Results 31 to 40 of about 3,018,689 (332)
Low-affinity integrin states have faster ligand-binding kinetics than the high-affinity state
eLife, 2021 Integrin conformational ensembles contain two low-affinity states, bent-closed and extended-closed, and an active, high-affinity, extended-open state. It is widely thought that integrins must be activated before they bind ligand; however, one model holds
Jing Li, Jiabin Yan, Timothy A Springer
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Conformation-controlled binding kinetics of antibodies [PDF]
Scientific Reports, 2016 AbstractAntibodies are large, extremely flexible molecules, whose internal dynamics is certainly key to their astounding ability to bind antigens of all sizes, from small hormones to giant viruses. In this paper, we build a shape-based coarse-grained model of IgG molecules and show that it can be used to generate 3D conformations in agreement with ...
GALANTI, MARTA +2 more
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Biomolecules, 2019 Many compounds with good inhibitory activity (i.e., high affinity) within in vitro experiments failed in vivo studies due to a lack of efficacy from limited target occupancy (TO) in the drug discovery process.
Guopeng Wang +7 more
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Biosensors, 2022 The global pandemic of COVID-19 has created an unrivalled need for sensitive and rapid point-of-care testing (POCT) methods for the detection of infectious viruses.
Ting-Chou Chang +5 more
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Bivalent binding on cells varies between anti-CD20 antibodies and is dose-dependent
mAbs, 2020 Based on their mechanism of action, two types of anti-CD20 antibodies are distinguished: Type I, which efficiently mediate complement-dependent cytotoxicity, and Type II, which instead are more efficient in inducing direct cell death.
Sina Bondza +4 more
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Eng, 2023 In the quest for the development of accurate, reliable, and cost-effective biosensing technology for early diagnostics of prostate cancer, we describe here an electrochemical biosensor combining a simple transducing method of differential pulse ...
Sarra Takita +4 more
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Frontiers in Immunology, 2022 BackgroundIgG4 anbibodies are deficient in stability and may contribute to tumor-associated escape from immune surveillance. We developed an IgG1 backbone anti-programmed cell death protein-1 (PD-1) antibody, penpulimab, which is designed to remove ...
Zhaoliang Huang +10 more
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Dynamic Docking: A Paradigm Shift in Computational Drug Discovery
Molecules, 2017 Molecular docking is the methodology of choice for studying in silico protein-ligand binding and for prioritizing compounds to discover new lead candidates.
Dario Gioia +4 more
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Biomimetic Models to Investigate Membrane Biophysics Affecting Lipid–Protein Interaction
Frontiers in Bioengineering and Biotechnology, 2020 Biological membranes are highly dynamic in their ability to orchestrate vital mechanisms including cellular protection, organelle compartmentalization, cellular biomechanics, nutrient transport, molecular/enzymatic recognition, and membrane fusion ...
Joe Sarkis, Joe Sarkis, Véronique Vié
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An Advanced Automated Patch Clamp Protocol Design to Investigate Drug—Ion Channel Binding Dynamics
Frontiers in Pharmacology, 2021 Standard high throughput screening projects using automated patch-clamp instruments often fail to grasp essential details of the mechanism of action, such as binding/unbinding dynamics and modulation of gating.
Peter Lukacs +10 more
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