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Three cases of beta-galactosidase deficiency
Klinische Pädiatrie, 1981A clinical description of three cases of beta-galactosidase deficiency is presented. Two cases are classical for infant type GM1-gangliosidosis, the third is characterised by dysostosis multiplex with growth retardation and of normal intelligence. Laboratory data revealed mucopolysaccharides in the urine of all described patients in a high level of ...
E, Pronicka +3 more
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Beta-galactosidase from Aureobasidium pullulans
Letters in Applied Microbiology, 1989Aureobasidium pullulans is a new source of enzyme beta-galactosidase with optimum activity at pH 6·8 and a temperature of 45°C. Lactose induced enzyme synthesis, but low constitutive levels of enzyme were seen with other sugars. Addition of Mn2+ and Ca2+ increased enzyme production and activity. Co2+ was a strong activator of enzyme activity.
M.S. Deshpande +2 more
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Beta-Galactosidase Staining in the Skeleton
2014The lacZ gene, encoding for the β-galactosidase enzyme, is widely used as a reporter gene in bone biology due to the ease of visualization in situ on whole-mount or on tissue sections. In this protocol we provide detailed methods for visualizing this reporter gene for both in vivo and in vitro studies.
X L, Han, Jian Q, Feng
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Immobilization of mold beta-galactosidase
Collection of Czechoslovak Chemical Communications, 1988We immobilized beta-galactosidase (EC 3.2.1.23) from the mold Aspergillus oryzae by various methods on the derivatives of bead cellulose manufactured by Czechoslovak industry. The activity, specific activity and operational stability of the immobilized enzyme were determined.
Milena Kmínková +2 more
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HEPATIC BETA GALACTOSIDASE AND FELINE GMI GANGLIOSIDOSIS
Neuropathology and Applied Neurobiology, 1981Barnes I.C., Kelly D.F., Pennock C.A. & Randell J.A.J. (1981) Neuropathology and Applied Neurobiology 7, 463–476Hepatic beta galactosidase and feline GMI gangliosidosisThis paper describes the clinical, morphological and biochemical features of three cats with a progressive neurological disorder. Clinical features were ataxia and progressive tremor.
I C, Barnes +3 more
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Journal of biochemistry, 1981
Beta-Galactosidase was partially restored by protease inhibitors, leupeptin, chymostatin and E-64 in cultured fibroblasts from three patients with beta-galactosidase-neuraminidase deficiency. Pepstatin did not activate this enzyme. Neuraminidase was not affected by any of these compounds in the culture medium.
Y, Suzuki +4 more
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Beta-Galactosidase was partially restored by protease inhibitors, leupeptin, chymostatin and E-64 in cultured fibroblasts from three patients with beta-galactosidase-neuraminidase deficiency. Pepstatin did not activate this enzyme. Neuraminidase was not affected by any of these compounds in the culture medium.
Y, Suzuki +4 more
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Cold Spring Harbor protocols, 2010
When a transient or stable transfection assay is developed for a promoter, a primary objective is to quantify promoter strength. Because transfection efficiency in such assays can be low, promoters are commonly fused to heterologous reporter genes that encode enzymes that can be quantified using highly sensitive assays.
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When a transient or stable transfection assay is developed for a promoter, a primary objective is to quantify promoter strength. Because transfection efficiency in such assays can be low, promoters are commonly fused to heterologous reporter genes that encode enzymes that can be quantified using highly sensitive assays.
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Endo-beta-galactosidases and keratanase.
Current protocols in molecular biology, 2008This overview covers the endo-beta-galactosidases; enzyme is capable of hydrolyzing a wide range of glycoconjugates. Endo-beta-galactosidases from numerous sources are discussed in terms of their substrate specificities and substrates, as well as their practical research applications.
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Rabbit small intestinal beta-galactosidases.
American journal of veterinary research, 1984The beta-galactosidase activities of the rabbit small intestinal mucosa were studied over the pH range of 2.6 to 8.4, using different substrates, and in the presence or absence of the enzyme inhibitor p-chloromercuribenzoic acid. The results indicated the presence of 4 beta-galactosidases: (i) a neutral beta-galactosidase (lactase) with optimum pH of 5.
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