Results 51 to 60 of about 7,983 (189)

Synthetic polymer nanocarrier enhances dsRNA stability but not RNAi efficacy in Brassicogethes aeneus

Pest Management Science, EarlyView.
Experimental setup for dsRNA feeding in Brassicogethes aeneus to evaluate the effect of a synthetic polymer nanocarrier on RNAi efficiency. The experiment assessed whether the nanocarrier enhances RNAi after oral dsRNA delivery. (a) Stability of dsRNA–nanocarrier complexes was tested in simulated gut conditions. (b) Gene expression of αCOP and dre4 was
Triin Kallavus, Liina Soonvald, Iris Callewaert, Ana Isabel Silva, Ericmar Ávila Dos Santos, Silva Vilumets, Riina Kaasik, Sven Vereecken, Lenny Van Daele, Peter Dubruel, Jonathan Willow, Clauvis Nji Tizi Taning, Kristof De Schutter, Eve Veromann   +13 more
wiley   +1 more source

TcALS knockdown accelerates metamorphosis via insulin signaling in Tribolium castaneum

Insect Science, EarlyView.
RNAi silencing of TcALS accelerates larva‐to‐pupa metamorphosis in Tribolium castaneum. Abstract Insect metamorphosis is an evolutionary adaptation precisely regulated by nutritional and hormonal cues. The red flour beetle Tribolium castaneum, a major stored‐product pest, serves as an excellent model for studying this process.
Min Li, Jie Chen, Zi‐Ye Wang, Fei‐Feng Wang, Lu‐Lu Dong, Jun Pan, Min‐Er Li, Lin Yu, Wen Sang   +8 more
wiley   +1 more source

Large differences in carbohydrate degradation and transport potential among lichen fungal symbionts. [PDF]

, 2022
Lichen symbioses are thought to be stabilized by the transfer of fixed carbon from a photosynthesizing symbiont to a fungus. In other fungal symbioses, carbohydrate subsidies correlate with reductions in plant cell wall-degrading enzymes, but whether ...
Andresson OS, Bujold AR, Fernandez-Brime S, Freire Rallo S, Guomundsson H, Kono M, Mayrhofer H, McCutcheon J, Meidl P, Muggia L, Resl P, Spribille T, Tagirdzhanova G, Wedin M, Werth S, Willis LM   +15 more
core   +1 more source

The Diagnostic Odyssey of a Biochemically Confirmed Case of ML II: The First Western Patient With LYSET Deficiency

Clinical Genetics, EarlyView.
We identify a female patient with a homozygous nonsense variant (p.Gln38Ter) in the LYSET gene. This is the first western report of a challenging case of an extensive diagnostic odyssey and demonstrates that the LYSET gene must be considered in the differential diagnosis when M6P‐labeled lysosomal enzymes are altered.
Fernanda Sperb‐Ludwig, Taciane Alegra, Leonardo Martinello da Rosa, Nataniel Floriano Ludwig, Renata Voltolini Velho, Ida Vanessa Doederlein Schwartz   +5 more
wiley   +1 more source

Endo-.BETA.-Mannosidase, a Plant Enzyme Acting on N-Glycans

Trends in Glycoscience and Glycotechnology, 2006
N-配糖体のコア構造中のManβ1-4GlcNAc結合を加水分解するエンド-β-マンノシダーゼ(EC3.1.2.152) が高等植物より見いだされた。我々は、最近、エンド-β-マンノシダーゼの精製、遺伝子クローニングに成功した。エンド-β-マンノシダーゼのオーソログ遺伝子は植物種のみからしか見いだせず、この酵素は植物に特異的な機能をもっていると考えられる。このエンドグリコシダーゼの基質特異性は、(Man)nManα1-6Manβ1-4GlcNAcβ1-4GlcNAc (n=0～2)のManβ1-4GlcNAc結合を加水分解するというユニークなものである。これらの基質はManα1-3Manβ結合を優先的に加水分解するタチナタマメα-マンノシダーゼ様酵素がハイマンノース型糖鎖に作用することによって生じる。
Takeshi Ishimizu, Sumihiro Hase
openaire   +2 more sources

Molecular Cloning and Characterization of Bovine β-Mannosidase [PDF]

Journal of Biological Chemistry, 1995
Deficiency of lysosomal beta-mannosidase activity results in a severe neurodegenerative disease in goats and cattle and a relatively milder phenotype in humans. A cDNA coding for the entire beta-mannosidase protein is described. Mixed oligonucleotides derived from bovine beta-mannosidase peptide sequences were used to screen a bovine thyroid cDNA ...
H, Chen, J R, Leipprandt, C E, Traviss, B L, Sopher, M Z, Jones, K T, Cavanagh, K H, Friderici   +6 more
openaire   +2 more sources

Cryo‐EM structure of GH43 β‐Xylosidase from Enterobacter cloacae provides insights into substrate specificity and the role of an auxiliary domain in enzymatic activity

The FEBS Journal, EarlyView.
Xylan, the second most abundant plant cell wall polysaccharide, is degraded by β‐xylanases and β‐xylosidases. Here, we present the 2.65 Å cryo‐EM structure of Enterobacter cloacae β‐xylosidase (EcXyl43, GH43) and the 2.4 Å X‐ray structure of its inactive F507A mutant.
Lorenzo Briganti, Andre Schutzer Godoy, Caio Cesar Mello Capetti, Rafaela Sachetto Fernandes, Vanessa O. A. Pellegrini, Ornella M. Ontañon, Eleonora Campos, Rodrigo V. Portugal, Igor Polikarpov   +8 more
wiley   +1 more source

Solitary angiokeratoma of the tongue [PDF]

, 2008
Angiokeratoma is a rare cutaneous lesion. It can be either a generalized systemic form, pesenting as multiple asymptomatic papules on the skin, associated with metabolic diseases or a solitary cutaneous form.
Bodner, Lipa, Manor, Esther, Puterman, Max, Sion Vardy, Netta   +3 more
core  

An in vitro model of murine middle ear epithelium. [PDF]

, 2016
Otitis media (OM) or middle ear inflammation is the most common pediatric disease and leads to significant morbidity. Although understanding of underlying disease mechanisms is hampered by complex pathophysiology it is clear that epithelial abnormalities
Admyre, Amesara, Atherton, Bakaletz, Barnes, Bartlett, Bernstein, Bingle, Butler, Casey, Cash, Choi, Choi, Chun, Clarke, Cody, Davidson, Davies, Gao, Gaschler, Hendrixson, Herman, Horani, Horani, Juhn, Kapsogeorgou, Keller, Kerschner, Kesimer, Kim, Kondo, Lechner, Lim, Lim, Lim, Lim, Liu, Loonen, Matsui, Mcguire, Moon, Moon, Musa, Nakamura, Pillai, Portier, Preciado, Preciado, Roy, Rye, Rye, Schachern, Schousboe, Straetemans, Takeno, Thompson, Toyama, Tsuchiya, Ueyama, Vaishnava, Val, Valadi, Van Blitterswijk, Van Niel, van Rozendaal, Vladar, Woo, Woodfield, Woodworth, Wright, Wu, Yamaya, You, You   +73 more
core   +3 more sources

A Practical and Flexible De Novo Synthesis of Deoxygenated Carbasugars and Their Cyclitol Epoxides

European Journal of Organic Chemistry, Volume 29, Issue 18, 14 May 2026.
A de novo synthesis route comprising enantioselective Brown crotylation and ring‐closing metathesis as the key steps enables the synthesis of a‐ and b‐carba‐paratose, a‐ and b‐carba‐colitose as well as four configurational deoxycyclophellitol isosteres.
Yevhenii Radchenko, Nick D. F. Puijmbroeck, Sanne M. Verduin, Jeroen D. C. Codée, Herman S. Overkleeft   +4 more
wiley   +1 more source