Results 251 to 260 of about 76,944 (297)
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[Immobolization of beta-galactosidase on silochrome].
Prikladnaia biokhimiia i mikrobiologiia, 1977Beta-Galactosidase (beta-D-galactoside galactohydrolase 3.2.1.23) from Curvularia inaequalis was immobilized by glutaric dialdehyde on gamma-aminopropyl triethoxysilane treated porous siliceous carrier silochrome. From the crude preparation with a specific activity of 3.1 U/mg immobilized beta-galactosidase with an activity of 113 U/g was obtained. The
K E, Pappel' +3 more
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Immobilization of mold beta-galactosidase
Collection of Czechoslovak Chemical Communications, 1988We immobilized beta-galactosidase (EC 3.2.1.23) from the mold Aspergillus oryzae by various methods on the derivatives of bead cellulose manufactured by Czechoslovak industry. The activity, specific activity and operational stability of the immobilized enzyme were determined.
Milena Kmínková +2 more
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Journal of biochemistry, 1981
Beta-Galactosidase was partially restored by protease inhibitors, leupeptin, chymostatin and E-64 in cultured fibroblasts from three patients with beta-galactosidase-neuraminidase deficiency. Pepstatin did not activate this enzyme. Neuraminidase was not affected by any of these compounds in the culture medium.
Y, Suzuki +4 more
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Beta-Galactosidase was partially restored by protease inhibitors, leupeptin, chymostatin and E-64 in cultured fibroblasts from three patients with beta-galactosidase-neuraminidase deficiency. Pepstatin did not activate this enzyme. Neuraminidase was not affected by any of these compounds in the culture medium.
Y, Suzuki +4 more
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A dimer-dimer binding region in .beta.-galactosidase
Biochemistry, 1979alpha Complementation in beta-galactosidase is the restoration of enzyme activity by addition of the alpha donor CNBr2, from amino acid residues 3--92 of the polypeptide, to inactive M15 protein from the lacZ deletion mutant strain M15. M15 protein lacks residues 11--41 and is a dimer; the active complex, like native beta-galactosidase, is tetrameric ...
F, Celada, I, Zabin
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HEPATIC BETA GALACTOSIDASE AND FELINE GMI GANGLIOSIDOSIS
Neuropathology and Applied Neurobiology, 1981Barnes I.C., Kelly D.F., Pennock C.A. & Randell J.A.J. (1981) Neuropathology and Applied Neurobiology 7, 463–476Hepatic beta galactosidase and feline GMI gangliosidosisThis paper describes the clinical, morphological and biochemical features of three cats with a progressive neurological disorder. Clinical features were ataxia and progressive tremor.
I C, Barnes +3 more
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Cold Spring Harbor protocols, 2010
When a transient or stable transfection assay is developed for a promoter, a primary objective is to quantify promoter strength. Because transfection efficiency in such assays can be low, promoters are commonly fused to heterologous reporter genes that encode enzymes that can be quantified using highly sensitive assays.
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When a transient or stable transfection assay is developed for a promoter, a primary objective is to quantify promoter strength. Because transfection efficiency in such assays can be low, promoters are commonly fused to heterologous reporter genes that encode enzymes that can be quantified using highly sensitive assays.
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Biochemistry international, 1984
beta-Galactosidase was normalized by a serine-thiol protease inhibitor, leupeptin with concentration of 10 micrograms/ml in cultured skin fibroblasts from patients with beta-galactosidase-alpha-neuraminidase deficiency (beta-Gal-/Neu-). The induction of this enzyme was not observed in normal cells. Because the enzymic activity of cathepsin B1 increased
T, Kato +3 more
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beta-Galactosidase was normalized by a serine-thiol protease inhibitor, leupeptin with concentration of 10 micrograms/ml in cultured skin fibroblasts from patients with beta-galactosidase-alpha-neuraminidase deficiency (beta-Gal-/Neu-). The induction of this enzyme was not observed in normal cells. Because the enzymic activity of cathepsin B1 increased
T, Kato +3 more
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[Modification and immobilization of beta-galactosidase].
Biokhimiia (Moscow, Russia), 1976beta-galactosidase from fungus Curvularia inaequalis was modified by a chlortriazin dye active bright-orange KH. The modified enzyme contained two molecules of dye per one molecule of protein. The incorporation of six sulfuric groups with remains of the dye resulted in a slight decrease of the acid protein isoelectric point.
V S, Zolotova +3 more
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[Research of beta-galactosidase in the leptospira].
Annali Sclavo; rivista di microbiologia e di immunologia, 1976The Authors have investigated on the presence of beta-galactoxidase in the serotypes of Leptospires most frequently employed for the diagnosis of the formes epidemiologic occurrent in Italy. With the two employed methodes it was possible to make evident the enzyme in all the serotypes, but not in L. saxkoebing (Mus 24); L.
F, Canazi, D, Crupi, F, Quartarone
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Beta-Galactosidase of Shigella Sonnei
Nature, 1963C R, CLAUSEN, M, NAKAMURA
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