3 more sources

Rapid Immunochromatographic Detection of Serum Anti-α-Galactosidase A Antibodies in Fabry Patients after Enzyme Replacement Therapy. [PDF]

PLoS ONE, 2015
We developed an immunochromatography-based assay for detecting antibodies against recombinant α-galactosidase A proteins in serum. The evaluation of 29 serum samples from Fabry patients, who had received enzyme replacement therapy with agalsidase alpha ...
Sachie Nakano, Takahiro Tsukimura, Tadayasu Togawa, Toya Ohashi, Masahisa Kobayashi, Katsuyoshi Takayama, Yukuharu Kobayashi, Hiroshi Abiko, Masatsugu Satou, Tohru Nakahata, David G Warnock, Hitoshi Sakuraba, Futoshi Shibasaki   +12 more
doaj   +1 more source

Cell permeabilization of Kluyveromyces and Saccharomyces species to obtain potential biocatalysts for lactose hydrolysis

Acta Scientiarum: Biological Sciences, 2022
Yeast’s beta-galactosidase is an intracellular enzyme, through which it is possible to determine in vivo its activity as a biocatalyst in the lactose hydrolysis.
Érika de Pádua Alves, Alessandra Bosso, Luiz Rodrigo Ito Morioka, Hélio Hiroshi Suguimoto   +3 more
doaj   +1 more source