A highly sensitive bead-based flow cytometric competitive binding assay to detect SARS-CoV-2 neutralizing antibody activity [PDF]
Frontiers in Immunology, 2022 Accurate detection of SARS-CoV-2 neutralizing antibody (nAb) is critical for assessing the immunity levels after virus infection or vaccination. As fast, cost-effective alternatives to viral infection-based assays, competitive binding (CB) assays were ...
Xiangyu Yao +17 more
doaj +2 more sources
A competitive binding-mass spectrometry strategy for high-throughput evaluation of potential critical quality attributes of therapeutic monoclonal antibodies [PDF]
mAbs, 2022 Therapeutic monoclonal antibodies (mAbs) have a propensity to host a large number of chemical and enzymatical modifications that need to be properly assessed for their potential impact on target binding.
Zhengqi Zhang +3 more
doaj +2 more sources
Competitive dCas9 binding as a mechanism for transcriptional control [PDF]
Molecular Systems Biology, 2021 Catalytically dead Cas9 (dCas9) is a programmable transcription factor that can be targeted to promoters through the design of small guide RNAs (sgRNAs), where it can function as an activator or repressor.
Daniel A Anderson, Christopher A Voigt
doaj +4 more sources
bindNode24: Competitive binding residue prediction with 60 % smaller model [PDF]
Computational and Structural Biotechnology JournalMany proteins function through ligand binding. Yet, reliable experimental binding data remains limited. Recent advances predict binding residues from sequences using protein Language Model embeddings.
Kyra Erckert +2 more
doaj +2 more sources
Probiotics mitigate non-antibiotic drug-induced dysbiosis via protein homology-driven competitive binding [PDF]
Nature CommunicationsWhile the impact of non-antibiotic drugs on gut bacteria is well-known, their mechanisms of action remain poorly characterized, and effective mitigation strategies for drug-induced dysbiosis are still limited.
Shuo Feng +20 more
doaj +2 more sources
CLASP-mediated competitive binding in protein condensates directs microtubule growth [PDF]
Nature CommunicationsMicrotubule organization in cells relies on targeting mechanisms. Cytoplasmic linker proteins (CLIPs) and CLIP-associated proteins (CLASPs) are key regulators of microtubule organization, yet the underlying mechanisms remain elusive. Here, we reveal that
Xuanyan Jia +11 more
doaj +2 more sources
mBio, 2022 Although the phosphorothioate (PT) modification, in which the nonbridging oxygen in the DNA sugar-phosphate backbone is replaced by sulfur, has been reported to play versatile roles in multiple cellular processes, very little data have been obtained to ...
You Tang +10 more
doaj +1 more source
A Polarity-Sensitive Far-Red Fluorescent Probe for Glucose Sensing through Skin
Biosensors, 2023 The field of glucose biosensors for diabetes management has been of great interest over the past 60 years. Continuous glucose monitoring (CGM) is important to continuously track the glucose level to provide better management of the disease.
Lydia Colvin +4 more
doaj +1 more source
Validating a re-implementation of an algorithm to integrate transcriptome and ChIP-seq data [PDF]
PeerJ, 2023 Transcription factor binding to a gene regulatory region induces or represses its expression. Binding and expression target analysis (BETA) integrates the binding and gene expression data to predict this function.
Mahmoud Ahmed, Deok Ryong Kim
doaj +2 more sources
Journal of Functional Biomaterials, 2023 Currently available hemodialysis (HD) membranes are unable to safely remove protein-bound uremic toxins (PBUTs), especially those bonded to human serum albumin (HSA).
Madalena Lopes +3 more
doaj +1 more source