Results 71 to 80 of about 1,041,210 (312)

Enhancing Binding Affinity of an Intrinsically Disordered Protein by α-Methylation of Key Amino Acid Residues [PDF]

, 2019
Intrinsically disordered proteins (IDPs), which undergo folding upon binding to their targets, are critical players in protein interaction networks.
Gilles, Travé, Natacha, Rochel, Vladimir, Torbeev, Camille, Kostmann, Bruno, Kieffer, Alexandra, Cousido-Siah, Boris, Schmidtgall, Gergő, Gógl, André, Mitschler, Judit, Osz, Jozica, Dolenc, Valentin, Bauer, Yves, Nominé   +12 more
core   +1 more source

Organizing the interface—Plasma membrane architecture and receptor dynamics in virus‐cell interactions

FEBS Letters, EarlyView.
Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley   +1 more source

Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations

FEBS Letters, EarlyView.
The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas, Brigitta Maruzs, Zsófia E. Kálmán, Tomas Klumpler, Gyula Batta, Bálint Péterfia, Zoltán Gáspári   +6 more
wiley   +1 more source

An albumin-derived peptide scaffold capable of binding and catalysis [PDF]

, 2012
We have identified a 101-amino-acid polypeptide derived from the sequence surrounding the IIA binding site of human albumin. The polypeptide contains residues that make contact with ligands as warfarin in the parent protein, and eight cysteine residues ...
SBLATTERO, DANIELE, Fabio Benedetti, Giampaolo Fontanive (381542), SGARRA, RICCARDO, Daniele Sblattero (251886), Riccardo Sgarra (103906), Immacolata Luisi (381540), MAURIZIO, ELISA, Silvia Pavan (381541), Fabio Benedetti (243799), BENEDETTI, FABIO, Adriano Savoini, LUISI, Immacolata, Alessandro Tossi, Federico Berti, Immacolata Luisi, FONTANIVE, GIAMPAOLO, Silvia Pavan, Federico Berti (381545), BERTI, FEDERICO, Alessandro Tossi (381543), Elisa Maurizio, Ro Tossi, Adriano Savoini (381544), TOSSI, ALESSANDRO, Daniele Sblattero, Savoini A., PAVAN, SILVIA, Riccardo Sgarra, Elisa Maurizio (103901), Giampaolo Fontanive   +30 more
core   +2 more sources

An unexpected alternative viologen electron mediator site in tungsten‐containing formate dehydrogenase

FEBS Letters, EarlyView.
An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley   +1 more source

DrugForm-DTA: Towards real-world drug-target binding affinity model

Computational and Structural Biotechnology Journal
Drug-target affinity (DTA) prediction is a fundamental challenge in drug discovery. Computational methods for predicting DTA can greatly assist drug design by narrowing the search space and reducing the number of protein-ligand complexes with low ...
Ivan Khokhlov, Anna Tashchilova, Nikolai Bugaev-Makarovskiy, Olga Glushkova, Vladimir Yudin, Anton Keskinov, Sergey Yudin, Dmitry Svetlichnyy, Veronika Skvortsova   +8 more
doaj   +1 more source

Phosphoinositides and inositol phosphates as molecular glues

FEBS Letters, EarlyView.
Inositol phosphates (IPs) and phosphoinositides (PIPs) regulate diverse eukaryotic processes. Beyond recruiting signaling proteins or acting as structural cofactors, recent studies suggest they mediate protein–protein interactions as natural molecular glues.
Aleshia Seaton‐Terry, Alexander J. Cutright, Mong Na Claire Loi, Jessica Martin, Joseph W. Gilligan, Jakub N. Kubina, Michael J. Arambula, Susanna Tanada Palmu, Daniella Zhou, Raymond D. Blind   +9 more
wiley   +1 more source

Binding mechanism of affinity ligands for purification of plasmid DNA [PDF]

, 2007
Plasmid DNA for therapeutic and vaccination purposes must be highly purified. The high selectivity of affinity chromatography makes it ideal for the isolation of pDNA from complex biological feed stocks.
Forde, G.M., Ying, H.
core  

Affinity- and Format-Dependent Pharmacokinetics of ⁸⁹Zr-Labeled Albumin-Binding VHH Constructs

Pharmaceuticals
Background/Objectives: NANOBODY® molecules (VHHs) are attractive vectors for radiopharmaceuticals due to their small size and high target affinity, but rapid clearance and pronounced kidney retention limit their therapeutic applicability.
Simon Leekens, Peter Casteels, Tom Van Bogaert, Pieter Deschaght, Veronique De Brabandere, Christopher Cawthorne, Guy Bormans, Frederik Cleeren   +7 more
doaj   +1 more source

Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis

FEBS Letters, EarlyView.
Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee, Junho Jeong, Chae Yeong Lee, Hyunjoon Cho, Man Hwan Oh, Je Chul Lee, Jee‐Young Lee, Yangmee Kim   +7 more
wiley   +1 more source