Results 51 to 60 of about 834,721 (279)
A Method to Study Complex Enzyme Kinetics Involving Numerical Analysis of Enzymatic Schemes. The Mannitol Permease of Escherichia coli as an Example [PDF]
, 1993 An analysis of complex kinetic mechanisms is proposed that consists of two steps, (i) building of an kinetic scheme from experimental data other than steady-state kinetics and (ii) numerical simulation and analysis of the kinetics of the proposed scheme ...
Lolkema, Juke S.,
core +1 more source
The ATPase cycle of PcrA helicase and its coupling to translocation on DNA. [PDF]
, 2009 The superfamily 1 bacterial helicase PcrA has a role in the replication of certain plasmids, acting with the initiator protein (RepD) that binds to and nicks the double-stranded origin of replication.
Martinez-Senac, Maria M +3 more
core +1 more source
Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer
FEBS Letters, EarlyView.Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani +4 more
wiley +1 more source
Química Nova, 2014 This study aimed to evaluate β-galactosidase immobilization. For this purpose, the ionic strength of the buffer, reaction time, amount of the immobilization support, and pH were evaluated by a central composite design. Assay 8, which consisted of 1.5 mol
Anna R. C. Braga +4 more
doaj +1 more source
Biosensors, 2022 Phage display technology has been a powerful tool in peptide drug development. However, the supremacy of phage display-based peptide drug discovery is plagued by the follow-up process of peptides synthesis, which is costly and time consuming, but is ...
Runli Liang +3 more
doaj +1 more source
ATPase mechanism of the 5'-3' DNA helicase, RecD2: evidence for a pre-hydrolysis conformation change [PDF]
, 2013 The superfamily 1 helicase, RecD2, is a monomeric, bacterial enzyme with a role in DNA repair, but with 5'-3' activity unlike most enzymes from this superfamily. Rate constants were determined for steps within the ATPase cycle of RecD2 in the presence of
Bae +34 more
core +1 more source
Disordered but rhythmic—the role of intrinsic protein disorder in eukaryotic circadian timing
FEBS Letters, EarlyView.Unstructured domains known as intrinsically disordered regions (IDRs) are present in nearly every part of the eukaryotic core circadian oscillator. IDRs enable many diverse inter‐ and intramolecular interactions that support clock function. IDR conformations are highly tunable by post‐translational modifications and environmental conditions, which ...
Emery T. Usher, Jacqueline F. Pelham
wiley +1 more source
Journal of Lipid Research, 2003 Lipoprotein surface charge influences cholesteryl ester transfer protein (CETP) activity and its association with lipoproteins; however, the relationship between these events is not clear.
Richard E. Morton, Diane J. Greene
doaj +1 more source
Allosteric control of olefin isomerization kinetics via remote metal binding and its mechanochemical analysis [PDF]
, 2023 Yichen Yu +4 more
openalex +1 more source
Robustness and modularity properties of a non-covalent DNA catalytic reaction [PDF]
, 2009 The biophysics of nucleic acid hybridization and strand displacement have been used for the rational design of a number of nanoscale structures and functions. Recently, molecular amplification methods have been developed in the form of non-covalent DNA
Aldaye +20 more
core +4 more sources