Results 151 to 160 of about 144,132 (182)

The inhibition of β-N-acetylhexosaminidase by lactones

Biochimica et Biophysica Acta (BBA) - Enzymology, 1973
β-N-Acetylhexosaminidase (2-acetamido-2-deoxy-β-hexoside acetamidodeoxy-hexohydrolase, EC 3.2.1.52), from commercial bovine serum albumin, was inhibited by glucoheptonolactone, d- and l-gluconolactone, d-galactonolactone, d-fuconolactone, l-arabonolactone, d-ribonolactone, l-mannolactone and l-ascorbic acid. The kinetics of inhibition of the hydrolysis
J N, Kanfer, C H, Spielvogel
openaire   +2 more sources

Identification of an intermediate form of β-N-acetylhexosaminidase in chorionic villi

Clinica Chimica Acta, 1990
A minor form of beta-N-acetylhexosaminidase has been found in chorionic villi, in addition to the major forms A and B. This form does not hydrolyze the 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulpha te substrate, is thermostable, has a higher mol mass (120,000) than A and B (100,000) and on analytical isoelectric focusing, it ...
Pieretti M., BECCARI, Tommaso, Cosmi E. V., Stirling J. L., ORLACCHIO, Aldo   +4 more
openaire   +3 more sources

Beta-N-acetylhexosaminidase activity in human preimplantation embryos

1992
---
Sermon, Karen, Lissens, Willy, Tarlatzis, Basil, Braude, P., Devroey, Paul, Van Steirteghem, Andre, Liebaers, Ingeborg   +6 more
openaire   +1 more source

β-N-acetylhexosaminidase isoenzymes during chick embryo development

International Journal of Biochemistry, 1989
1. Two forms (I and II) with acidic pH optima and a neutral form of beta-hexosaminidase has been separated by DEAE-cellulose chromatography and characterized in skin and lung of 7, 9, 11, 14 day chick embryos and 1 day old chicken. 2. Forms I and II are similar to hexosaminidase A and B for their behaviour on DEAE-cellulose chromatography, Concanavalin
BECCARI, Tommaso, PEZZETTI, Furio, BELARDINELLI, Raffaela, BODO, Maria, BECCHETTI, Ennio, ORLACCHIO, Aldo   +5 more
openaire   +3 more sources

beta-N-acetylhexosaminidases from Serratia marcescens.

Microbios, 1992
Two forms of beta-N-acetylhexosaminidase from Serratia marcescens with an optimum pH of 5.0 and 6.5, respectively, to 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside were separated by DEAE-cellulose chromatography and Sephacryl S-200 chromatography.
CENCI, Giovanni, BECCARI, Tommaso, CALDINI, Giovanna, G. Bellachioma, ORLACCHIO, Aldo   +4 more
openaire   +2 more sources

Characterization of a beta-N-acetylhexosaminidase and a beta-N-acetylglucosaminidase/beta-glucosidase from Cellulomonas fimi.

The FEBS journal, 2006
The gram-positive soil bacterium Cellulomonas fimi is shown to produce at least two intracellular beta-N-acetylglucosaminidases, a family 20 beta-N-acetylhexosaminidase (Hex20), and a novel family 3-beta-N-acetylglucosaminidase/beta-glucosidase (Nag3), through screening of a genomic expression library, cloning of genes and analysis of their sequences ...
Christoph, Mayer, David J, Vocadlo, Melanie, Mah, Karen, Rupitz, Dominik, Stoll, R A J, Warren, Stephen G, Withers   +6 more
openaire   +2 more sources

[Screening of beta-N-acetylhexosaminidase forming strains and conditions for enzyme production].

Wei sheng wu xue bao = Acta microbiologica Sinica, 1990
About 1200 strains of microorganisms were screened including fungi, actinomyces, and bacteria, in which 237 strains producing the enzyme desired. The results showed that the beta-GlcNAcase and beta-GalNAcase always co-existed in one strain, though may be in different ratio.
Z Z, Yan, Y, Tao, X L, Cheng, J W, Sun, S Z, Zhang   +4 more
openaire   +1 more source

Beta-N-Acetylhexosaminidases accept 4-deoxy-N-acetylhexosaminides as substrates

2009
b-N-Acetylhexosaminidases (EC 3.2.1.52, CAZy GH20) possess so called wobbling specificity, which means that they cleave substrates both in gluco- and galacto- configurations, with the activity ratio depending on the enzyme source. Here we present a new finding that fungal b-N-acetylhexosaminidases are also able to hydrolyze and transfer 4-deoxy-N ...
Slamova K, Bojarova P, Gazak R, Tramice A, Kulik N, Mackova M, Kren V   +6 more
openaire   +2 more sources

pH-sensitive dissociation and association of beta-N-acetylhexosaminidase from boar sperm acrosome.

Biology of reproduction, 1994
beta-N-Acetylhexosaminidase (beta-Hex, EC, 3.2.1.52) was released from cauda epididymal boar sperm by treatment with ionophore A23187, indicating that this enzyme is localized in the acrosome. beta-Hex was extracted on a large scale, with 2% acetic acid containing 0.2% Brij 35, from washed ejaculated sperm.
M, Takada, N, Yonezawa, M, Yoshizawa, S, Noguchi, Y, Hatanaka, T, Nagai, K, Kikuchi, H, Aoki, M, Nakano   +8 more
openaire   +1 more source

Large propeptides of fungal beta-N-acetylhexosaminidases are novel enzyme regulators that must be intracellularly processed to control activity, dimerization, and secretion into the extracellular environment.

Biochemistry, 2007
O. Plíhal, J. Skłenar, K. Hofbauerová, P. Novák, P. Man, P. Pompach, D. Kavan, H. Ryšlavá, L. Weignerová, Andrea Charvátová-Pišvejcová, V. Křen, K. Bezouška   +11 more
semanticscholar   +1 more source