Results 291 to 300 of about 3,018,689 (332)
Some of the next articles are maybe not open access.
Nature Methods, 2021
KIN-CLIP allows determination of binding and dissociation kinetics of RNA-binding proteins in cells.
openaire +2 more sources
KIN-CLIP allows determination of binding and dissociation kinetics of RNA-binding proteins in cells.
openaire +2 more sources
Kinetics of quinine-deuterohemin binding
Biophysical Chemistry, 1993The interaction of quinine with free hemin is of importance for the antimalarial effect of the drug in infected erythrocytes. We have investigated the kinetics of the complex formation of quinine with deuterohemin using the temperature jump relaxation method.
Y, Gushimana +3 more
openaire +2 more sources
Statistical comparison of ligand‐binding kinetics
Statistics in Medicine, 1989AbstractWe describe the application of generalized linear model methodology to the problem of testing differences among ligand‐receptor interactions, and show that the method is analogous to weighted least squares regression methodology and F tests familiar to many investigators.
J B, Cologne +2 more
openaire +2 more sources
Tracing Kinetic Intermediates during Ligand Binding
Journal of the American Chemical Society, 2004Specific protein-ligand interactions are central to biological control. Although structure determination provides important insight into these interactions, it does not address dynamic events that occur during binding. While many biophysical techniques can provide a global view of these dynamics, NMR can be used to derive site-specific dynamics at ...
Tanja, Mittag +2 more
openaire +2 more sources
Measurements of aptamer-protein binding kinetics using graphene field-effect transistors.
Nanoscale, 2019Quantifying interactions between biomolecules subject to various environmental conditions is essential for applications such as drug discovery and precision medicine.
Xuejun Wang +4 more
semanticscholar +1 more source
Kinetics of calmodulin binding to calcineurin
Biochemical and Biophysical Research Communications, 2005Calcineurin (CaN) binds Ca(2+)-saturated calmodulin (CaM) with relatively high affinity; however, an accurate steady-state K(d) value has not been determined. In this report, we describe, using steady-state and stopped-flow fluorescence techniques, the rates of association and dissociation of Ca(2+)-saturated CaM from CaN heterodimer (CaNA/CaNB) and ...
Andrea R, Quintana +3 more
openaire +2 more sources
Kinetic Approach to Drug-Protein Binding
Journal of Pharmaceutical Sciences, 1974Since estimates in the literature for the fundamental binding parameters of dicumarol to bovine serum albumin show wide disparity, these phenomena were reinvestigated, using a nonequilibrium partitioning technique. A three-phase system was used consisting of an aqueous drug-protein phase and a separate aqueous receiving phase, both in mutual contact ...
J S, Robertson, B W, Madsen
openaire +2 more sources
Binding kinetics of harmonically confined random walkers
Physical Review E, 2022Diffusion-mediated binding of molecules under the influence of discrete spatially confining potentials is a commonly encountered scenario in systems subjected to explicit fields or implicit fields arising from tethering restraints. Here, we derive analytical expressions for the mean binding time of two random walkers geometrically confined by means of ...
Sebastian Sensale +2 more
openaire +2 more sources
Kinetic determination of talin-actin binding
Biochemical and Biophysical Research Communications, 1991Smooth muscle talin prepared from chicken gizzard binds to skeletal muscle actin in vitro. The stoichiometry of 1:3 for talin:fluorescent labelled G-actin was confirmed by steady state titration and viscosity measurements under non-polymerizing conditions.
W H, Goldmann, G, Isenberg
openaire +2 more sources