Results 61 to 70 of about 1,139,042 (268)

Assessing the stability of uncentrifuged serum and plasma analytes at various post-collection intervals

Journal of Laboratory Medicine
Our study aimed to assess the stability of 26 biochemistry analytes in serum or plasma samples separated from blood samples centrifuged at different time intervals after collection, simulating sample transport via despatch delivery systems.
Mokhsin Atiqah, Subramaniam Poonaresi, Sivaneson Sivasooriar, Nheu Nelson, Ramaloo Gobhy, Hanifah Azana S., Mahathevan Sumitha B., Nadarajah Mohanaraja, Sampasivam Gayathiri, Mohd Ismail Aletza, Abdul Rahman Thuhairah   +10 more
doaj   +1 more source

Les acides gras des organes oppositifoliés de Vitis vinifera L. var. ugni blanc

OENO One, 1975
On sait que les organes oppositifoliés de la Vigne, vrilles et inflorescences, sont assimilés à des rameaux modifiés. Diverses considérations, notamment l'étude des formes de transition entre ces organes, apportent des arguments en faveur de cette ...
Monique Cherrad, Durmus Atalay, Jacques Bouard   +2 more
doaj   +1 more source

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source

High rates of fuel consumption are not required by insulating motifs to suppress retroactivity in biochemical circuits

Engineering Biology, 2017
Retroactivity arises when the coupling of a molecular network [inline-formula] to a downstream network [inline-formula] results in signal propagation back from [inline-formula] to [inline-formula].
Abhishek Deshpande, Thomas E. Ouldridge
doaj   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Elevated Fecal pH Indicates a Profound Change in the Breastfed Infant Gut Microbiome Due to Reduction of Bifidobacterium over the Past Century

mSphere, 2018
Historically, Bifidobacterium species were reported as abundant in the breastfed infant gut. However, recent studies in resource-rich countries show an increased abundance of taxa regarded as signatures of dysbiosis.
Bethany M. Henrick, Andra A. Hutton, Michelle C. Palumbo, Giorgio Casaburi, Ryan D. Mitchell, Mark A. Underwood, Jennifer T. Smilowitz, Steven A. Frese   +7 more
doaj   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Rapamycin and Chloroquine Modulate Insulin Resistance and Hepatic Steatosis in a High-Fat/High-Cholesterol Diet-Induced Metabolic Dysfunction-Associated Steatotic Liver Disease Mouse Model

Diabetes, Metabolic Syndrome and Obesity
Qi Liu,1,* Qian Du,2,* Xiaolu Yuan,3 Ting Zhang,3 Jiajing Li,3 Jingyu Xu,4 Rui Xie2 1Department of Gastroenterology, Zhejiang Provincial People’s Hospital Bijie Hospital, Bijie, Guizhou, People’s Republic of China; 2Department of ...
Liu Q, Du Q, Yuan X, Zhang T, Li J, Xu J, Xie R   +6 more
doaj