Results 211 to 220 of about 253,228 (248)

TESTOSTERONE ASSAYS BY COMPETITIVE PROTEIN BINDING

Acta Endocrinologica, 1970
ABSTRACT A review of competitive protein binding methods for the determination of testosterone in plasma is given. The different steps discussed are: Choice of the binding protein, dilution of the protein solution, separation of the free from the bound fraction, purification of the extract and factors determining the blank.
A, Vermeulen, L, Verdonck
openaire   +2 more sources

Competitive protein binding assay of corticosterone

Journal of Steroid Biochemistry, 1972
Abstract Details of a competitive protein binding assay of corticosterone are described. The preliminary TLC separation permits simultaneous estimation of corticosterone, aldosterone and progesterone. “Unstressed” rat plasma corticosterone values were obtained between 0.3 and 9 μg/ 100 ml.
A, Spät, S, Józan
openaire   +2 more sources

Competitive Protein Binding Assay for Piritrexim

Journal of Pharmaceutical Sciences, 1989
A competitive protein binding assay for piritrexim (PTX, 1) that makes use of a commercially available radioassay kit for methotrexate has been developed. After it is selectively extracted from plasma, PTX competes with [125l]methotrexate for binding to dihydrofolate reductase isolated from Lactobacillus casei. Free drug is separated from bound drug by
J L, Woolley, J L, Ringstad, C W, Sigel
openaire   +2 more sources

Competitive protein binding assay for 24,25-dihydroxycholecalciferol

Biochemical and Biophysical Research Communications, 1976
Abstract A competitive protein binding assay which measures 24,25-dihydroxycholecalciferol in human serum has been developed using the binding protein from vitamin D-deficient rat kidney. As 25-hydroxycholecalciferol and 25,26-dihydroxycholecalciferol also interact with the binding protein, possible interference by these compounds in the assay has ...
C M, Taylor, S E, Hughes, P, de Silva
openaire   +3 more sources

Competitive binding assays for riboflavin and riboflavin-binding protein

Analytical Biochemistry, 1982
Abstract A competitive binding procedure that can be used to determine either riboflavin or riboflavin-binding protein has been developed. Riboflavin-binding protein from chicken egg white binds tightly to DEAE-cellulose while free riboflavin does not.
S E, Lotter, M S, Miller, R C, Bruch, H B, White   +3 more
openaire   +2 more sources

Competitive hapten-antibody binding: Solution of the binding equation

Journal of Theoretical Biology, 1965
Abstract The competitive binding equation which describes the reactions between two univalent homogeneous cross-reacting haptens and antibody heterogeneous with respect to equilibrium constants has been solved. The joint probability density function of equilibrium constants can be computed directly from binding measurements; no assumption about the ...
J, Wong, F, Aladjem
openaire   +2 more sources

Analyzing competitive binding data

2004
Abstract Competitive binding experiments measure the binding of a single concentration of labeled ligand in the presence of various concentrations of unlabeled ligand. Ideally, the concentration of unlabeled ligand varies over at least six orders of magnitude.
Harvey Motulsky, Arthur Christopoulos
openaire   +1 more source

Homologous competitive binding curves

2004
Abstract The most common way to determine receptor number ,, nd affinity is to perform a saturation binding experiment, where you vary the concentration of radioligand. An alternative is to keep the radioligand concentration constant, and compete for binding with the same ligand, but not radioactively labeled. Since the hot (radiolabeled)
Harvey Motulsky, Arthur Christopoulos
openaire   +1 more source

Bargaining, binding contracts, and competitive wages

Games and Economic Behavior, 2003
In a model where many workers bargain with one firm and sign binding contracts, the existence of a stationary subgame perfect equilibrium is shown. If the production function satisfies decreasing returns, each worker receives a share of his marginal product (treating all other workers as employed) in equilibrium. Thus, wages are competitive. This is in
openaire   +2 more sources

Adrenocorticotropin Measurement by Competitive Binding Receptor Assay

The Journal of Clinical Endocrinology & Metabolism, 1972
A competitive binding assay using the cortical receptor from normal adrenal glands has been developed for ACTH. In this assay porcine, human or rabbit ACTH has been shown to compete with labeled porcine ACTH for the cortical receptor from ovine, rabbit or rat adrenal glands.
A R, Wolfsen, H B, McIntyre, W D, Odell
openaire   +2 more sources