Results 1 to 10 of about 56,336 (178)

Genetic Fusion of an Anti-BclA Single-Domain Antibody with Beta Galactosidase [PDF]

Antibodies, 2018
The Bacillus collagen-like protein of anthracis (BclA), found in Bacillus anthracis spores, is an attractive target for immunoassays. Previously, using phage display we had selected llama-derived single-domain antibodies that bound to B.
George P. Anderson, Lisa C. Shriver-Lake, Scott A. Walper, Lauryn Ashford, Dan Zabetakis, Jinny L. Liu, Joyce C. Breger, P. Audrey Brozozog Lee, Ellen R. Goldman   +8 more
doaj   +2 more sources

Safety evaluation of the food enzyme beta‐galactosidase from Bacillus sp. (strain M3‐1) [PDF]

EFSA Journal, 2019
The food enzyme β‐d‐galactosidase galactohydrolase (EC 3.2.1.23) is produced with Bacillus sp. strain M3‐1 by GenoFocus Inc. The food enzyme β‐galactosidase is intended to be used in the manufacture of galactooligosaccharides (GOS).
EFSA Panel on Food Contact Materials, Enzymes and Processing Aids (CEP), Vittorio Silano, José Manuel Barat Baviera, Claudia Bolognesi, Pier Sandro Cocconcelli, Riccardo Crebelli, David Michael Gott, Konrad Grob, Evgenia Lampi, Alicja Mortensen, Gilles Rivière, Inger‐Lise Steffensen, Christina Tlustos, Henk Van Loveren, Laurence Vernis, Holger Zorn, Klaus‐Dieter Jany, Francesca Marcon, André Penninks, Jaime Aguilera, Magdalena Andryszkiewicz, Natália Kovalkovičová, Yi Liu, Andrew Chesson   +23 more
doaj   +2 more sources

Rapid Immunochromatographic Detection of Serum Anti-α-Galactosidase A Antibodies in Fabry Patients after Enzyme Replacement Therapy. [PDF]

PLoS ONE, 2015
We developed an immunochromatography-based assay for detecting antibodies against recombinant α-galactosidase A proteins in serum. The evaluation of 29 serum samples from Fabry patients, who had received enzyme replacement therapy with agalsidase alpha ...
Sachie Nakano, Takahiro Tsukimura, Tadayasu Togawa, Toya Ohashi, Masahisa Kobayashi, Katsuyoshi Takayama, Yukuharu Kobayashi, Hiroshi Abiko, Masatsugu Satou, Tohru Nakahata, David G Warnock, Hitoshi Sakuraba, Futoshi Shibasaki   +12 more
doaj   +1 more source

Cell permeabilization of Kluyveromyces and Saccharomyces species to obtain potential biocatalysts for lactose hydrolysis

Acta Scientiarum: Biological Sciences, 2022
Yeast’s beta-galactosidase is an intracellular enzyme, through which it is possible to determine in vivo its activity as a biocatalyst in the lactose hydrolysis.
Érika de Pádua Alves, Alessandra Bosso, Luiz Rodrigo Ito Morioka, Hélio Hiroshi Suguimoto   +3 more
doaj   +1 more source

Development of a Biosimilar of Agalsidase Beta for the Treatment of Fabry Disease: Preclinical Evaluation

Drugs in R&D, 2023
Background and Objective Fabry disease (FD) is a rare lysosomal storage disorder caused by a deficiency of the enzyme α-galactosidase A (aGal A). Since 2001, two different enzyme replacement therapies have been authorized, with agalsidase beta being used
André B. P. van Kuilenburg, Carla E. M. Hollak, Ana Travella, Melisa Jacobs, Lucas D. Gentilini, René Leen, Karen M. M. Ghauharali-van der Vlugt, Femke S. Beers Stet, Susan M. I. Goorden, Sanne van der Veen, Marcelo Criscuolo, Mariana Papouchado   +11 more
doaj   +1 more source

Study of β-Galactosidase Enzyme Activity Produced by Lactobacilli in Milk and Cheese [PDF]

Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum, 2008
Background and objective Lactose intolerance is a discomfort state that occurs in some people after ingestion of milk and it is due to insufficient amount of beta galactosidase in the human gut to digest lactose.
J Nowroozi, Rahbar Roshandel, Gheytanchihandel   +2 more
doaj   +1 more source

Detection of Cellular Senescence in Human Primary Melanocytes and Malignant Melanoma Cells In Vitro

Cells, 2022
Detection and quantification of senescent cells remain difficult due to variable phenotypes and the absence of highly specific and reliable biomarkers. It is therefore widely accepted to use a combination of multiple markers and cellular characteristics ...
Tom Zimmermann, Michaela Pommer, Viola Kluge, Chafia Chiheb, Susanne Muehlich, Anja-Katrin Bosserhoff   +5 more
doaj   +1 more source

Hydrolysis of a novel lysosomotropic enzyme substrate for beta-galactosidase within intact cells.

Journal of Lipid Research, 1994
With the goal of improving the detection of lysosomal sphingolipid hydrolases within intact cells, we have recently synthesized a new fluorophor, O-[4-(1-imidazolyl)butyl]-2,3-dicyano-1,4-hydroquinonyl beta-D-galactopyranoside (Im-DCH-beta-Gal).
C R Kaneski, S A French, M R Brescia, M J Harbour, S P Miller   +4 more
doaj   +1 more source

Programmed Cell Senescence in the Mouse Developing Spinal Cord and Notochord

Frontiers in Cell and Developmental Biology, 2021
Programmed cell senescence is a cellular process that seems to contribute to embryo development, in addition to cell proliferation, migration, differentiation and programmed cell death, and has been observed in evolutionary distant organisms such as ...
Jorge Antolio Domínguez-Bautista, Pilar Sarah Acevo-Rodríguez, Susana Castro-Obregón   +2 more
doaj   +1 more source

Isolation and Identification of an α-Galactosidase-Producing Lactosphaera pasteurii Strain and Its Enzymatic Expression Analysis

Molecules, 2022
α-Galactosidase (EC 3.2.1.22) refers to a group of enzymes that hydrolyze oligosaccharides containing α-galactoside-banded glycosides, such as stachyose, raffinose, and verbascose.
Yan Zhao, Jinghui Zhou, Shan Dai, Xiaozhu Liu, Xuewen Zhang   +4 more
doaj   +1 more source