Results 41 to 50 of about 2,254,748 (322)
Determination of Protein Concentration Using Bradford Microplate Protein Quantification Assay [PDF]
Disease and Diagnosis, 2015 Background: Bradford protein assay is popular due to its ease of performance and relative sensitivity. Many researchers and laboratories in Iran use standard assay of Bradford by cuvette.
Rouhollah Valipour Nouroozi +2 more
doaj
Site-Differentiated Iron–Sulfur Cluster Ligation Affects Flavin-Based Electron Bifurcation Activity
Metabolites, 2022 Electron bifurcation is an elegant mechanism of biological energy conversion that effectively couples three different physiologically relevant substrates.
Courtney E. Wise +2 more
doaj +1 more source
From omics to AI—mapping the pathogenic pathways in type 2 diabetes
FEBS Letters, EarlyView.Integrating multi‐omics data with AI‐based modelling (unsupervised and supervised machine learning) identify optimal patient clusters, informing AI‐driven accurate risk stratification. Digital twins simulate individual trajectories in real time, guiding precision medicine by matching patients to targeted therapies.
Siobhán O'Sullivan +2 more
wiley +1 more source
Carotenoids: biochemistry, pharmacology and treatment
British Journal of Pharmacology, 2016 Carotenoids and retinoids have several similar biological activities such as antioxidant properties, the inhibition of malignant tumour growth and the induction of apoptosis.
A. Milani +3 more
semanticscholar +1 more source
ERBIN limits epithelial cell plasticity via suppression of TGF‐β signaling
FEBS Letters, EarlyView.In breast and lung cancer patients, low ERBIN expression correlates with poor clinical outcomes. Here, we show that ERBIN inhibits TGF‐β‐induced epithelial‐to‐mesenchymal transition in NMuMG breast and A549 lung adenocarcinoma cell lines. ERBIN suppresses TGF‐β/SMAD signaling and reduces TGF‐β‐induced ERK phosphorylation.
Chao Li +3 more
wiley +1 more source
Rapid subcellular calcium responses and dynamics by calcium sensor G-CatchER+
iScience, 2021 Summary: The precise spatiotemporal characteristics of subcellular calcium (Ca2+) transients are critical for the physiological processes. Here we report a green Ca2+ sensor called “G-CatchER+” using a protein design to report rapid local ER Ca2 ...
Florence N. Reddish +17 more
doaj +1 more source
FEBS Letters, EarlyView.Knowing how proteases recognise preferred substrates facilitates matching proteases to applications. The S1′ pocket of protease EA1 directs cleavage to the N‐terminal side of hydrophobic residues, particularly leucine. The S1′ pocket of thermolysin differs from EA's at only one position (leucine in place of phenylalanine), which decreases cleavage ...
Grant R. Broomfield +3 more
wiley +1 more source
Subchronic Toxicity Evaluation of Ethanol Extract of Picria fel-terrae Lour. Leaf in Wistar Rats
Scientia Pharmaceutica, 2018 Picria fel-terrae extract has been investigated for its hepatoprotective activity. This current study was conducted to evaluate the subchronic toxic effect of Picria fel-terrae Lour. leaf extract. The subchronic toxicity of ethanol extract of P.
Urip Harahap +5 more
doaj +1 more source
Loop-closure principles in protein folding
, 2007 Simple theoretical concepts and models have been helpful to understand the folding rates and routes of single-domain proteins. As reviewed in this article, a physical principle that appears to underly these models is loop closure.Comment: 27 pages, 5 ...
Weikl, Thomas R.
core +1 more source
Exploring lipid diversity and minimalism to define membrane requirements for synthetic cells
FEBS Letters, EarlyView.Designing the lipid membrane of synthetic cells is a complex task, in which its various roles (among them solute transport, membrane protein support, and self‐replication) should all be integrated. In this review, we report the latest top‐down and bottom‐up advances and discuss compatibility and complexity issues of current engineering approaches ...
Sergiy Gan +2 more
wiley +1 more source